LOCUS       V008887                 5811 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008887
VERSION     V008887
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Holkers M, de Vries AA, Goncalves MA.
  TITLE     Nonspaced inverted DNA repeats are preferential targets for
            homology-directed gene repair in mammalian cells
  JOURNAL   Nucleic Acids Res. 40 (5), 1984-1999 (2012)
   PUBMED   22080552
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Holkers M, de Vries AF, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2011) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid-Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 5811)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5811)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "5"; pages:
            "1984-1999"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAR-2011) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid-Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..658
                     /label="chicken beta-actin promoter"
     intron          659..1667
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1762..1782
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1804..1824
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1843..1869
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1873..2577
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     polyA_signal    2742..2797
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3159..3175)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3183..3199)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3207..3237)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3252..3273)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3561..4149)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4323..5180)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5181..5285)
                     /label="AmpR promoter"
     primer_bind     5759..5775
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5809..5811
                     /label="CAG"
                     /note="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
      721 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      781 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      841 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
      961 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1441 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1501 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg
     1561 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1621 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1681 tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaaatt cagatccgcc
     1741 gccactatgg gatcaagatc gccaaaaaag aagagaaagg tgccgaagaa gcatgcagca
     1801 ccaccaaaaa aaaaacgaaa agtagaagac ccacgattta tgtacccata cgatgttcct
     1861 gactatgcgg gtatgaaaaa catcaaaaaa aaccaggtaa tgaacctggg tccgaactct
     1921 aaactgctga aagaatacaa atcccagctg atcgaactga acatcgaaca gttcgaagca
     1981 ggtatcggtc tgatcctggg tgatgcttac atccgttctc gtgatgaagg taaaacctac
     2041 tgtatgcagt tcgagtggaa aaacaaagca tacatggacc acgtatgtct gctgtacgat
     2101 cagtgggtac tgtccccgcc gcacaaaaaa gaacgtgtta accacctggg taacctggta
     2161 atcacctggg gcgcccagac tttcaaacac caagctttca acaaactggc taacctgttc
     2221 atcgttaaca acaaaaaaac catcccgaac aacctggttg aaaactacct gaccccgatg
     2281 tctctggcat actggttcat ggatgatggt ggtaaatggg attacaacaa aaactctacc
     2341 aacaaatcga tcgtactgaa cacccagtct ttcactttcg aagaagtaga atacctggtt
     2401 aagggtctgc gtaacaaatt ccaactgaac tgttacgtaa aaatcaacaa aaacaaaccg
     2461 atcatctaca tcgattctat gtcttacctg atcttctaca acctgatcaa accgtacctg
     2521 atcccgcaga tgatgtacaa actgccgaac actatctcct ccgaaacttt cctgaaataa
     2581 taagtcgatc gaggaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     2641 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     2701 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     2761 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2821 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
     2881 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
     2941 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     3001 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     3061 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
     3121 ccctcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     3181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3301 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3361 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3961 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4621 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4681 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4741 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4801 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4861 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4921 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4981 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5041 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5101 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5161 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5281 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     5341 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     5401 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     5461 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     5521 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     5581 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     5641 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5701 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     5761 aaaacgacgg ccagtgaatt cgagctcggt acccggggat cctctagagt c
//
