LOCUS       V013826                11129 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013826
VERSION     V013826
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          663..1680
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1740..8066
                     /gene="L"
                     /label="RNA-directed RNA polymerase L"
                     /note="RNA-directed RNA polymerase L from Vesicular
                     stomatitis Indiana virus (strain Mudd-Summers). Accession#:
                     Q98776"
     polyA_signal    8217..8272
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        complement(8681..8711)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8725..8746)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8804..9000
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    9006..9140
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9378..9966)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10140..10997)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10998..11102)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgccacca
     1741 tggaagtcca cgattttgag accgacgagt tcaatgattt caatgaagat gactatgcca
     1801 caagagaatt cctgaatccc gatgagcgca tgacgtactt gaatcatgct gattacaatt
     1861 tgaattctcc tctaattagt gatgatattg acaatttgat caggaaattc aattctcttc
     1921 cgattccctc gatgtgggat agtaagaact gggatggagt tcttgagatg ttaacatcat
     1981 gtcaagccaa tcccatctca acatctcaga tgcataaatg gatgggaagt tggttaatgt
     2041 ctgataatca tgatgccagt caagggtata gttttttaca tgaagtggac aaagaggcag
     2101 aaataacatt tgacgtggtg gagaccttca tccgcggctg gggcaacaaa ccaattgaat
     2161 acatcaaaaa ggaaagatgg actgactcat tcaaaattct cgcttatttg tgtcaaaagt
     2221 ttttggactt acacaagttg acattaatct taaatgctgt ctctgaggtg gaattgctca
     2281 acttggcgag gactttcaaa ggcaaagtca gaagaagttc tcatggaacg aacatatgca
     2341 ggattagggt tcccagcttg ggtcctactt ttatttcaga aggatgggct tacttcaaga
     2401 aacttgatat tctaatggac cgaaactttc tgttaatggt caaagatgtg attataggga
     2461 ggatgcaaac ggtgctatcc atggtatgta gaatagacaa cctgttctca gagcaagaca
     2521 tcttctccct tctaaatatc tacagaattg gagataaaat tgtggagagg cagggaaatt
     2581 tttcttatga cttgattaaa atggtggaac cgatatgcaa cttgaagctg atgaaattag
     2641 caagagaatc aaggccttta gtcccacaat tccctcattt tgaaaatcat atcaagactt
     2701 ctgttgatga aggggcaaaa attgaccgag gtataagatt cctccatgat cagataatga
     2761 gtgtgaaaac agtggatctc acactggtga tttatggatc gttcagacat tggggtcatc
     2821 cttttataga ttattacact ggactagaaa aattacattc ccaagtaacc atgaagaaag
     2881 atattgatgt gtcatatgca aaagcacttg caagtgattt agctcggatt gttctatttc
     2941 aacagttcaa tgatcataaa aagtggttcg tgaatggaga cttgctccct catgatcatc
     3001 cctttaaaag tcatgttaaa gaaaatacat ggcccacagc tgctcaagtt caagattttg
     3061 gagataaatg gcatgaactt ccgctgatta aatgttttga aatacccgac ttactagacc
     3121 catcgataat atactctgac aaaagtcatt caatgaatag gtcagaggtg ttgaaacatg
     3181 tccgaatgaa tccgaacact cctatcccta gtaaaaaggt gttgcagact atgttggaca
     3241 caaaggctac caattggaaa gaatttctta aagagattga tgagaagggc ttagatgatg
     3301 atgatctaat tattggtctt aaaggaaagg agagggaact gaagttggca ggtagatttt
     3361 tctccctaat gtcttggaaa ttgcgagaat actttgtaat taccgaatat ttgataaaga
     3421 ctcatttcgt ccctatgttt aaaggcctga caatggcgga cgatctaact gcagtcatta
     3481 aaaagatgtt agattcctca tccggccaag gattgaagtc atatgaggca atttgcatag
     3541 ccaatcacat tgattacgaa aaatggaata accaccaaag gaagttatca aacggcccag
     3601 tgttccgagt tatgggccag ttcttaggtt atccatcctt aatcgagaga actcatgaat
     3661 tttttgagaa aagtcttata tactacaatg gaagaccaga cttgatgcgt gttcacaaca
     3721 acacactgat caattcaacc tcccaacgag tttgttggca aggacaagag ggtggactgg
     3781 aaggtctacg gcaaaaagga tggagtatcc tcaatctact ggttattcaa agagaggcta
     3841 aaatcagaaa cactgctgtc aaagtcttgg cacaaggtga taatcaagtt atttgcacac
     3901 agtataaaac gaagaaatcg agaaacgttg tagaattaca gggtgctctc aatcaaatgg
     3961 tttctaataa tgagaaaatt atgactgcaa tcaaaatagg gacagggaag ttaggacttt
     4021 tgataaatga cgatgagact atgcaatctg cagattactt gaattatgga aaaataccga
     4081 ttttccgtgg agtgattaga gggttagaga ccaagagatg gtcacgagtg acttgtgtca
     4141 ccaatgacca aatacccact tgtgctaata taatgagctc agtttccaca aatgctctca
     4201 ccgtagctca ttttgctgag aacccaatca atgccatgat acagtacaat tattttggga
     4261 catttgctag actcttgttg atgatgcatg atcctgctct tcgtcaatca ttgtatgaag
     4321 ttcaagataa gataccgggc ttgcacagtt ctactttcaa atacgccatg ttgtatttgg
     4381 acccttccat tggaggagtg tcgggcatgt ctttgtccag gtttttgatt agagccttcc
     4441 cagatcccgt aacagaaagt ctctcattct ggagattcat ccatgtacat gctcgaagtg
     4501 agcatctgaa ggagatgagt gcagtatttg gaaaccccga gatagccaag tttcgaataa
     4561 ctcacataga caagctagta gaagatccaa cctctctgaa catcgctatg ggaatgagtc
     4621 cagcgaactt gttaaagact gaggttaaaa aatgcttaat cgaatcaaga caaaccatca
     4681 ggaaccaggt gattaaggat gcaaccatat atttgtatca tgaagaggat cggctcagaa
     4741 gtttcttatg gtcaataaat cctctgttcc ctagattttt aagtgaattc aaatcaggca
     4801 cttttttggg agtcgcagac gggctcatca gtctatttca aaattctcgt actattcgga
     4861 actcctttaa gaaaaagtat catagggaat tggatgattt gattgtgagg agtgaggtat
     4921 cctctttgac acatttaggg aaacttcatt tgagaagggg atcatgtaaa atgtggacat
     4981 gttcagctac tcatgctgac acattaagat acaaatcctg gggccgtaca gttattggga
     5041 caactgtacc ccatccatta gaaatgttgg gtccacaaca tcgaaaagag actccttgtg
     5101 caccatgtaa cacatcaggg ttcaattatg tttctgtgca ttgtccagac gggatccatg
     5161 acgtctttag ttcacgggga ccattgcctg cttatctagg gtctaaaaca tctgaatcta
     5221 catctatttt gcagccttgg gaaagggaaa gcaaagtccc actgattaaa agagctacac
     5281 gtcttagaga tgctatctct tggtttgttg aacccgactc taaactagca atgactatac
     5341 tttctaacat ccactcttta acaggcgaag aatggaccaa aaggcagcat gggttcaaaa
     5401 gaacagggtc tgcccttcat aggttttcga catctcggat gagccatggt gggttcgcat
     5461 ctcagagcac tgcagcattg accaggttga tggcaactac agacaccatg agggatctgg
     5521 gagatcagaa tttcgacttt ttattccaag caacgttgct ctatgctcaa attaccacca
     5581 ctgttgcaag agacggatgg atcaccagtt gtacagatca ttatcatatt gcctgtaagt
     5641 cctgtttgag acccatagaa gagatcaccc tggactcaag tatggactac acgcccccag
     5701 atgtatccca tgtgctgaag acatggagga atggggaagg ttcgtgggga caagagataa
     5761 aacagatcta tcctttagaa gggaattgga agaatttagc acctgctgag caatcctatc
     5821 aagtcggcag atgtataggt tttctatatg gagacttggc gtatagaaaa tctactcatg
     5881 ccgaggacag ttctctattt cctctatcta tacaaggtcg tattagaggt cgaggtttct
     5941 taaaagggtt gctagacgga ttaatgagag caagttgctg ccaagtaata caccggagaa
     6001 gtctggctca tttgaagagg ccggccaacg cagtgtacgg aggtttgatt tacttgattg
     6061 ataaattgag tgtatcacct ccattccttt ctcttactag atcaggacct attagagacg
     6121 aattagaaac gattccccac aagatcccaa cctcctatcc gacaagcaac cgtgatatgg
     6181 gggtgattgt cagaaattac ttcaaatacc aatgccgtct aattgaaaag ggaaaataca
     6241 gatcacatta ttcacaatta tggttattct cagatgtctt atccatagac ttcattggac
     6301 cattctctat ttccaccacc ctcttgcaaa tcctatacaa gccattttta tctgggaaag
     6361 ataagaatga gttgagagag ctggcaaatc tttcttcatt gctaagatca ggagaggggt
     6421 gggaagacat acatgtgaaa ttcttcacca aggacatatt attgtgtcca gaggaaatca
     6481 gacatgcttg caagttcggg attgctaagg ataataataa agacatgagc tatccccctt
     6541 ggggaaggga atccagaggg acaattacaa caatccctgt ttattatacg accacccctt
     6601 acccaaagat gctagagatg cctccaagaa tccaaaatcc cctgctgtcc ggaatcaggt
     6661 tgggccaatt accaactggc gctcattata aaattcggag tatattacat ggaatgggaa
     6721 tccattacag ggacttcttg agttgtggag acggctccgg agggatgact gctgcattac
     6781 tacgagaaaa tgtgcatagc agaggaatat tcaatagtct gttagaatta tcagggtcag
     6841 tcatgcgagg cgcctctcct gagcccccca gtgccctaga aactttagga ggagataaat
     6901 cgagatgtgt aaatggtgaa acatgttggg aatatccatc tgacttatgt gacccaagga
     6961 cttgggacta tttcctccga ctcaaagcag gcttggggct tcaaattgat ttaattgtaa
     7021 tggatatgga agttcgggat tcttctacta gcctgaaaat tgagacgaat gttagaaatt
     7081 atgtgcaccg gattttggat gagcaaggag ttttaatcta caagacttat ggaacatata
     7141 tttgtgagag cgaaaagaat gcagtaacaa tccttggtcc catgttcaag acggtcgact
     7201 tagttcaaac agaatttagt agttctcaaa cgtctgaagt atatatggta tgtaaaggtt
     7261 tgaagaaatt aatcgatgaa cccaatcccg attggtcttc catcaatgaa tcctggaaaa
     7321 acctgtacgc attccagtca tcagaacagg aatttgccag agcaaagaag gttagtacat
     7381 actttacctt gacaggtatt ccctcccaat tcattcctga tccttttgta aacattgaga
     7441 ctatgctaca aatattcgga gtacccacgg gtgtgtctca tgcggctgcc ttaaaatcat
     7501 ctgatagacc tgcagattta ttgaccatta gcctttttta tatggcgatt atatcgtatt
     7561 ataacatcaa tcatatcaga gtaggaccga tacctccgaa ccccccatca gatggaattg
     7621 cacaaaatgt ggggatcgct ataactggta taagcttttg gctgagtttg atggagaaag
     7681 acattccact atatcaacag tgtttggcag ttatccagca atcatttccg attaggtggg
     7741 aggctatttc agtaaaagga ggatacaagc agaagtggag tactagaggt gatgggctcc
     7801 caaaagatac ccgaatttca gactccttgg ccccaatcgg gaactggatc agatctttgg
     7861 aattggtccg aaaccaagtt cgtctaaatc cattcaataa gatcttgttc aatcagctat
     7921 gtcgtacagt ggataatcat ttgaagtggt caaatttgcg aaaaaacaca ggaatgattg
     7981 aatggatcaa tgggcgaatt tcaaaagaag accggtctat actgatgttg aagagtgacc
     8041 tacatgagga aaactcttgg agagattaaa attcactcct caggtgcagg ctgcctatca
     8101 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     8161 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     8221 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     8281 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     8341 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     8401 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     8461 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     8521 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     8581 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     8641 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8701 taaagtgtaa agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8761 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     8821 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8881 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8941 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9001 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9121 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9781 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11101 cgcacatttc cccgaaaagt gccacctgg
//
