LOCUS       40924_8036        9890 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAG-Gal4VP16-2A-TagBFP-2A-Bla, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 9890)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(984..1841)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1842..1946)
                     /label=AmpR promoter
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2595..2613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     enhancer        4313..4616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4619..4894
                     /label=chicken beta-actin promoter
     intron          4900..5908
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     protein_bind    5966..5990
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6010..6019
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6019..6252
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             6259..6696
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    6697..6762
                     /label=P2A
                     /note="P2A"
     CDS             6706..6762
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6769..7464
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     misc_feature    7465..7518
                     /label=2A
                     /note="2A"
     CDS             7465..7518
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7519..7914
                     /label=BSD
                     /note="blasticidin S deaminase"
     protein_bind    complement(7923..7947)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8102..8157
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8518..8534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8542..8558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8566..8596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8611..8632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    8805..9641
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(9671..9689)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9710..9726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9734..9750)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9758..9788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9803..9824)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
       61 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1981 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2401 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     2461 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2521 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2581 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgc tttctctgac
     2641 cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc
     2701 tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt cctccactcc
     2761 ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg agcacttcct
     2821 tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct gggtcctctc
     2881 cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg ttcccttttc
     2941 cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg
     3001 tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca
     3061 accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc
     3121 ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct
     3181 cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc
     3241 ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact
     3301 tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat
     3361 attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc ccctccaccc
     3421 cacagtgggg caagcttatt accctgttat ccctaaccgg tttaccgagc tcttattggt
     3481 tttcaaactt cattgactgt gccaagcttc tgacctcttc tcttcctccc acagggcctc
     3541 gagacaattg catcgatggt accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg
     3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4141 tccccgtgag ctcccaggcg cgccaactcg gtaccgtatc gatgtcgacg ttaacgctag
     4201 tgatatcaac tttgtataga aaagttggct ccgaatttct cgagttaatt aatagttatt
     4261 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     4321 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     4381 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     4441 actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     4501 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     4561 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatgggtc
     4621 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     4681 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
     4741 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
     4801 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     4861 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gagtcgctgc gttgccttcg
     4921 ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     4981 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     5041 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
     5101 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     5161 cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     5221 gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     5281 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     5341 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     5401 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     5461 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     5521 cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     5581 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     5641 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg gcgaagcggt
     5701 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     5761 cccttctcca tctccagcct cggggctgcc gcagggggac ggctgccttc gggggggacg
     5821 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     5881 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
     5941 tcatcatttt ggcaaagaat tcgacccaag tttgtacaaa aaagcaggct ggatccccgg
     6001 gtaccggtcg ccaccatggc ccccccgacc gatgtcagcc tgggggacga gctccactta
     6061 gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg
     6121 ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc cccctacggc
     6181 gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac
     6241 gagtacggtg ggacgcgtat gaagctactg tcttctatcg aacaagcatg cgatatttgc
     6301 cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac
     6361 aactgggagt gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg
     6421 acagaagtgg aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga
     6481 gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca
     6541 ggattatttg tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg
     6601 gagactgata tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa
     6661 gagagtagta acaaaggtca aagacagttg actgtagggt ccggagctac aaatttttcc
     6721 ctcctcaaac aggctggaga tgtcgaagaa aatcctgggc ctactagtat gagcgagctg
     6781 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     6841 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     6901 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     6961 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     7021 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     7081 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     7141 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     7201 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     7261 gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct
     7321 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     7381 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     7441 cctagcaaac tggggcacca attggagggc cgcggcagcc tgctgacctg cggcgacgtg
     7501 gaggaaaacc ccggccccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     7561 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     7621 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     7681 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     7741 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     7801 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     7861 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaaata
     7921 ccacccagct ttcttgtaca aagtggtacg cgtgaattca ctcctcaggt gcaggctgcc
     7981 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     8041 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     8101 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     8161 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     8221 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     8281 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     8341 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     8401 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     8461 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     8521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcg acagtactaa
     8701 gcttggtgcg tttttatgct tgtagtattg tataatgttt ttaagatcct taattaatag
     8761 ggataacagg gtaatctcga ggtttaaacg tcgacgcggc cgctactagg gacaggattg
     8821 gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa
     8881 cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca
     8941 tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg
     9001 agggagagct tggcaggggg tgggagggaa gggggggatg cgtgacctgc ccggttctca
     9061 gtggccaccc tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg
     9121 cgtttcactg atcctggtgc tgcagcttcc ttacacttcc caagaggaga agcagtttgg
     9181 aaaaacaaaa tcagaataag ttggtcctga gttctaactt tggctcttca cctttctagt
     9241 ccccaattta tattgttcct ccgtgcgtca gttttacctg tgagataagg ccagtagcca
     9301 gccccgtcct ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt
     9361 gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc ctctactccc tttctctttc
     9421 tccatccttc tttccttaaa gagtccccag tgctatctgg gacatattcc tccgcccaga
     9481 gcagggtccc gcttccctaa ggccctgctc tgggcttctg ggtttgagtc cttggcaagc
     9541 ccaggagagg cgctcaggct tccctgtccc ccttcctcgt ccaccatctc atgcccctgg
     9601 ctctcctgcc ccttccctac aggggttcct ggctctgctc tccaccgcgg tggagctcca
     9661 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     9721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
//
