LOCUS       40924_7956        5699 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCA13-DsRed-IRES-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Li I-C., Chiu C-Y., Wu C-L., Chi J-Y., Jian S-R., Wang S-W., Chang 
            CL.
  TITLE     A dual-fluorescent reporter system facilitates identification of 
            thiol compounds that suppress oxidative frameshift mutations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Chiu C-Y., Chang CL.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2012) Institute of Molecular Medicine, National 
            Cheng Kung University, 1 Ta-Hsueh Road, Tainan, Taiwan 70101, 
            Republic of China
REFERENCE   3  (bases 1 to 5699)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5699)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-2012) Institute of Molecular Medicine, National Cheng Kung 
            University, 1 Ta-Hsueh Road, Tainan, Taiwan 70101, Republic of 
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   660..685
                     /rpt_unit_seq="ca"
                     /satellite="microsatellite"
     CDS             707..1384
                     /label=DsRed1
                     /note="wild-type DsRed"
     CDS             1436..1507
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags"
     misc_feature    1543..2126
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2127..2843
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
     promoter        complement(2877..2895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3169..3290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3297..3752)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3779..3881
                     /label=AmpR promoter
     protein_bind    3898..3931
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3976..4833)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5003..5591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctcatgc
      661 acacacacac acacacacac acacagtacg cgtaccggtc gccaccatgg tgcgctcctc
      721 caagaacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg
      781 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggcc acaacaccgt
      841 gaagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
      901 gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa
      961 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     1021 ggtgaccgtg acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt
     1081 catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     1141 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc
     1201 cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa
     1261 gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca
     1321 caacgaggac tacaccatcg tggagcagta cgagcgcacc gagggccgcc accacctgtt
     1381 cctgtagcgg ccgctctagc ccgggcggat ccgaattcgc atgcgtcgac tcgaggacta
     1441 caaggatgac gatgacaagg attacaaaga cgacgatgat aaggactata aggatgatga
     1501 cgacaaataa tagcaattcc tcgacgactg catagggtta cccccctctc cctccccccc
     1561 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     1621 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     1681 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     1741 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     1801 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     1861 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     1921 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     1981 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     2041 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     2101 aaaacacgat gataatggcc acaaccatgg tgagcaagca gatcctgaag aacaccggcc
     2161 tgcaggagat catgagcttc aaggtgaacc tggagggcgt ggtgaacaac cacgtgttca
     2221 ccatggaggg ctgcggcaag ggcaacatcc tgttcggcaa ccagctggtg cagatccgcg
     2281 tgaccaaggg cgcccccctg cccttcgcct tcgacatcct gagccccgcc ttccagtacg
     2341 gcaaccgcac cttcaccaag taccccgagg acatcagcga cttcttcatc cagagcttcc
     2401 ccgccggctt cgtgtacgag cgcaccctgc gctacgagga cggcggcctg gtggagatcc
     2461 gcagcgacat caacctgatc gaggagatgt tcgtgtaccg cgtggagtac aagggccgca
     2521 acttccccaa cgacggcccc gtgatgaaga agaccatcac cggcctgcag cccagcttcg
     2581 aggtggtgta catgaacgac ggcgtgctgg tgggccaggt gatcctggtg taccgcctga
     2641 acagcggcaa gttctacagc tgccacatgc gcaccctgat gaagagcaag ggcgtggtga
     2701 aggacttccc cgagtaccac ttcatccagc accgcctgga gaagacctac gtggaggacg
     2761 gcggcttcgt ggagcagcac gagaccgcca tcgcccagct gaccagcctg ggcaagcccc
     2821 tgggcagcct gcacgagtgg gtgtaatagg gtaccaggta agtgtaccca attcgcccta
     2881 tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg
     2941 cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     3001 gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc
     3061 atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3121 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3181 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3241 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     3301 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3361 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3421 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3481 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3541 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3601 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3661 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3721 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3841 ataaccctga taaatgcttc aataatattg aaaaaggaag aatcctgagg ccgggccata
     3901 acttcgtata atgtatgcta tacgaagtta tccatgggcc ccccctcgac atgagtaaac
     3961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4261 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4561 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4801 aataagggcg acacggaaat gttgaatact catactcttc ctcaggttac tcatatatac
     4861 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4921 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5641 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcc
//
