LOCUS       40924_7941       12759 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Chloroplast transformation vector pC35-STDBxb1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Shao M, Kumar S, Thomson JG.
  TITLE     Precise excision of plastid DNA by the large serine recombinase Bxb1
  JOURNAL   Plant Biotechnol. J. (2013) In press
  PUBMED    24261912
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   Shao M, Kumar S, Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2013) Crop Improvement and Utilization, USDA, 800 
            Buchanan Street, Albany, CA 94710, USA
REFERENCE   3  (bases 1 to 12759)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12759)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J. (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2013) Crop Improvement and Utilization, USDA, 800 Buchanan 
            Street, Albany, CA 94710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(82..106)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     309..325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1571..1914
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1938..3620
                     /codon_start=1
                     /gene="STDBxb1"
                     /product="STDBxb1"
                     /label=STDBxb1
                     /note="Bxb1 large serine recombinase/integrase from 
                     Mycobacterium smegmati bacteriophage Bxb1; similar to INSD 
                     Accession Number JQ305141.1; stroma targeting domain (STD) 
                     fused to Bxb1 large serine recombinase"
                     /protein_id="AHB17669.1"
                     /translation="MASSLLSSAAVATRSNVAQANMVAPFTGLKSAASFPVSRKQNLDI
                     TSIASNGGRVQCMLINRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDL
                     DVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKL
                     VVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWG
                     YLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQ
                     GREPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRA
                     ELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGT
                     VAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRA
                     GSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRS
                     MNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     gene            1938..3620
                     /gene="STDBxb1"
                     /label=STDBxb1
     sig_peptide     1938..2111
                     /gene="STDBxb1"
                     /note="tobacco gene for ribulose 1,5-bisphosphate
                     carboxylase small subunit stroma targeting domain (STD); 
                     similar to INSD Accession Number X02353.1"
     terminator      3795..4047
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4411..4427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4435..4451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4459..4489)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4504..4525)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4716..5393
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             5462..6250
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6310..6484
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(6562..6586)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7011..7802
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7892..8480
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8666..8806)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9150..9344)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9413..10477)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10914..11540)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta
       61 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
      121 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
      181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      241 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      301 acgacgttgt aaaacgacgg ccagtgccaa gcttagcaag agctaacggc tcttcgcaac
      361 aaggagacct ccctctccac cgtgaaacac cggtaaaaga agaagcagtt cattctgcac
      421 tggccacttt tacgccaact caagtaaagg ctattccaga gcaaacggct cctggtaaag
      481 aatcaacaaa tccgttgatg gctagtatct tgccaaaaga tatgaaccca gttcaaactg
      541 ggataaggct tgcagtgcca ggggactttt tacgtcctca tcagggaatt ccaatcccac
      601 aaaaatctga gcttagcagc atagttgctc ctctcagagc agaatcgggt attcaccacc
      661 ctcatatcaa ctactacgtt gtgtataacg gtccacacgc cggtatatac gatgactggg
      721 gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc atacaagaag tttgccacta
      781 ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca gacaggttga
      841 acttcatccc caaaggagaa gctcaactca agcccaagag ctttgcgaag gccttaacca
      901 gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc agcagtgatc
      961 cagccccaaa agagatctcc tttgccccgg agatcaccat ggacgacttt ctctatctct
     1021 acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc accgataatg
     1081 agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
     1141 acgcggcagg tctcatcaag acgatctacc cgagtaataa tctccaggag atcaaatacc
     1201 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
     1261 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     1321 ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt cctactgaat
     1381 caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc gtgaagactg
     1441 gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
     1501 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     1561 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     1621 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     1681 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     1741 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     1801 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     1861 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggatctgct
     1921 ctagggcgcg ccccaccatg gcttcctcac ttctttcctc tgcagcagtt gccacccgca
     1981 gcaatgttgc tcaagctaac atggttgcac ctttcactgg ccttaagtca gctgcctcat
     2041 tccctgtttc aaggaagcaa aaccttgaca tcacttccat tgccagcaac ggcggaagag
     2101 tgcaatgcat gttaattaac agagccctgg tagtcatccg cctgtcccgc gtcaccgatg
     2161 ctacgacttc accggagcgt cagctggagt cttgccagca gctctgcgcc cagcgcggct
     2221 gggacgtcgt cggggtagcg gaggatctgg acgtctccgg ggcggtcgat ccgttcgacc
     2281 ggaagcgcag accgaacctg gcccggtggc tagcgttcga ggagcaaccg ttcgacgtga
     2341 tcgtggcgta ccgggtagac cggttgaccc gatcgatccg gcatctgcag cagctggtcc
     2401 actgggccga ggaccacaag aagctggtcg tctccgcgac cgaagcgcac ttcgatacga
     2461 cgacgccgtt tgcggcggtc gtcatcgcgc ttatgggaac ggtggcgcag atggaattag
     2521 aagcgatcaa agagcggaac cgttcggctg cgcatttcaa tatccgcgcc gggaaatacc
     2581 gaggatccct gccgccgtgg ggatacctgc ctacgcgcgt ggacggggag tggcggctgg
     2641 tgccggaccc tgtgcagcga gagcgcatcc tcgaggtgta tcaccgcgtc gtcgacaacc
     2701 acgagccgct gcacctggtg gcccacgacc tgaaccggcg tggtgtcctg tcgccgaagg
     2761 actacttcgc gcagctgcaa ggccgcgagc cgcagggccg ggagtggtcg gctaccgcgc
     2821 tgaagcgatc gatgatctcc gaggcgatgc tcgggtacgc gactctgaac ggtaagaccg
     2881 tccgagacga cgacggagcc ccgctggtgc gggctgagcc gatcctgacc cgtgagcagc
     2941 tggaggcgct gcgcgccgag ctcgtgaaga cctcccgggc gaagcccgcg gtgtctaccc
     3001 cgtcgctgct gctgcgggtg ttgttctgcg cggtgtgcgg ggagcccgcg tacaagttcg
     3061 ccgggggagg acgtaagcac ccgcgctacc gctgccgctc gatggggttc ccgaagcact
     3121 gcgggaacgg cacggtggcg atggccgagt gggacgcgtt ctgcgaggag caggtgctgg
     3181 atctgctcgg ggacgcggag cgtctggaga aagtctgggt agccggctcg gactccgcgg
     3241 tcgaactcgc ggaggtgaac gcggagctgg tggacctgac gtcgctgatc ggctccccgg
     3301 cctaccgggc cggctctccg cagcgagaag cactggatgc ccgtattgcg gcgctggccg
     3361 cgcggcaaga ggagctggag ggtctagagg ctcgcccgtc tggctgggag tggcgcgaga
     3421 ccgggcagcg gttcggggac tggtggcggg agcaggacac cgcggcaaag aacacctggc
     3481 ttcggtcgat gaacgttcgg ctgacgttcg acgtccgcgg cgggctgact cgcacgatcg
     3541 acttcgggga tctgcaggag tacgagcagc atctcaggct cggcagcgtg gtcgaacggc
     3601 tacacaccgg gatgtcgtag actagtgcat gcattcatca atattattca tgcggggaaa
     3661 ggcaagatta atccaactgg caaatcatcc agcgtgattg gtaacttcag ttccagcgac
     3721 ttgattcgtt ttggtgctac ccacgttttc aataaggacg agatggtgga gtaaagaagg
     3781 agtgcgtcga agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     3841 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     3901 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     3961 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     4021 gcgcggtgtc atctatgtta ctagatcgat caaacttcgg cactgtgtaa tgacgatgag
     4081 caatcgagag gctgactaac aaaaggtatg cccaaaaaca acctctccaa actgtttcga
     4141 attggaagtt tctgctcatg ccgacaggca taacttagat attcgcgggc tattcccact
     4201 aattcgtcct gctggtttgc gccaagataa atcagtgcat ctccttacaa gttcctctgt
     4261 cttgtgaaat gaactgctga ctgcccccca agaaagcctc ctcatctccc agttggcggc
     4321 ggctgataca ccatcgaaaa cccacgtccg aacacttgat acatgtgcct gagaaatagg
     4381 ggtcgacccg agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4441 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4501 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4561 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4621 tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     4681 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     4741 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     4801 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     4861 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     4921 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
     4981 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     5041 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     5101 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
     5161 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
     5221 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     5281 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     5341 caagaccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc
     5401 agtctctctc tacaaatcta tctctctcga gctttcgcag atctgtcgat cgaccatggg
     5461 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5521 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5581 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
     5641 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5701 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5761 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5821 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5881 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5941 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6001 cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6061 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6121 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6181 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6241 cgagttcttc tgagcgggac tctggggttc ggatcgatcc tctagctaga gtcgatcgac
     6301 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     6361 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     6421 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     6481 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     6541 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     6601 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     6661 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     6721 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     6781 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     6841 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     6901 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     6961 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     7021 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     7081 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     7141 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     7201 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     7261 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     7321 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     7381 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     7441 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     7501 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     7561 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     7621 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     7681 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     7741 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     7801 tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8641 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8701 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8761 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     8821 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8881 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8941 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     9001 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     9061 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     9121 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     9181 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     9241 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     9301 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     9361 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     9421 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     9481 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     9541 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     9601 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     9661 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     9721 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     9781 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     9841 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     9901 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     9961 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
    10021 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
    10081 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
    10141 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
    10201 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
    10261 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
    10321 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
    10381 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
    10441 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg
    10501 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    10561 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    10621 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    10681 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    10741 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    10801 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    10861 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    10921 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    10981 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    11041 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    11101 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    11161 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    11221 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    11281 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    11341 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    11401 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    11461 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    11521 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    11581 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    11641 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    11701 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    11761 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    11821 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    11881 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    11941 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    12001 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    12061 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    12121 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    12181 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    12241 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    12301 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    12361 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    12421 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    12481 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    12541 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    12601 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    12661 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    12721 agtactttga tcccgagggg aaccctgtgg ttggcatgg
//
