LOCUS       40924_7936       11900 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Transformation vector pC23LG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Ma BG, Duan XY, Niu JX, Ma C, Zhang LX, Hao QN, Zhang HP.
  TITLE     Construction of selectable marker-free plant transformation vectors 
            using inducible Cre/loxP site-specific recombination system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Ma BG, Duan XY, Niu JX, Ma C, Zhang LX, Hao QN, Zhang HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2007) Department of Horticulture, Agriculture 
            College, Shihezi University, Shihezi, Xin Jiang 832003, China
REFERENCE   3  (bases 1 to 11900)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11900)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2007) Department of Horticulture, Agriculture College, 
            Shihezi University, Shihezi, Xin Jiang 832003, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2282..3346
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3415..3609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6173..6197
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6509..7297)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7366..8043)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8234..8255
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8270..8300
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8308..8324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8332..8348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(8408..8441)
                     /label=loxP site
                     /note="loxP site"
     protein_bind    8408..8441
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     terminator      complement(8467..8719)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8793..10601)
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(10640..10985)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    complement(11505..11538)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11576..11592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11795..11819
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
     6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg
     6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg
     7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct
     7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
     7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
     7801 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     7861 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     7921 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     7981 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     8041 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     8101 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8341 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctagt ggatctgata
     8401 tcacctaata acttcgtata gcatacatta tacgaagtta tattaagggt tctcgagaga
     8461 attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
     8521 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
     8581 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
     8641 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
     8701 gccaaatgtt tgaacgatcg gggaaattcg agctcggtag caattcccga ggctgtagcc
     8761 gacgatggtg cgccaggaga gttgttgatt cattgtttgc ctccctgctg cggtttttca
     8821 ccgaagttca tgccagtcca gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg
     8881 tcgcgagtga agatcccttt cttgttaccg ccaacgcgca atatgccttg cgaggtcgca
     8941 aaatcggcga aattccatac ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg
     9001 tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt gtacattgag
     9061 tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga taatcggctg atgcagtttc
     9121 tcctgccagg ccagaagttc tttttccagt accttctctg ccgtttccaa atcgccgctt
     9181 tggacatacc atccgtaata acggttcagg cacagcacat caaagagatc gctgatggta
     9241 tcggtgtgag cgtcgcagaa cattacattg acgcaggtga tcggacgcgt cgggtcgagt
     9301 ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc
     9361 ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg cgctatcagc
     9421 tctttaatcg cctgtaagtg cgcttgctga gtttccccgt tgactgcctc ttcgctgtac
     9481 agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca
     9541 gcagcagttt catcaatcac cacgatgcca tgttcatctg cccagtcgag catctcttca
     9601 gcgtaagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat taatgcgtgg
     9661 tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca agtccgcatc ttcatgacga
     9721 ccaaagccag taaagtagaa cggtttgtgg ttaatcagga actgttcgcc cttcactgcc
     9781 actgaccgga tgccgacgcg aagcgggtag atatcacact ctgtctggct tttggctgtg
     9841 acgcacagtt catagagata accttcaccc ggttgccaga ggtgcggatt caccacttgc
     9901 aaagtcccgc tagtgccttg tccagttgca accacctgtt gatccgcatc acgcagttca
     9961 acgctgacat caccattggc caccacctgc cagtcaacag acgcgtggtt acagtcttgc
    10021 gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt
    10081 acgctgcgat ggattccggc atagttaaag aaatcatgga agtaagactg ctttttcttg
    10141 ccgttttcgt cggtaatcac cattcccggc gggatagtct gccagttcag ttcgttgttc
    10201 acacaaacgg tgatacgtac acttttcccg gcaataacat acggcgtgac atcggcttca
    10261 aatggcgtat agccgccctg atgctccatc acttcctgat tattgaccca cactttgccg
    10321 taatgagtga ccgcatcgaa acgcagcacg atacgctggc ctgcccaacc tttcggtata
    10381 aagacttcgc gctgatacca gacgttgccc gcataattac gaatatctgc atcggcgaac
    10441 tgatcgttaa aactgcctgg cacagcaatt gcccggcttt cttgtaacgc gctttcccac
    10501 caacgctgat caattccaca gttttcgcga tccagactga atgcccacag gccgtcgagt
    10561 tttttgattt cacgggttgg ggtttctaca ggacgtaaca taagggactg accacccggg
    10621 gatcctctag agtcccccgt gttctctcca aatgaaatga acttccttat atagaggaag
    10681 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    10741 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    10801 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttcaacgatg gcctttcctt
    10861 tatcgcaatg atggcatttg taggagccac cttccttttc cactatcttc acaataaagt
    10921 gacagatagc tgggcaatgg aatccgagga ggtttccgga tattaccctt tgttgaaaag
    10981 tctcaattgc cctttggtct tctgagactg tatctttgat atttttggag tagacaagtg
    11041 tgtcgtgctc caccatgttg acgaagattt tcttcttgtc attgagtcgt aagagactct
    11101 gtatgaactg ttcgccagtc tttacggcga gttctgttag gtcctctatt tgaatctttg
    11161 actccatggc ctttgattca gtgggaacta cctttttaga gactccaatc tctattactt
    11221 gccttggttt gtgaagcaag ccttgaatcg tccatactgg aatagtactt ctgatcttga
    11281 gaaatatatc tttctctgtg ttcttgatgc agttagtcct gaatcttttg actgcatctt
    11341 taaccttctt gggaaggtat ttgatttcct ggagattatt gctcgggtag atcgtcttga
    11401 tgagacctgc tgcgtaagcc tctctaacca tctgtgggtt agcattcttt ctgaaattga
    11461 aaaggctaat ctggggacct gcaggcatgc aagctctaga cctaataact tcgtatagca
    11521 tacattatac gaagttatat taagggttgt cgacctgcag gcatgcaagc ttggcactgg
    11581 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    11641 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    11701 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
    11761 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
    11821 taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc
    11881 cgttcgtcca tttgtatgtg
//
