LOCUS       .                       9470 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(160..184)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     387..403
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             755..1777
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     polyA_signal    1787..1961
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2048..2064)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2072..2088)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2096..2126)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2141..2162)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2353..3028
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(3352..3376)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3801..4592
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4682..5270
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5456..5596)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5940..6134)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6203..7267)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7704..8330)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
       61 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
      121 taaatatccg ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
      181 gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct
      241 gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      301 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      361 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct
      421 gcaggtcgac tctagaggag gtcaacacat caatgcctat tttggtttag tcgtccaggc
      481 ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat ttaggcaact ggtcagatca
      541 gccccacttg tagcagtagc ggcggcgctc gaagtgtgac tcttattagc agacaggaac
      601 gaggacatta ttatcatctg ctgcttggtg cacgataact tggtgcgttt gtcaagcaag
      661 gtaagtggac gacccggtca taccttctta agttcgccct tcctcccttt atttcagatt
      721 caatctgact tacctattct acccaagcat ccaaatgaaa aagcctgaac tcaccgcgac
      781 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
      841 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
      901 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
      961 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
     1021 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     1081 cgctgttctc cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     1141 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     1201 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     1261 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     1321 cgaagtccgg cacctcgtgc atgcggattt cggctccaac aatgtcctga cggacaatgg
     1381 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     1441 cgccaacatc ctcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     1501 cgagcggagg catccggagc ttgcaggatc gccgcgcctc cgggcgtata tgctccgcat
     1561 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     1621 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     1681 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     1741 aaaccgacgc cccagcactc gtccgagggc aaaggaatag ctcgagtttc tccataataa
     1801 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
     1861 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     1921 tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt
     1981 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     2041 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2101 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2161 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2221 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa
     2281 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     2341 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     2401 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     2461 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
     2521 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     2581 ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc tactccaaga
     2641 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     2701 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     2761 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     2821 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     2881 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     2941 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga ccttcctcta tataaggaag
     3001 ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct
     3061 ctcgaggatc cactagtccg ggtaccgagc tcgaattcgt ttctccataa taatgtgtga
     3121 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     3181 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     3241 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
     3301 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     3361 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     3421 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     3481 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     3541 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     3601 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     3661 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     3721 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
     3781 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
     3841 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
     3901 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
     3961 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     4021 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     4081 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     4141 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     4201 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     4261 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     4321 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     4381 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     4441 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     4501 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     4561 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
     4621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4801 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5341 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5401 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     5461 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     5521 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     5581 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5641 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5701 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     5761 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     5821 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     5881 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     5941 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     6001 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     6061 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     6121 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     6181 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     6241 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     6301 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     6361 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     6421 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     6481 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     6541 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     6601 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     6661 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     6721 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     6781 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     6841 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     6901 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     6961 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     7021 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     7081 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     7141 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     7201 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     7261 tgcgaccgtc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     7321 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     7381 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     7441 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     7501 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     7561 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     7621 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     7681 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     7741 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     7801 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     7861 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     7921 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     7981 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     8041 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     8101 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     8161 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     8221 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     8281 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     8341 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     8401 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     8461 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     8521 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     8581 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     8641 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     8701 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     8761 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     8821 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     8881 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     8941 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     9001 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     9061 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     9121 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     9181 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     9241 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     9301 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     9361 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     9421 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
//
