LOCUS       40924_7911        9182 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC1300intC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Ouwerkerk PBF.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute 
            for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   2  (bases 1 to 9182)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2000) Dept. of Molecular Cell Biology, Institute for 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..69
                     /note="multiple cloning site; identical to pCAMBIA-1300
                     except that additional unique restriction sites BglII and 
                     XhoI are present"
     primer_bind     complement(73..89)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    292..316
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1616..2242
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2679..3743
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3812..4006
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4350..4490
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4676..5264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5354..6145)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6570..6594
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6672..6846)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(6897..7589)
                     /gene="hptII"
                     /number=2
     intron          complement(7584..7773)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(7780..8112)
                     /gene="hptII"
                     /number=1
     promoter        complement(8191..8868)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9059..9080
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9095..9125
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9133..9149
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9157..9173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcagat ctctcgagga gctcggtacc cggggatcct ctagagtcga cctgcaggca
       61 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      121 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      181 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
      241 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
      301 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg
      361 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
      421 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
      481 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
      541 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
      601 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
      661 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
      721 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
      781 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
      841 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
      901 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
      961 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     1021 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     1081 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     1141 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     1201 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     1261 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     1321 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     1381 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     1441 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     1501 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     1561 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     1621 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     1681 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     1741 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     1801 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     1861 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     1921 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     1981 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     2041 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     2101 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     2161 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     2221 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     2281 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     2341 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     2401 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     2461 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     2521 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     2581 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
     2641 tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg
     2701 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     2761 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     2821 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     2881 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     2941 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     3001 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     3061 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     3121 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     3181 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     3241 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     3301 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     3361 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     3421 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     3481 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     3541 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     3601 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     3661 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     3721 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     3781 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
     3841 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     3901 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     3961 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     4021 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     4081 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     4141 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     4201 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4261 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4321 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     4381 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     4441 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     4501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5101 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     5341 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     5401 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     5461 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     5521 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     5581 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     5641 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     5701 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     5761 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     5821 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     5881 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     5941 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     6001 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     6061 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     6121 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     6181 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     6241 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     6301 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     6361 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     6421 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     6481 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     6541 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     6601 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     6661 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     6721 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     6781 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     6841 gagaaactcg aagatccgtc gagcttgtcg atcgacagat ccggtcggca tctactctat
     6901 ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     6961 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     7021 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     7081 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt
     7141 cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     7201 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     7261 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     7321 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     7381 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     7441 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     7501 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     7561 gcatccatgg cctccgcgac cggctgcagt tatcatcatc atcatagaca cacgaaataa
     7621 agtaatcaga ttatcagtta aagctatgta atatttacac cataaccaat caattaaaaa
     7681 atagatcagt ttaaagaaag atcaaagctc aaaaaaataa aaagagaaaa gggtcctaac
     7741 caagaaaatg aaggagaaaa actagaaatt tacctgcaga acagcgggca gttcggtttc
     7801 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
     7861 gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
     7921 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
     7981 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
     8041 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
     8101 aggctttttc atatctcatt gcccccccgg atctgcgaaa gctcgacgga tcttcgagag
     8161 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
     8221 tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     8281 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     8341 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     8401 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     8461 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     8521 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     8581 ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa
     8641 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
     8701 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
     8761 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
     8821 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
     8881 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
     8941 ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9001 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9061 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9121 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9181 ac
//
