LOCUS       40924_7906        9170 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC1300intB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Ouwerkerk PBF.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute 
            for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   2  (bases 1 to 9170)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9170)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2000) Dept. of Molecular Cell Biology, Institute for 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    280..304
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1604..2230
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2667..3731
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3800..3994
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4338..4478
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4664..5252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5342..6133)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6558..6582
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6660..6834)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(6885..7577)
                     /gene="hptII"
                     /number=2
     intron          complement(7572..7761)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(7768..8100)
                     /gene="hptII"
                     /number=1
     promoter        complement(8179..8856)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9047..9068
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9083..9113
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9121..9137
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9145..9161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
      241 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
      301 aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt
      361 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
      421 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
      481 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
      541 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
      601 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
      661 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
      721 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
      781 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
      841 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
      901 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
      961 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     1021 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     1081 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     1141 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     1201 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     1261 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     1321 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     1381 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     1441 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     1501 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     1561 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     1621 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     1681 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     1741 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     1801 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     1861 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     1921 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     1981 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     2041 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     2101 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     2161 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     2221 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     2281 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     2341 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     2401 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     2461 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     2521 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     2581 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     2641 ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg
     2701 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     2761 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     2821 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     2881 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     2941 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     3001 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     3061 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     3121 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     3181 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     3241 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     3301 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     3361 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     3421 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     3481 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     3541 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     3601 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     3661 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     3721 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     3781 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     3841 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     3901 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     3961 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
     4021 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
     4081 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
     4141 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
     4201 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4261 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4321 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     4381 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4441 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     4501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4561 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5101 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
     5341 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
     5401 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
     5461 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
     5521 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
     5581 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
     5641 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
     5701 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
     5761 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
     5821 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
     5881 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
     5941 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
     6001 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
     6061 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
     6121 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
     6181 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
     6241 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
     6301 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
     6361 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
     6421 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
     6481 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     6541 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     6601 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
     6661 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
     6721 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
     6781 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgaa
     6841 gatccgtcga gcttgtcgat cgacagatcc ggtcggcatc tactctattt ctttgccctc
     6901 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     6961 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     7021 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     7081 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg tggcgatcct
     7141 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     7201 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     7261 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     7321 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     7381 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     7441 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     7501 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     7561 tccgcgaccg gctgcagtta tcatcatcat catagacaca cgaaataaag taatcagatt
     7621 atcagttaaa gctatgtaat atttacacca taaccaatca attaaaaaat agatcagttt
     7681 aaagaaagat caaagctcaa aaaaataaaa agagaaaagg gtcctaacca agaaaatgaa
     7741 ggagaaaaac tagaaattta cctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     7801 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaactcccc
     7861 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     7921 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     7981 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     8041 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     8101 atctcattgc ccccccggat ctgcgaaagc tcgacggatc ttcgagagag atagatttgt
     8161 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga
     8221 ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
     8281 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     8341 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     8401 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     8461 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     8521 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     8581 agagtgtcgt gctccaccat gttatcacat caatccactt gctttgaaga cgtggttgga
     8641 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     8701 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     8761 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     8821 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     8881 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc
     8941 tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9001 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9061 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9121 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
//
