LOCUS       40924_7901        9937 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC1300intB-35SnosEX, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Binary expression vector for use in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2004) Molecular Cell Biology, Institute of 
            Biology, Leiden University, Wassenaarseweg 64, Leiden 2333 AL, The 
            Netherlands
REFERENCE   3  (bases 1 to 9937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2004) Molecular Cell Biology, Institute of Biology, Leiden 
            University, Wassenaarseweg 64, Leiden 2333 AL, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(52..304)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(322..347)
                     /note="multiple cloning site; SpeI, XhoI, EcoRI, SalI are 
                     unique"
     misc_feature    complement(349..404)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(424..768)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(828..844)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1047..1071
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2371..2997
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3434..4498
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4567..4761
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5105..5245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5431..6019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6109..6900)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7325..7349
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7427..7601)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(7652..8338)
                     /gene="hptII"
                     /number=2
     intron          complement(8339..8528)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(8529..8867)
                     /gene="hptII"
                     /number=1
     promoter        complement(8946..9623)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9814..9835
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9850..9880
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9888..9904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9912..9928
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattaattc gagctcggta cccaattctc atgtttgaca gcttatcatc ggatctagta
       61 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
      121 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      181 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
      241 tcatcgcaag accggcaaca ggattcaatt ttaagaaact ttattgccaa atgtttgaac
      301 gatctgcagc cgggcggccg cactagtccc tcgaggaatt cgtcgactgt aattgtaaat
      361 agtaattgta atgttgtttg ttgtttgttg ttgttggtaa ttgttgaagg atctcaagaa
      421 gcctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
      481 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
      541 agacgtggtt ggaacgtctt ctttttccac gatgttcctc gtgggtgggg gtccatcttt
      601 gggaccactg tcggtagagg catcttgaac gatagccttt cctttatcgc aatgatggca
      661 tttgtaggag ccaccttcct tttctactgt cctttcgatg aagtgacaga tagctgggca
      721 atggaatccg aggaggtttc ccgatattac cctttgttaa aagtctcaat agccctctgg
      781 tcttctgagg ggggatcctn tagagcctgc aggcatgcaa gcttggcact ggccgtcgtt
      841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      961 ttgcgcagcc tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc
     1021 cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     1081 aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     1141 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     1201 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     1261 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     1321 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     1381 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     1441 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     1501 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     1561 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     1621 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     1681 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     1741 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     1801 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     1861 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     1921 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     1981 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     2041 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     2101 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     2161 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     2221 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     2281 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     2341 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     2401 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     2461 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     2521 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     2581 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     2641 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     2701 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     2761 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     2821 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     2881 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     2941 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     3001 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     3061 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     3121 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     3181 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     3241 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     3301 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     3361 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag
     3421 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     3481 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     3541 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     3601 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     3661 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     3721 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     3781 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     3841 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     3901 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     3961 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     4021 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     4081 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     4141 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     4201 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     4261 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     4321 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     4381 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     4441 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     4501 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     4561 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     4621 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     4681 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     4741 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     4801 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     4861 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     4921 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     4981 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5041 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5101 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     5161 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     5221 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     5881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6061 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc
     6121 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
     6181 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
     6241 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
     6301 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
     6361 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
     6421 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
     6481 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
     6541 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
     6601 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
     6661 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
     6721 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
     6781 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
     6841 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
     6901 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
     6961 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
     7021 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
     7081 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     7141 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     7201 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     7261 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     7321 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     7381 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag
     7441 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
     7501 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa
     7561 ttcccttatc tgggaactac tcacacatta ttatggagaa actcgaagat ccgtcgagct
     7621 tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga cgagtgctgg
     7681 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     7741 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     7801 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     7861 ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca agctccggat
     7921 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     7981 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     8041 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     8101 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     8161 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     8221 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     8281 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     8341 gcagttatca tcatcatcat agacacacga aataaagtaa tcagattatc agttaaagct
     8401 atgtaatatt tacaccataa ccaatcaatt aaaaaataga tcagtttaaa gaaagatcaa
     8461 agctcaaaaa aataaaaaga gaaaagggtc ctaaccaaga aaatgaagga gaaaaactag
     8521 aaatttacct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     8581 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
     8641 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     8701 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     8761 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     8821 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
     8881 cccggatctg cgaaagctcg acggatcttc gagagagata gatttgtaga gagagactgg
     8941 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg
     9001 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     9061 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     9121 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     9181 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     9241 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     9301 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     9361 ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     9421 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     9481 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     9541 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     9601 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     9661 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
     9721 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9781 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9841 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9901 acaatttcac acaggaaaca gctatgacca tgattac
//
