LOCUS       V008969                 6225 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008969
VERSION     V008969
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG,
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus
            Reveals a Function for Non-structural Glycoproteins Cleavage by
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
   PUBMED   25933376
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA
            30333, USA
REFERENCE   3  (bases 1 to 6225)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6225)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog."; date: "2015"; volume: "11"; issue: "5"; pages: "E1004879"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2014) Viral Special Pathogens Branch, Centers for Disease
            Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30333, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      140..384
                     /label="CMV IE"
                     /note="CMV IE"
                     /regulatory_class="enhancer"
     regulatory      366..663
                     /label="chicken beta actin promoter"
                     /note="chicken beta actin promoter"
                     /regulatory_class="promoter"
     promoter        386..661
                     /label="chicken beta-actin promoter"
     intron          662..1670
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1751..3196
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Crimean-Congo hemorrhagic fever
                     virus (strain Nigeria/IbAr10200/1970). Accession#: P89522"
     polyA_signal    3314..3369
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3728..3744)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3752..3768)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3776..3806)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3821..3842)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3900..4096
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4102..4236
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4475..5063)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5237..6094)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6095..6199)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcgagctc atcgatgcat
     1741 ggttctcttg atggaaaaca agatcgaggt gaataacaaa gatgagatga acaggtggtt
     1801 tgaagagttc aaaaaaggaa atggacttgt ggacaccttc acaaactcct attccttttg
     1861 cgagagtgtt cccaatttgg acaggtttgt gtttcagatg gccagtgcca ccgatgatgc
     1921 acagaaggac tccatctacg catctgctct ggtggaggca acaaagtttt gtgcacctat
     1981 atatgagtgc gcatgggtta gctccactgg cattgtaaaa aagggacttg aatggttcga
     2041 gaaaaatgca ggaaccatta agtcctggga tgaaagttat actgagctaa aggtcgacgt
     2101 cccgaaaata gagcagctta ccggttacca acaagctgcc ttgaagtgga gaaaagacat
     2161 aggtttccgt gtcaatgcca acacagcagc tctgagcaac aaagtcctcg cagaatacaa
     2221 agtccctggt gagattgtga tgtctgtcaa agagatgctg tcagacatga ttaggagaag
     2281 gaacctgatt ctaaacaggg gtggtgatga gaacccacgt ggcccagtga gccatgagca
     2341 tgtagactgg tgcagggagt ttgtcaaagg caaatacatc atggccttca acccaccatg
     2401 gggggacatc aacaagtcag gccgttcagg aatagcactt gttgcaacag gccttgctaa
     2461 gcttgcagag actgaaggaa agggaatatt tgatgaagcc aaaaagactg tggaggccct
     2521 caacgggtat ctggacaagc ataaggacga agttgataga gcaagcgccg acagcatgat
     2581 aacaaacctt cttaagcata ttgccaaggc acaggagctc tataaaaatt catctgcact
     2641 tcgtgcacaa agcgcacaga ttgacactgc tttcagctca tactattggc tttacaaggc
     2701 tggcgtgact cctgaaacct tcccgacggt gtcacagttc ctctttgagc tagggaaaca
     2761 gccaagaggt accaagaaaa tgaagaaggc tcttctgagc accccaatga agtgggggaa
     2821 gaagctttat gagctctttg ccgatgattc tttccagcag aacaggattt acatgcatcc
     2881 tgccgtgctt acagctggta gaatcagtga aatgggagtc tgctttggga caatccctgt
     2941 ggccaatcct gatgatgctg cccaaggatc tggacacact aagtctattc tcaacctccg
     3001 taccaacact gagaccaata atccgtgtgc caaaaccatc gtcaagctat ttgaagttca
     3061 aaaaacaggg ttcaacattc aggacatgga catagtggcc tctgagcact tgctacacca
     3121 atcccttgtt ggcaagcaat ccccattcca gaacgcctac aacgtcaagg gcaatgccac
     3181 cagtgctaac atcatttaaa atacaaactg ctctgtactc aacttccggg catgctcgag
     3241 ctagcagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     3301 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     3361 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     3421 ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata
     3481 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     3541 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     3601 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     3661 agtcatagct gtccctcttc tcttatgaag atccctcgac ctgcagccca agcttggcgt
     3721 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     3901 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3961 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4021 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4081 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
     4141 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4201 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
     4261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     4681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5221 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6061 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctg
//
