LOCUS       40924_7731        8600 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC-(loxP2-attB2-SA(2)), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH.
  TITLE     Plug-and-Play Genetic Access to Drosophila Cell Types using 
            Exchangeable Exon Cassettes
  JOURNAL   Cell Rep 10 (8), 1410-1421 (2015)
  PUBMED    25732830
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Diao F, Luan H, White BH.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Laboratory of Molecular Biology, NIMH, NIH, 
            35 Convent Dr, Bethesda, MD 20850, USA
REFERENCE   3  (bases 1 to 8600)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep"; 
            date: "2015"; volume: "10"; issue: "8"; pages: "1410-1421"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2015) Laboratory of Molecular Biology, NIMH, NIH, 35 Convent
            Dr, Bethesda, MD 20850, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Version 11
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..242
                     /label=Carl_P5' P-element
                     /note="Carl_P5' P-element"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3069..3102
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3218..3287
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    3315..3414
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     protein_bind    complement(3635..3704)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_recomb     3820..3853
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(3820..3853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            3876..8004
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8015..8600)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgagat aacttcgtat aatgtatgct atacgaagtt atcaattggc ttattgggat
     3121 ttcaatttaa gatgctgcta aataaagtta gtcacttacg gactggggag gcaccccgtc
     3181 atcactcgcc agaaatgcaa ttgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
     3241 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
     3301 tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat
     3361 atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtcggg
     3421 aggttccggt ggaagcggag gtagcggcgg atccggaggt agcggtggaa gcggaggttc
     3481 cggcaggtaa gttattgaac aatggcatca aatgccttca tcatcactac cctttagccc
     3541 ttaagacccc acaatgacct tacccactca gagaaaaaag taaatatgaa agcccatttg
     3601 aacttctaag cttgtcgacc atcatgatgg accagatggg tgaggtggag tacgcgcccg
     3661 gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagaccgtg acctacatca
     3721 tacaataaat attcacaccc ctaaaaaacg aagctgcatt tgcaaattgc atacaattat
     3781 gccagagtaa aaattataat tgttattatt taaatttgaa taacttcgta taatgtatgc
     3841 tatacgaagt tatagatcca ctagaaggcc taattctagt atgtatgtaa gttaataaaa
     3901 cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca ctggatatca
     3961 ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga agtaccaggt
     4021 tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc
     4081 ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc
     4141 accgtttgta gcgttaccta gcgtcaatgt ccgccttcag ttgcactttg tcagcggttt
     4201 cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact
     4261 cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt
     4321 gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt
     4381 aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg
     4441 tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg
     4501 cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac
     4561 acgaaacaga ttattctggt agctgtgctc gctatataag acaattttta agatcatatc
     4621 atgatcaaga catctaaagg cattcatttt cgactacatt cttttttaca aaaaatataa
     4681 caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac
     4741 ttcgtaggat acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt
     4801 gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg
     4861 gcttcgggca ggccaaaaac tacggcacgc tcctgccacc cagtccgccg gaggactccg
     4921 gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct
     4981 ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac
     5041 tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt
     5101 ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg
     5161 aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg
     5221 tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt
     5281 atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc
     5341 caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca
     5401 cctgattttc caggccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc
     5461 ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg
     5521 tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc
     5581 cgaggcacta accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc
     5641 atttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt
     5701 catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct
     5761 gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc
     5821 ctagtgagtt cgatgtgttt attaagggta tctagcatta cattacatct caactcctat
     5881 ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt
     5941 tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca
     6001 attttgctat tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatttgg
     6061 agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt
     6121 tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag
     6181 tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca
     6241 attcttggaa acaaattcgc tagattttag ttagaattgc ctgattccac acccttctta
     6301 gtttttttca atgagatgta tagtttatag ttttgcagaa aataaataaa tttcatttaa
     6361 ctcgcgaaca tgttgaagat atgaatatta atgagatgcg agtaacattt taatttgcag
     6421 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggcgtg
     6481 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     6541 gccacgataa atgtaagtct tgtttagaat acatttgcat attaataatt tactaacttt
     6601 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     6661 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattacc
     6721 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     6781 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     6841 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     6901 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     6961 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     7021 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     7081 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     7141 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     7201 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     7261 cttgtttttt tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag
     7321 ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt
     7381 ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac
     7441 acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt
     7501 ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt
     7561 ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc
     7621 gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga
     7681 gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc
     7741 tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac
     7801 tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa
     7861 gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag
     7921 tgttgtttat atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa
     7981 atgcttggat ttcactggaa ctagaattaa ttcggctgct gctctaaacg acgcatttcg
     8041 tactccaaag tacgaatttt ttccctcaag ctcttatttt cattaaacaa tgaacaggac
     8101 ctaacgcaca gtcacgttat tgtttacata aatgattttt tttactattc aaacttactc
     8161 tgtttgtgta ctcccactgg tatagccttc ttttatcttt tctggttcag gctctatcac
     8221 tttactaggt acggcatctg cgttgagtcg cctcctttta aatgtctgac cttttgcagg
     8281 tgcagccttc cactgcgaat cattaaagtg ggtatcacaa atttgggagt tttcaccaag
     8341 gctgcaccca aggctctgct cccacaattt tctcttaata gcacacttcg gcacgtgaat
     8401 taattttact ccagtcacag ctttgcagca aaatttgcaa tatttcattt ttttttattc
     8461 cacgtaaggg ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca
     8521 agcaaacgtg cactgaattt aagtgtatac ttcggtaagc ttcggctatc gacgggacca
     8581 ccttatgtta tttcatcatg
//
