LOCUS       40924_7716       11728 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC-(lox2-attB2-SA-Hsp70)3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH.
  TITLE     Plug-and-Play Genetic Access to Drosophila Cell Types using 
            Exchangeable Exon Cassettes
  JOURNAL   Cell Rep 10 (8), 1410-1421 (2015)
  PUBMED    25732830
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   Diao F, Luan H, White BH.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Laboratory of Molecular Biology, NIMH, NIH, 
            35 Convent Dr, Bethesda, MD 20850, USA
REFERENCE   3  (bases 1 to 11728)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11728)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep"; 
            date: "2015"; volume: "10"; issue: "8"; pages: "1410-1421"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2015) Laboratory of Molecular Biology, NIMH, NIH, 35 Convent
            Dr, Bethesda, MD 20850, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Version 11
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..242
                     /label=Carl_P5' P-element
                     /note="Carl_P5' P-element"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3069..3102
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    3103..3202
                     /label=spacer sequence between lox2272 and attB
                     /note="spacer sequence between lox2272 and attB"
     protein_bind    3218..3287
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    3315..3414
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     misc_feature    3448..3467
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    3468..3958
                     /label=Hsp70 ployA
                     /note="Hsp70 ployA"
     misc_recomb     complement(4125..4224)
                     /label=attB
                     /note="attB"
     protein_bind    4140..4209
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    4225..4324
                     /label=spacer sequence between lox2272 and attB
                     /note="spacer sequence between lox2272 and attB"
     misc_recomb     4325..4358
                     /label=lox2272
                     /note="lox2272"
     protein_bind    complement(4325..4358)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     misc_feature    4359..4378
                     /label=spacer sequence between between lox2272 and loxN
                     /note="spacer sequence between between lox2272 and loxN"
     protein_bind    4379..4412
                     /label=loxN
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     misc_feature    4413..4512
                     /label=spacer sequence between loxN and attB
                     /note="spacer sequence between loxN and attB"
     misc_recomb     4513..4612
                     /label=attB
                     /note="attB"
     protein_bind    4528..4597
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    4625..4724
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     misc_feature    4760..4779
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    4780..5270
                     /label=Hsp70 polyA
                     /note="Hsp70 polyA"
     misc_recomb     complement(5435..5534)
                     /label=attB
                     /note="attB"
     protein_bind    5450..5519
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    5535..5634
                     /label=spacer sequence between attB and loxN
                     /note="spacer sequence between attB and loxN"
     misc_recomb     5635..5668
                     /label=loxN
                     /note="loxN"
     protein_bind    complement(5635..5668)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     misc_feature    5669..5688
                     /label=spacer sequence between loxN and loxP
                     /note="spacer sequence between loxN and loxP"
     protein_bind    5689..5722
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5723..5822
                     /label=spacer sequence between loxP and attB
                     /note="spacer sequence between loxP and attB"
     misc_recomb     5823..5922
                     /label=attB
                     /note="attB"
     protein_bind    5838..5907
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    5935..6034
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     misc_feature    6069..6088
                     /label=MCS
                     /note="MCS"
     misc_feature    6089..6579
                     /label=Hsp70 ployA
                     /note="Hsp70 ployA"
     protein_bind    complement(6760..6829)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    6845..6944
                     /label=spacer sequence between attB and loxP
                     /note="spacer sequence between attB and loxP"
     misc_feature    6945..6978
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(6945..6978)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7001..11127
                     /label=white gene marker
                     /note="white gene marker"
     CDS             join(7483..7554,7949..8222,8297..8951,9013..9328,9549..9680,
                     9751..10365)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    complement(11143..11728)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgagat aacttcgtat aaagtatcct atacgaagtt atcaattggc ttattgggat
     3121 ttcaatttaa gatgctgcta aataaagtta gtcacttacg gactggggag gcaccccgtc
     3181 atcactcgcc agaaatgcaa ttgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
     3241 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
     3301 tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat
     3361 atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtggga
     3421 ggttccggtg gaagcggagg tagcggcacc ggtgcatgcg cggccgcaat gaatcgtaga
     3481 tactgaaaaa ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc aatttctttc
     3541 atttatacat cgttttgcct tcttttatgt aactatactc ctctaagttt caatcttggc
     3601 catgtaacct ctgatctata gaatttttta aatgactaga attaatgccc atcttttttt
     3661 tggacctaaa ttcttcatga aaatatatta cgagggctta ttcagaagct tatcgatacc
     3721 gtcgactaaa gccaaataga aattattcag ttctggctta agtttttaaa agtgatatta
     3781 tttatttggt tgtaaccaac caaaagaatg taaataacta atacataatt atgttagttt
     3841 taagttagca acaaattgat tttagctata ttagctactt ggttaataaa tagaatatat
     3901 ttatttaaag ataattcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttgg
     3961 aggtagcggt ggaagcggag gttccggcga ggtaagttat tgaacaatgg catcaaatgc
     4021 cttcatcatc actacccttt agcccttaag accccacaat gaccttaccc actcagagaa
     4081 aaaagtaaat atgaaagccc atttgaactt ctaagcttgt cgaccatcat gatggaccag
     4141 atgggtgagg tggagtacgc gcccggggag cccaagggca cgccctggca cccgcaccgc
     4201 ggcttcgaga ccgtgaccta catcatacaa taaatattca cacccctaaa aaacgaagct
     4261 gcatttgcaa attgcataca attatgccag agtaaaaatt ataattgtta ttatttaaat
     4321 ttgaataact tcgtataaag tatcctatac gaagttatgt cgactgcaga atttcgagat
     4381 aacttcgtat aaggtatact atacgaagtt atgcatgacg cacagctgta ggactattat
     4441 taaaattctt atttcggggg agagctgtaa ttggtggatg atgagtcctt aaaaaaatcg
     4501 ttgtaccaaa gggatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
     4561 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tggtcgactc
     4621 tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat atactaatta
     4681 ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtggcg gaggttccgg
     4741 tggaagcgga ggtagcggca ctagtacgcg tggccggcca atgaatcgta gatactgaaa
     4801 aaccccgcaa gttcacttca actgtgcatc gtgcaccatc tcaatttctt tcatttatac
     4861 atcgttttgc cttcttttat gtaactatac tcctctaagt ttcaatcttg gccatgtaac
     4921 ctctgatcta tagaattttt taaatgacta gaattaatgc ccatcttttt tttggaccta
     4981 aattcttcat gaaaatatat tacgagggct tattcagaag cttatcgata ccgtcgacta
     5041 aagccaaata gaaattattc agttctggct taagttttta aaagtgatat tatttatttg
     5101 gttgtaacca accaaaagaa tgtaaataac taatacataa ttatgttagt tttaagttag
     5161 caacaaattg attttagcta tattagctac ttggttaata aatagaatat atttatttaa
     5221 agataattcg tttttattgt cagggagtga gtttgcttaa aaactcgttt ggaggtagcg
     5281 gtggaagcgg aggttccgga ggtaagttat tgaacaatgg catcaaatgc cttcatcatc
     5341 actacccttt agcccttaag accccacaat gaccttaccc actcagagaa aaaagtaaat
     5401 atgaaagccc atttgaactt ctaagcttgt cgaccatcat gatggaccag atgggtgagg
     5461 tggagtacgc gcccggggag cccaagggca cgccctggca cccgcaccgc ggcttcgaga
     5521 ccgtgaccta catccataga ttttgttaat cggcttttgg tatgcacaaa atgattggat
     5581 attatgccaa cttgttttct attcactgta gattagctta caaaaaaata tcccataact
     5641 tcgtataagg tatactatac gaagttatgt cgactgcaga atttcgagat aacttcgtat
     5701 aatgtatgct atacgaagtt ataatcccct gtgctcagtg gggtatttac tattaaacca
     5761 cacatgctga gcctggctta ttggcggctg aatcacccgg agatacggca aatttggagc
     5821 ttgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc
     5881 ccgggcgcgt actccacctc acccatctgg tccatcatga tggtcgactc tagaagtcga
     5941 tccaacatgg cgacttgtcc catccccggc atgtttaaat atactaatta ttcttgaact
     6001 aattttaatc aaccgattta tctctcttcc gcaggtcggg aggttccggt ggaagcggag
     6061 gtagcggcga attccgtacg ggcgcgccaa tgaatcgtag atactgaaaa accccgcaag
     6121 ttcacttcaa ctgtgcatcg tgcaccatct caatttcttt catttataca tcgttttgcc
     6181 ttcttttatg taactatact cctctaagtt tcaatcttgg ccatgtaacc tctgatctat
     6241 agaatttttt aaatgactag aattaatgcc catctttttt ttggacctaa attcttcatg
     6301 aaaatatatt acgagggctt attcagaagc ttatcgatac cgtcgactaa agccaaatag
     6361 aaattattca gttctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa
     6421 ccaaaagaat gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga
     6481 ttttagctat attagctact tggttaataa atagaatata tttatttaaa gataattcgt
     6541 ttttattgtc agggagtgag tttgcttaaa aactcgtttg gaggtagcgg tggaagcgga
     6601 ggttccggca ggtaagttat tgaacaatgg catcaaatgc cttcatcatc actacccttt
     6661 agcccttaag accccacaat gaccttaccc actcagagaa aaaagtaaat atgaaagccc
     6721 atttgaactt ctaagcttgt cgaccatcat gatggaccag atgggtgagg tggagtacgc
     6781 gcccggggag cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta
     6841 catcttcaat tttaataccc aatggagtaa tgcaaattgt tagcagattt ttcgactttc
     6901 cccctgccag aaacaaatca aatgcaatta aaaacctttg cgccataact tcgtataatg
     6961 tatgctatac gaagttatgg atccactaga aggccttagt atgtatgtaa gttaataaaa
     7021 cccttttttg gagaatgtag atttaaaaaa acatattttt tttttatttt ttactgcact
     7081 ggacatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgaag
     7141 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     7201 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     7261 ctttcgccac cgtttgtagc gttacctagc gtcaatgtcc gccttcagtt gcactttgtc
     7321 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     7381 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     7441 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     7501 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     7561 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     7621 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa
     7681 atagtgacac gaaacagatt attctggtag ctgtgctcgc tatataagac aatttttaag
     7741 atcatatcat gatcaagaca tctaaaggca ttcattttcg actacattct tttttacaaa
     7801 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     7861 aaacctactt cgtaggatac ttcgttttgt tcggggttag atgagcataa cgcttgtagt
     7921 tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat
     7981 taaccagggc ttcgggcagg ccaaaaacta cggcacgctc ctgccaccca gtccgccgga
     8041 ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat
     8101 ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac
     8161 acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa
     8221 cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta
     8281 ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc
     8341 agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc
     8401 atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag
     8461 atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc
     8521 agggaacacc tgattttcca ggccatggtg cggatgccac gacatctgac ctatcggcag
     8581 cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg
     8641 atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca
     8701 ttcgcctccg aggcactaac cgatccgccg cttctgatct gcgatgagcc cacctccgga
     8761 ctggactcat ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc
     8821 aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag
     8881 atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac
     8941 ttcttttcct agtgagttcg atgtgtttat taagggtatc tagcattaca ttacatctca
     9001 actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt
     9061 acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat
     9121 atgcgacaat tttgctatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa
     9181 aaatttggag aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt
     9241 catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct
     9301 cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa
     9361 cgcttacaat tcttggaaac aaattcgcta gattttagtt agaattgcct gattccacac
     9421 ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaaa taaataaatt
     9481 tcatttaact cgcgaacatg ttgaagatat gaatattaat gagatgcgag taacatttta
     9541 atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag
     9601 tgggcgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa
     9661 acgtctttgc cacgataaat gtaagtcttg tttagaatac atttgcatat taataattta
     9721 ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg
     9781 agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc
     9841 gaattaccgc tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc
     9901 ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc
     9961 aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg
    10021 ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac
    10081 tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc
    10141 aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca
    10201 tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc
    10261 gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg
    10321 ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga
    10381 aataactgct tgtttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc
    10441 tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac
    10501 aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag
    10561 acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga
    10621 tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca
    10681 caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc
    10741 ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat
    10801 ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat
    10861 ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat
    10921 attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg
    10981 aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg
    11041 cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa
    11101 tttagaaaat gcttggattt cactggaact agaattaatt cggctgctgc tctaaacgac
    11161 gcatttcgta ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg
    11221 aacaggacct aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa
    11281 acttactctg tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc
    11341 tctatcactt tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct
    11401 tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt
    11461 tcaccaaggc tgcacccaag gctctgctcc cacaattttc tcttaatagc acacttcggc
    11521 acgtgaatta attttactcc agtcacagct ttgcagcaaa atttgcaata tttcattttt
    11581 ttttattcca cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca caacctttcc
    11641 tctcaacaag caaacgtgca ctgaatttaa gtgtatactt cggtaagctt cggctatcga
    11701 cgggaccacc ttatgttatt tcatcatg
//
