LOCUS       V008984                 7383 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008984
VERSION     V008984
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O,
            Missiakas DM.
  TITLE     Staphylococcus aureus virulence genes identified by bursa aurealis
            mutagenesis and nematode killing
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12312-12317 (2004)
   PUBMED   15304642
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Molecular Genetics and Cell Biology,
            Committee on Microbiology, University of Chicago, 920 E. 58th St.,
            Chicago, IL 60637, USA
REFERENCE   3  (bases 1 to 7383)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2004) Molecular Genetics and Cell Biology,
            Committee on Microbiology, University of Chicago, 920 E. 58th St.,
            Chicago, IL 60637, USA
REFERENCE   4  (bases 1 to 7383)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2005) Molecular Genetics and Cell Biology,
            Committee on Microbiology, University of Chicago, 920 E. 58th St.,
            Chicago, IL 60637, USA
REFERENCE   5  (bases 1 to 7383)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "33"; pages:
            "12312-12317"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2004) Molecular Genetics and Cell Biology, Committee on
            Microbiology, University of Chicago, 920 E. 58th St., Chicago, IL
            60637, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (04-NOV-2004) Molecular Genetics and Cell Biology, Committee on
            Microbiology, University of Chicago, 920 E. 58th St., Chicago, IL
            60637, USA"
            SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
            (05-OCT-2005) Molecular Genetics and Cell Biology, Committee on
            Microbiology, University of Chicago, 920 E. 58th St., Chicago, IL
            60637, USA"
            SGRef: number: 5; type: "Journal Article"
            On Oct 5, 2005 this sequence version replaced AY672109.2.
FEATURES             Location/Qualifiers
     source          1..7383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             841..1008
                     /codon_start=1
                     /product="cop-6"
                     /label="cop-6"
                     /protein_id="ABA61860.1"
                     /translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILIK
                     KYAKEEYGAF"
     CDS             1089..1688
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein"
                     /label="repC"
                     /protein_id="AAU04870.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            1089..1688
                     /gene="repC"
                     /label="repC"
     rep_origin      1219..1418
                     /label="from pE194"
                     /note="from pE194"
     CDS             3034..3681
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(4515..5249)
                     /gene="ermB"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis (strain ATCC 700802 / V583). Accession#: P20173"
     primer_bind     5805..5821
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(5958..6346)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(6577..7290)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
ORIGIN
        1 aagcttatcg atttcgaacc cggggtaccg aattcctcga gtctagagga gcatgcgacg
       61 tcgcggaatt gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca
      121 agaattaaaa aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta
      181 tcacgaaaaa agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca
      241 gttgaatgta tcagagaaat ttcaagatag tacagtgact ttatatcgtg ctgcgagggc
      301 gaatttccct gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc
      361 caaattcgag cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga
      421 tagaaagcgt gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga
      481 gaaaactttt tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag
      541 cgacagctat taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac
      601 acgcaaaaag gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga
      661 ggtctttttt tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg
      721 acctcccccc taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt
      781 aatgttaaaa ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg
      841 atggttgtag atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa
      901 gaaaatgaga tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc
      961 ggtattctaa taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat
     1021 agacagcact ggcatgctgc ctatctatga ctaaattttg ttaaatgtat tagcaccgtt
     1081 attatatcat gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac
     1141 gtaattggac atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa
     1201 aagagttaca cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag
     1261 ataggatgaa aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg
     1321 aacagataaa aataattaca gaagaattga atgcgactat tccgcagatt gcaggaagtg
     1381 tgaaaggtct tgtgagatat atgcttcaca tggacgatcc taataaattt aaatatcaaa
     1441 aagaagatat gatagtttat ggcggtgtag atgttgatga attattaaag aaaacaacaa
     1501 cagatagata taaattaatt aaagaaatga ttgagtttat tgatgaacaa ggaatcgtag
     1561 aatttaagag tttaatggat tatgcaatga agtttaaatt tgatgattgg ttcccgcttt
     1621 tatgtgataa ctcggcgtat gttattcaag aatatataaa atcaaatcgg tataaatctg
     1681 accgatagat tttgaattta ggtgtcacaa gacactcttt tttcgcacca gcgaaaactg
     1741 gtttaagccg actgcgcaaa agacataatc gattcacaaa aaataggcac acgaaaaaca
     1801 agttaaggga tgcagtttat gcatccctta acttacttat taaataattt atagctattg
     1861 aaaagagata agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt
     1921 aaggaattgt taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc
     1981 ataacgaaat aattatactt ttgtttatct ttgtgtgata ttcttgattt ttttctactt
     2041 aatctgataa gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata
     2101 cttaatatag aaatatcaac ttctgccatt aaaagtaatg ccaatgagcg ttttgtattt
     2161 aataatcttt tagcaaaccc gtattccacg attaaataaa tctcattagc tatactatca
     2221 aaaacaattt tgcgtattat atccgtactt atgttataag gtatattacc atatatttta
     2281 taggattggt ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta
     2341 tcgtgatctt ccttcaggtt atgaccatct gtgccagttc gtaatgtctg gtcaactttc
     2401 cgactctgag aaacttctgg aatcgctaga gaatttctgg aatgggattc aggagtggac
     2461 agaacgacac ggatatatag tggatgtgtc aaaacgcata ccattttgaa cgatgacctc
     2521 taataattgt taatcatgtt ggttacgtat ttattaactt ctcctagtat tagtaattat
     2581 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt
     2641 aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat
     2701 tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg caatagttac
     2761 ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc tttaaaaaaa
     2821 cacaaaagac cacatttttt aatgtggtct ttattcttca actaaagcac ccattagttc
     2881 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
     2941 attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat
     3001 tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac
     3061 aattggaaga gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata
     3121 accacagaaa ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt
     3181 taccctgcat ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact
     3241 ggttacaata gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt
     3301 tttgatggtg tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa
     3361 gagttttatg atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt
     3421 cccaaaacac ctatacctga aaatgctttt tctctttcta ttattccatg gacttcattt
     3481 actgggttta acttaaatat caataataat agtaattacc ttctacccat tattacagca
     3541 ggaaaattca ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat
     3601 tctgtttgtg atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat
     3661 aggcctaatg actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt
     3721 tttctaacga tacattaata ggtacgaaaa agcaactttt tttgcgctta aaaccagtca
     3781 taccaataac ttaagggtaa ctagcctcgc cggaaagagc gaaaatgcct cacatttgtg
     3841 ccacctaaaa aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa
     3901 atcagctgga ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa
     3961 tgatgatatt gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat
     4021 tgattacaac gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac
     4081 gaggaaagat gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga
     4141 gcaactggat ccagactgga cggtctagta acaggttggc tgataagtcc ccggtctcta
     4201 gacgcgtata actatatagg aactatgaaa aaggaacgga ttttggtact tgatttactt
     4261 aaaaaacatt tagaaaaagc aggaaaatga aatggtacca taaacggtcg tttatggtac
     4321 cattcaaatt tatccttatt gtacaaaata acagcgaaat ttttaaatct attccttatc
     4381 gatacaaatt ccccgtaggc gctagggacc tctttagctc cttggaagct gtcagtagta
     4441 tacctaataa tttatctaga ttccctttag taacgtgtaa ctttccaaat ttacaaaagc
     4501 gactcataga attatttcct cccgttaaat aatagataac tattaaaaat agacaatact
     4561 tgctcataag taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac
     4621 tgatttttag taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta
     4681 tatagcttcc aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca
     4741 ctgtttactt ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc
     4801 gagacttgag tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag
     4861 aatccttctt caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta
     4921 ataatttgtg tgcttaaatg gtaaggaata ttcccaacaa ttttatacct ctgtttgtta
     4981 gggaattgaa actgtagaat atcttggtga attaaagtga cacgaatgtt cagttttaat
     5041 ttttctgacg ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt
     5101 attttagcca gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg
     5161 gtttctttta aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag
     5221 ttttgagaat attttatatt tttgttcatg taatcactcc tgaagtgatt atatctataa
     5281 ataaatacag aagttaaacg atttgtttgt aattttagtt atctgtttaa aaagtcataa
     5341 gattagtcac tggtaggaat taatctaacg tatttattta tctgcgtaat cactgttttt
     5401 agtctgtttc aaaacagtag atgttttatc tacattacgc atttggaata ccaacatgac
     5461 gaatccctcc ttcttaatta caaattttta gcatctaatt taacttcaat tcctattata
     5521 caaaatttta agataatgca ctatcaacac actcttaagt ttgcttctaa gtcttatttc
     5581 cataacttta gggttaacca tacgcaagac caatcactct cggacaatac tcatgatttt
     5641 aatgataaaa tccatgttaa acccatagat aagaaataca cctgcaataa ccgttacctg
     5701 tttgtgccaa tttaaaaatg caccactttt ttataattaa aacggttgaa aactgtacca
     5761 ctaataactc acaatagacg cgtcctcggt accgggcccc ccctcgaggt cgacggtatc
     5821 gataagcttg atatcgaatt cctgcagccc ttaattaacc ccgaaaagtg ccacctgacg
     5881 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5941 ttcgtcttca agaattctgt cagccgttaa gtgttcctgt gtcactgaaa attgctttga
     6001 gaggctctaa gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac
     6061 cttaaaagct ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga
     6121 ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt
     6181 gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta
     6241 gttcgttaaa catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag
     6301 agcttagtac gtactatcaa caggttgaac tgctgatctt cagatcctct acgccggacg
     6361 catcgtgggg atctggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     6421 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6481 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6541 ttgttccagg gcgcgccctg caggtctgga catttatttg tatagttcat ccatgccatg
     6601 tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct tttcgttggg
     6661 atctttcgaa agggcagatt gtgtggacag gtaatggttg tctggtaaaa ggacagggcc
     6721 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc catcttcaat
     6781 gttgtgtcta attttgaagt taactttgat tccattcttt tgtttgcctg ccatgatgta
     6841 tacattgtgt gagttatagt tgtattccaa tttgtgtcca agaatgtttc catcttcttt
     6901 aaaatcaata ccttttaact cgattctatt aacaagggta tcaccttcaa acttgacttc
     6961 agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata gttctttcct gtacataacc
     7021 ttcgggcatg gcactcttga aaaagtcatg ctgtttcata tgatctgggt atctcgcaaa
     7081 gcattgaaca ccataaccga aagtagtgac aagtgttggc catggaacag gtagttttcc
     7141 agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt caccctctcc
     7201 actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac
     7261 tccagtgaaa agttcttctc ctttactcat atgtatatct ccggcgcgcc gcgacgccat
     7321 ctatgtgtct agagaccggg gacttatcag ccaacctgtt actagagggc ccaattcgga
     7381 tcc
//
