LOCUS       40924_7671        8700 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-tg-n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8700)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2488
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2795..3046
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3047..3213
                     /label=kan
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3214..4005
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4122..4226
                     /label=AmpR promoter
     CDS             4227..5084
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      5093..5458
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5112..5456
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5459..5475
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5484..6200
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    6207..6331
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6763..7065)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7413..8069)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(8070..8172)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8350..8474)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      8539..8694
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
     2221 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaaggg atccatgtcc gtggagaggc ttgaccaatc
     2521 aagtgacaga atcagggaga gtagaagact aatgctggag acagaagagg ttggcatctc
     2581 aattgtccaa gatttgagtc agcaacgcca aaccctactt catgcgcaca acaagcttca
     2641 tggtgtggat gacgccattg acaagagcaa gaaggtgttg acggctatgt caaggagaat
     2701 gactaggaac aaatggatca ttacatcggt aatcgtggct ctcgttctcg ccatcatctt
     2761 gatcatctca tacaagcttt ctcattaagg accggtcgtt caaacatttg gcaataaagt
     2821 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2881 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2941 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3001 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatccgat gataagctgt
     3061 caaacatgag agctcccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     3121 tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag gggatcaagc
     3181 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3241 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3301 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3361 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     3421 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3481 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3541 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     3601 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     3661 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     3721 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     3781 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3841 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3901 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     3961 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaca cgtgttaatt
     4021 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4081 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4141 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4201 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4261 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4321 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4381 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4441 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4501 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4561 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4621 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4681 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4741 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4801 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4861 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4921 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4981 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5041 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaagtt aacagaagac
     5101 cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     5161 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa
     5221 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc
     5281 aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct
     5341 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     5401 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg
     5461 aggtcgacgg tatcgataag cttatggtga gcaagggcga ggagctgttc accggggtgg
     5521 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     5581 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     5641 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg
     5701 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     5761 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     5821 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     5881 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     5941 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     6001 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     6061 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     6121 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     6181 gcatggacga gctgtacaag catatgacaa gtttgtacaa aaaagctgaa cgagaaacgt
     6241 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
     6301 actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt attagtgacc
     6361 tgtagtcgac cgacagcctt ccaaatgttc ttcgggtgat gctgccaact tagtcgaccg
     6421 acagccttcc aaatgttctt ctcaaacgga atcgtcgtat ccagcctact cgctattgtc
     6481 ctcaatgccg tattaaatca taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg
     6541 tgtgacaaaa taaaaacatc tacctattca tatacgctag tgtcatagtc ctgaaaatca
     6601 tctgcatcaa gaacaatttc acaactctta tacttttctc ttacaagtcg ttcggcttca
     6661 tctggatttt cagcctctat acttactaaa cgtgataaag tttctgtaat ttctactgta
     6721 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
     6781 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
     6841 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
     6901 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
     6961 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
     7021 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
     7081 tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
     7141 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
     7201 ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
     7261 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac
     7321 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
     7381 gcttttagta agccggatcc acgcggcgtt tacgccccgc cctgccactc atcgcagtac
     7441 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     7501 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     7561 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     7621 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     7681 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     7741 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     7801 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     7861 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     7921 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     7981 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8041 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8101 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8161 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8221 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8281 caaagtgcgt cgggtgatgc tgccaactta gtcgactaca ggtcactaat accatctaag
     8341 tagttgattc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     8401 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     8461 ttgtacaaag tggtctgatc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
     8521 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
     8581 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     8641 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgaagcgct
//
