LOCUS       40924_7666        8728 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-tg-c, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8728)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2488
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2795..3046
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3047..3213
                     /label=kan
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3214..4005
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4122..4226
                     /label=AmpR promoter
     CDS             4227..5084
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      5093..5458
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5112..5456
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5459..5475
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5509..5633
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6065..6367)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(6715..7371)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7372..7474)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(7652..7776)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7787..8503
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      8567..8722
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
     2221 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaaggg atccatgtcc gtggagaggc ttgaccaatc
     2521 aagtgacaga atcagggaga gtagaagact aatgctggag acagaagagg ttggcatctc
     2581 aattgtccaa gatttgagtc agcaacgcca aaccctactt catgcgcaca acaagcttca
     2641 tggtgtggat gacgccattg acaagagcaa gaaggtgttg acggctatgt caaggagaat
     2701 gactaggaac aaatggatca ttacatcggt aatcgtggct ctcgttctcg ccatcatctt
     2761 gatcatctca tacaagcttt ctcattaagg accggtcgtt caaacatttg gcaataaagt
     2821 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2881 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2941 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3001 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatccgat gataagctgt
     3061 caaacatgag agctcccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     3121 tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag gggatcaagc
     3181 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3241 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3301 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3361 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     3421 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3481 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3541 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     3601 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     3661 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     3721 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     3781 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3841 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3901 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     3961 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaca cgtgttaatt
     4021 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4081 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4141 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4201 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4261 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4321 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4381 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4441 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4501 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4561 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4621 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4681 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4741 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4801 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4861 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4921 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4981 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5041 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaagtt aacagaagac
     5101 cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     5161 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa
     5221 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc
     5281 aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct
     5341 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     5401 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg
     5461 aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccatcaac aagtttgtac
     5521 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     5581 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atgaatcaac
     5641 tacttagatg gtattagtga cctgtagtcg accgacagcc ttccaaatgt tcttcgggtg
     5701 atgctgccaa cttagtcgac cgacagcctt ccaaatgttc ttctcaaacg gaatcgtcgt
     5761 atccagccta ctcgctattg tcctcaatgc cgtattaaat cataaaaaga aataagaaaa
     5821 agaggtgcga gcctcttttt tgtgtgacaa aataaaaaca tctacctatt catatacgct
     5881 agtgtcatag tcctgaaaat catctgcatc aagaacaatt tcacaactct tatacttttc
     5941 tcttacaagt cgttcggctt catctggatt ttcagcctct atacttacta aacgtgataa
     6001 agtttctgta atttctactg tatcgacctg cagactggct gtgtataagg gagcctgaca
     6061 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     6121 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
     6181 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg
     6241 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
     6301 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
     6361 actgcatttc accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg
     6421 gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg
     6481 gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga
     6541 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct
     6601 ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg
     6661 caaatacgca tactgttatc tggcttttag taagccggat ccacgcggcg tttacgcccc
     6721 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     6781 atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     6841 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     6901 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     6961 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     7021 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     7081 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     7141 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     7201 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     7261 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     7321 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     7381 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     7441 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     7501 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     7561 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tagtcgacta
     7621 caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt gtgttttaca
     7681 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     7741 ttacgtttct cgttcagctt tcttgtacaa agtggttgcc acggccatgg tgagcaaggg
     7801 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     7861 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     7921 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     7981 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     8041 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     8101 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     8161 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     8221 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     8281 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     8341 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     8401 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     8461 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaatcta gagtccgcaa
     8521 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     8581 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     8641 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     8701 tcctaaaacc aaaatccagt gaagcgct
//
