LOCUS       V008989                 9292 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008989
VERSION     V008989
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A,
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM,
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
   PUBMED   24905498
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y.,
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD,
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 9292)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        1406..1751
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2632
                     /gene="PIP2-1"
                     /label="Aquaporin PIP2-1"
                     /note="Aquaporin PIP2-1 from Arabidopsis thaliana.
                     Accession#: P43286"
     CDS             2648..3364
                     /label="ECFP"
                     /note="enhanced CFP"
     terminator      3386..3638
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3639..3805
                     /label="kan"
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3806..4597
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        4714..4818
                     /label="AmpR promoter"
     CDS             4819..5676
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      5685..6050
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5704..6048
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6051..6067
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             6076..6792
                     /label="EYFP"
                     /note="enhanced YFP"
     protein_bind    6799..6923
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7355..7657)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(8005..8661)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(8662..8764)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8942..9066)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      9131..9286
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggcaaag gatgtggaag ccgttcccgg
     1801 agaaggattt cagacaagag actatcaaga tccgccacca gctccgttta ttgatggagc
     1861 ggagctaaag aagtggtctt tctacagagc agttatcgca gagttcgtag ccactctcct
     1921 cttcttatac atcaccgttt tgacagtcat cggttacaag attcagtccg atactgatgc
     1981 cggtggcgta gattgcggcg gagttggaat cctcggtatc gcttgggcct ttggtggtat
     2041 gatcttcatc ctcgtctact gcaccgccgg tatctctggt ggtcacatta acccagcggt
     2101 gacatttggg ctattcttgg cacgtaaagt gtcgttacct agggccctat tgtacataat
     2161 cgctcagtgt ttgggtgcga tttgtggagt tggttttgtc aaagccttcc aaagctctta
     2221 ctacacccgt tacggaggtg gagccaactc tctagccgat ggctacagca cagggaccgg
     2281 tctagccgca gagatcattg gtactttcgt tcttgtctac accgtcttct ctgccactga
     2341 ccccaaacgt agtgccagag actcccacgt tccggtgttg gcgccacttc caatcggatt
     2401 tgccgtgttc atggtacatt tggctaccat tcccattacc ggaaccggaa ttaacccggc
     2461 aaggagtttc ggagctgccg taatctacaa caagagcaag ccatgggatg accactggat
     2521 attttgggtt ggaccattca ttggagctgc gatagctgca ttctaccacc aattcgttct
     2581 aagagcttca ggttctaagt ctcttggatc attcagaagt gctgccaacg tcgtgcccgg
     2641 gggatccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2701 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2761 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2821 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
     2881 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2941 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3001 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3061 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
     3121 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3181 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3241 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3301 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3361 caagtaaagc ggcggcccgg ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag
     3421 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     3481 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     3541 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     3601 taaattatcg cgcgcggtgt catctatgtt actagatccg atgataagct gtcaaacatg
     3661 agagctcccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     3721 aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
     3781 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3841 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3901 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3961 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     4021 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4081 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4141 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4201 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4261 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4321 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4381 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4441 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4501 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4561 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga cacgtgttaa ttcttgaaga
     4621 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4681 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4741 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4801 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4861 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4921 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4981 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5041 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5101 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5161 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5221 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5281 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5341 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5401 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5461 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5521 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5581 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5641 atcgctgaga taggtgcctc actgattaag cattggtaag ttaacagaag accagagggc
     5701 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
     5761 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
     5821 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg
     5881 acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     5941 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     6001 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacct cgaggtcgac
     6061 ggtatcgata agcttatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     6121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     6181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     6241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac
     6301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     6361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     6421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     6481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     6541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     6601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     6661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     6721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     6781 gagctgtaca agcatatgac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
     6841 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     6901 acacaacata tccagtcact atgaatcaac tacttagatg gtattagtga cctgtagtcg
     6961 accgacagcc ttccaaatgt tcttcgggtg atgctgccaa cttagtcgac cgacagcctt
     7021 ccaaatgttc ttctcaaacg gaatcgtcgt atccagccta ctcgctattg tcctcaatgc
     7081 cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt tgtgtgacaa
     7141 aataaaaaca tctacctatt catatacgct agtgtcatag tcctgaaaat catctgcatc
     7201 aagaacaatt tcacaactct tatacttttc tcttacaagt cgttcggctt catctggatt
     7261 ttcagcctct atacttacta aacgtgataa agtttctgta atttctactg tatcgacctg
     7321 cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc aggttaatgg
     7381 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga
     7441 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
     7501 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca
     7561 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
     7621 ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtca
     7681 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc
     7741 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga
     7801 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca
     7861 ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt
     7921 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag
     7981 taagccggat ccacgcggcg tttacgcccc gccctgccac tcatcgcagt actgttgtaa
     8041 ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     8101 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     8161 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     8221 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     8281 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     8341 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     8401 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     8461 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     8521 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     8581 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     8641 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     8701 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     8761 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
     8821 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc
     8881 gtcgggtgat gctgccaact tagtcgacta caggtcacta ataccatcta agtagttgat
     8941 tcatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     9001 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     9061 agtggtctga tctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
     9121 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     9181 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     9241 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgaagcg ct
//
