LOCUS       40924_7641        9007 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-end-n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 9007)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9007)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2488
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      3102..3353
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3354..3520
                     /label=Kan
                     /note="Kan"
                     /regulatory_class="promoter"
     CDS             3521..4312
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4429..4533
                     /label=AmpR promoter
     CDS             4534..5391
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      5400..5765
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5419..5763
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5766..5782
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5791..6507
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    6514..6638
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7070..7372)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7720..8376)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(8377..8479)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8657..8781)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      8846..9001
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
     2221 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaagag atctatggct agctctggaa acaagaacat
     2521 caacgccaaa ttggtattac taggagatgt tggagctgga aaatcaagtc ttgtgctacg
     2581 gtttgtgaaa gatcagtttg ttgaatttca ggaatcaacc attggtgcag cttttttctc
     2641 tcaaacattg gctgtgaatg atgcgactgt gaagtttgag atatgggata cagctggtca
     2701 ggaacgatac cacagtttgg ctccaatgta ctacaggggt gcagctgctg ctattattgt
     2761 ctttgacatt actaatcaag cctcatttga gagggcgaag aaatgggttc aggaactgca
     2821 ggcacaaggt aaccctaata tggtgatggc tcttgctgga aacaaagctg atttattaga
     2881 cgcaaggaag gtgtctgcag aggaggcaga gatatatgct caagagaaca gccttttctt
     2941 tatggaaacc tcagcgaaga ccgcaacaaa tgtcaaagac atattctacg aaatagcgaa
     3001 aaggctacca cgtgtacagc cagctgaaaa cccgacagga atggttctcc caaacgggcc
     3061 aggggctacg gcagtgagtt catcgtgttg tgcttagacc ggtcgttcaa acatttggca
     3121 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3181 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3241 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3301 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atccgatgat
     3361 aagctgtcaa acatgagagc tcccggaatt gccagctggg gcgccctctg gtaaggttgg
     3421 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg
     3481 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     3541 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3601 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3661 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     3721 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3781 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3841 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3901 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3961 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4021 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     4081 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4141 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4201 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4261 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgacacgt
     4321 gttaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4381 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4501 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4561 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4621 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4681 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4741 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4801 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4861 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4921 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4981 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5041 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5101 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5161 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5221 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5281 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5341 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaagttaac
     5401 agaagaccag agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
     5461 attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aagatggctt
     5521 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctaccgacag
     5581 tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag acgttccaac
     5641 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
     5701 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     5761 gacctcgagg tcgacggtat cgataagctt atggtgagca agggcgagga gctgttcacc
     5821 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5881 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5941 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
     6001 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     6061 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     6121 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     6181 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     6241 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     6301 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     6361 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     6421 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     6481 actctcggca tggacgagct gtacaagcat atgacaagtt tgtacaaaaa agctgaacga
     6541 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     6601 acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt agatggtatt
     6661 agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct gccaacttag
     6721 tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca gcctactcgc
     6781 tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc
     6841 ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt catagtcctg
     6901 aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc
     6961 ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc
     7021 tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
     7081 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
     7141 ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
     7201 ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac
     7261 tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca
     7321 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
     7381 cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc
     7441 ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg
     7501 gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt
     7561 cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg
     7621 ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg
     7681 ttatctggct tttagtaagc cggatccacg cggcgtttac gccccgccct gccactcatc
     7741 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg
     7801 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     7861 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     7921 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     7981 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     8041 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     8101 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     8161 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     8221 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     8281 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     8341 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     8401 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     8461 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     8521 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     8581 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gactacaggt cactaatacc
     8641 atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     8701 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     8761 agctttcttg tacaaagtgg tctgatctag agtccgcaaa aatcaccagt ctctctctac
     8821 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     8881 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     8941 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     9001 aagcgct
//
