LOCUS       40924_7631        8416 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-cyt-n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2488
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2510..2762
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2763..2929
                     /label=Kan
                     /note="Kan"
                     /regulatory_class="promoter"
     CDS             2930..3721
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3838..3942
                     /label=AmpR promoter
     CDS             3943..4800
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4809..5174
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        4828..5172
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5175..5191
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5200..5916
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    5923..6047
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6479..6781)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7129..7785)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7786..7888)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8066..8190)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      8255..8410
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
     2221 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaagta aagcggcggc ccggctgcag atcgttcaaa
     2521 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2581 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2641 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     2701 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     2761 tccgatgata agctgtcaaa catgagagct cccggaattg ccagctgggg cgccctctgg
     2821 taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg
     2881 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     2941 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3001 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3061 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     3121 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3181 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3241 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3301 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     3361 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     3421 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     3481 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     3541 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     3601 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     3661 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     3721 ctgacacgtg ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3781 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3961 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4021 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4081 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4141 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4201 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4261 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4321 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4381 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4441 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4501 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4561 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4621 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4681 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4741 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4801 taagttaaca gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa
     4861 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     4921 agatggcttc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
     4981 taccgacagt ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga
     5041 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     5101 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     5161 tttggagagg acctcgaggt cgacggtatc gataagctta tggtgagcaa gggcgaggag
     5221 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5281 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     5341 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     5401 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5461 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5521 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5581 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     5641 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     5701 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     5761 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     5821 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     5881 gccgggatca ctctcggcat ggacgagctg tacaagcata tgacaagttt gtacaaaaaa
     5941 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     6001 aaacagacta cataatactg taaaacacaa catatccagt cactatgaat caactactta
     6061 gatggtatta gtgacctgta gtcgaccgac agccttccaa atgttcttcg ggtgatgctg
     6121 ccaacttagt cgaccgacag ccttccaaat gttcttctca aacggaatcg tcgtatccag
     6181 cctactcgct attgtcctca atgccgtatt aaatcataaa aagaaataag aaaaagaggt
     6241 gcgagcctct tttttgtgtg acaaaataaa aacatctacc tattcatata cgctagtgtc
     6301 atagtcctga aaatcatctg catcaagaac aatttcacaa ctcttatact tttctcttac
     6361 aagtcgttcg gcttcatctg gattttcagc ctctatactt actaaacgtg ataaagtttc
     6421 tgtaatttct actgtatcga cctgcagact ggctgtgtat aagggagcct gacatttata
     6481 ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag
     6541 ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc
     6601 agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca
     6661 gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct
     6721 gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca
     6781 tttcaccagc ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc
     6841 tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca
     6901 ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact
     6961 gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga
     7021 catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata
     7081 cgcatactgt tatctggctt ttagtaagcc ggatccacgc ggcgtttacg ccccgccctg
     7141 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     7201 gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     7261 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     7321 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     7381 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     7441 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     7501 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     7561 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     7621 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     7681 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     7741 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     7801 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     7861 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     7921 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     7981 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg actacaggtc
     8041 actaatacca tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta
     8101 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     8161 ttctcgttca gctttcttgt acaaagtggt ctgatctaga gtccgcaaaa atcaccagtc
     8221 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
     8281 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     8341 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     8401 aatccagtga agcgct
//
