LOCUS       40924_7626        8444 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-cyt-c, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2488
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2510..2762
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2763..2929
                     /label=Kan
                     /note="Kan"
                     /regulatory_class="promoter"
     CDS             2930..3721
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3838..3942
                     /label=AmpR promoter
     CDS             3943..4800
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4809..5174
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        4828..5172
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5175..5191
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5225..5349
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5781..6083)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(6431..7087)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7088..7190)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(7368..7492)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7503..8219
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      8283..8438
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
     2221 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaagta aagcggcggc ccggctgcag atcgttcaaa
     2521 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2581 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2641 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     2701 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     2761 tccgatgata agctgtcaaa catgagagct cccggaattg ccagctgggg cgccctctgg
     2821 taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg
     2881 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     2941 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3001 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3061 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     3121 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3181 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3241 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3301 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     3361 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     3421 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     3481 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     3541 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     3601 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     3661 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     3721 ctgacacgtg ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3781 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3961 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4021 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4081 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4141 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4201 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4261 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4321 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4381 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4441 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4501 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4561 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4621 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4681 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4741 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4801 taagttaaca gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa
     4861 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     4921 agatggcttc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
     4981 taccgacagt ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga
     5041 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     5101 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     5161 tttggagagg acctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc
     5221 atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     5281 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     5341 gtcactatga atcaactact tagatggtat tagtgacctg tagtcgaccg acagccttcc
     5401 aaatgttctt cgggtgatgc tgccaactta gtcgaccgac agccttccaa atgttcttct
     5461 caaacggaat cgtcgtatcc agcctactcg ctattgtcct caatgccgta ttaaatcata
     5521 aaaagaaata agaaaaagag gtgcgagcct cttttttgtg tgacaaaata aaaacatcta
     5581 cctattcata tacgctagtg tcatagtcct gaaaatcatc tgcatcaaga acaatttcac
     5641 aactcttata cttttctctt acaagtcgtt cggcttcatc tggattttca gcctctatac
     5701 ttactaaacg tgataaagtt tctgtaattt ctactgtatc gacctgcaga ctggctgtgt
     5761 ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca
     5821 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc
     5881 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
     5941 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
     6001 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
     6061 taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt
     6121 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
     6181 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
     6241 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
     6301 ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat attcttatac
     6361 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac
     6421 gcggcgttta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     6481 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     6541 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     6601 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     6661 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     6721 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     6781 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     6841 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     6901 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     6961 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     7021 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     7081 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     7141 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     7201 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     7261 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     7321 ccaacttagt cgactacagg tcactaatac catctaagta gttgattcat agtgactgga
     7381 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     7441 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgccacgg
     7501 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     7561 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     7621 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     7681 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     7741 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7801 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7861 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7921 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     7981 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     8041 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     8101 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     8161 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     8221 aatctagagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc
     8281 tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc
     8341 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     8401 caataaaatt tctaattcct aaaaccaaaa tccagtgaag cgct
//
