LOCUS       40924_7621        8593 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-cg-n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8593)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8593)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1949..2665
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2687..2939
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2940..3106
                     /label=Kan
                     /note="Kan"
                     /regulatory_class="promoter"
     CDS             3107..3898
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4015..4119
                     /label=AmpR promoter
     CDS             4120..4977
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4986..5351
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5005..5349
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5352..5368
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5377..6093
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    6100..6224
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6656..6958)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7306..7962)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7963..8065)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8243..8367)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      8432..8587
                     /note="35Ster; CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggctagc gggagcagat cagtgggtag
     1801 cagcagcagc aaatggaggt actgcaaccc tactagttac ttgaagcgcc caaagcgtct
     1861 tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc gtcaaactga
     1921 cgtctcgagg accggtcccg ggggatccat ggtgagcaag ggcgaggagc tgttcaccgg
     1981 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2041 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2101 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     2161 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     2221 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2281 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     2341 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt
     2401 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     2461 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     2521 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     2581 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2641 tctcggcatg gacgagctgt acaagtaaag cggcggcccg gctgcagatc gttcaaacat
     2701 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     2761 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2821 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2881 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcc
     2941 gatgataagc tgtcaaacat gagagctccc ggaattgcca gctggggcgc cctctggtaa
     3001 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg
     3061 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3181 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     3301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     3601 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     3661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     3721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     3781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     3841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     3901 acacgtgtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3961 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4021 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4081 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4141 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4201 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4261 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4321 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4381 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4441 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4501 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4561 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4621 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4681 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4741 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4801 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4861 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4921 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4981 gttaacagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     5041 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaaga
     5101 tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac
     5161 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt
     5221 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     5281 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     5341 ggagaggacc tcgaggtcga cggtatcgat aagcttatgg tgagcaaggg cgaggagctg
     5401 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5461 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5521 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     5581 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5641 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5701 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5761 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5821 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5881 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5941 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     6001 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6061 gggatcactc tcggcatgga cgagctgtac aagcatatga caagtttgta caaaaaagct
     6121 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     6181 cagactacat aatactgtaa aacacaacat atccagtcac tatgaatcaa ctacttagat
     6241 ggtattagtg acctgtagtc gaccgacagc cttccaaatg ttcttcgggt gatgctgcca
     6301 acttagtcga ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tatccagcct
     6361 actcgctatt gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgcg
     6421 agcctctttt ttgtgtgaca aaataaaaac atctacctat tcatatacgc tagtgtcata
     6481 gtcctgaaaa tcatctgcat caagaacaat ttcacaactc ttatactttt ctcttacaag
     6541 tcgttcggct tcatctggat tttcagcctc tatacttact aaacgtgata aagtttctgt
     6601 aatttctact gtatcgacct gcagactggc tgtgtataag ggagcctgac atttatattc
     6661 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     6721 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
     6781 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
     6841 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
     6901 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
     6961 caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca
     7021 gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc
     7081 tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat
     7141 agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat
     7201 acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc
     7261 atactgttat ctggctttta gtaagccgga tccacgcggc gtttacgccc cgccctgcca
     7321 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     7381 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     7441 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     7501 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     7561 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     7621 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     7681 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     7741 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     7801 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     7861 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     7921 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     7981 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     8041 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt
     8101 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     8161 tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttagtcgact acaggtcact
     8221 aataccatct aagtagttga ttcatagtga ctggatatgt tgtgttttac agtattatgt
     8281 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     8341 tcgttcagct ttcttgtaca aagtggtctg atctagagtc cgcaaaaatc accagtctct
     8401 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     8461 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     8521 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     8581 ccagtgaagc gct
//
