LOCUS       40924_7616        8621 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-cg-c, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8621)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8621)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1949..2665
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2687..2939
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2940..3106
                     /label=Kan
                     /note="Kan"
                     /regulatory_class="promoter"
     CDS             3107..3898
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4015..4119
                     /label=AmpR promoter
     CDS             4120..4977
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4986..5351
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5005..5349
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5352..5368
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5402..5526
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5958..6260)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(6608..7264)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7265..7367)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(7545..7669)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7680..8396
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      8460..8615
                     /note="35Ster; CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatggctagc gggagcagat cagtgggtag
     1801 cagcagcagc aaatggaggt actgcaaccc tactagttac ttgaagcgcc caaagcgtct
     1861 tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc gtcaaactga
     1921 cgtctcgagg accggtcccg ggggatccat ggtgagcaag ggcgaggagc tgttcaccgg
     1981 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2041 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2101 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     2161 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     2221 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2281 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     2341 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt
     2401 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     2461 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     2521 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     2581 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2641 tctcggcatg gacgagctgt acaagtaaag cggcggcccg gctgcagatc gttcaaacat
     2701 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     2761 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2821 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2881 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcc
     2941 gatgataagc tgtcaaacat gagagctccc ggaattgcca gctggggcgc cctctggtaa
     3001 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg
     3061 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3181 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     3301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     3601 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     3661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     3721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     3781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     3841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     3901 acacgtgtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3961 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4021 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4081 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4141 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4201 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4261 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4321 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4381 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4441 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4501 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4561 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4621 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4681 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4741 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4801 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4861 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4921 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4981 gttaacagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     5041 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaaga
     5101 tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac
     5161 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt
     5221 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     5281 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     5341 ggagaggacc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccatc
     5401 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     5461 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     5521 actatgaatc aactacttag atggtattag tgacctgtag tcgaccgaca gccttccaaa
     5581 tgttcttcgg gtgatgctgc caacttagtc gaccgacagc cttccaaatg ttcttctcaa
     5641 acggaatcgt cgtatccagc ctactcgcta ttgtcctcaa tgccgtatta aatcataaaa
     5701 agaaataaga aaaagaggtg cgagcctctt ttttgtgtga caaaataaaa acatctacct
     5761 attcatatac gctagtgtca tagtcctgaa aatcatctgc atcaagaaca atttcacaac
     5821 tcttatactt ttctcttaca agtcgttcgg cttcatctgg attttcagcc tctatactta
     5881 ctaaacgtga taaagtttct gtaatttcta ctgtatcgac ctgcagactg gctgtgtata
     5941 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     6001 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
     6061 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
     6121 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
     6181 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
     6241 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat
     6301 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
     6361 acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc
     6421 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
     6481 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
     6541 aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccacgcg
     6601 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     6661 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     6721 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     6781 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     6841 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     6901 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     6961 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     7021 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     7081 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     7141 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     7201 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     7261 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     7321 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     7381 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     7441 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     7501 acttagtcga ctacaggtca ctaataccat ctaagtagtt gattcatagt gactggatat
     7561 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     7621 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt gccacggcca
     7681 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     7741 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     7801 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     7861 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     7921 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     7981 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     8041 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     8101 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     8161 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     8221 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     8281 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     8341 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     8401 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     8461 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     8521 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     8581 taaaatttct aattcctaaa accaaaatcc agtgaagcgc t
//
