LOCUS       62056_4245        5303 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             676..1392
                     /codon_start=1
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     CDS             1427..1456
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1472..1489
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    complement(1508..1641)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1774..2145)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2164..2211)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(2259..2575)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2597..3759
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3835..3876
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             3886..3903
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    3932..4156
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4288..4876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg ccaccatggt gagcaagcag atcctgaaga acaccggcct gcaggagatc
      721 atgagcttca aggtgaacct ggagggcgtg gtgaacaacc acgtgttcac catggagggc
      781 tgcggcaagg gcaacatcct gttcggcaac cagctggtgc agatccgcgt gaccaagggc
      841 gcccccctgc ccttcgcctt cgacatcctg agccccgcct tccagtacgg caaccgcacc
      901 ttcaccaagt accccgagga catcagcgac ttcttcatcc agagcttccc cgccggcttc
      961 gtgtacgagc gcaccctgcg ctacgaggac ggcggcctgg tggagatccg cagcgacatc
     1021 aacctgatcg aggagatgtt cgtgtaccgc gtggagtaca agggccgcaa cttccccaac
     1081 gacggccccg tgatgaagaa gaccatcacc ggcctgcagc ccagcttcga ggtggtgtac
     1141 atgaacgacg gcgtgctggt gggccaggtg atcctggtgt accgcctgaa cagcggcaag
     1201 ttctacagct gccacatgcg caccctgatg aagagcaagg gcgtggtgaa ggacttcccc
     1261 gagtaccact tcatccagca ccgcctggag aagacctacg tggaggacgg cggcttcgtg
     1321 gagcagcacg agaccgccat cgcccagctg accagcctgg gcaagcccct gggcagcctg
     1381 cacgagtggg tgtaaatcga tcttaagcag tacttctaga ggatccgaac aaaaactcat
     1441 ctcagaagag gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttgatc
     1501 cccgggaatt cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1561 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1621 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1681 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1741 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1801 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1861 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1921 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1981 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     2041 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     2101 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     2161 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     2221 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     2281 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     2341 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     2401 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     2461 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2521 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctcgt
     2581 cgagctagct tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     2641 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     2701 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     2761 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     2821 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     2881 ttgcgtgcct tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga
     2941 gccaggggcg ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3001 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3061 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3121 caagatagtc ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttgggccc
     3181 gcggccggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3241 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3301 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3361 gttgcgtgag cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg
     3421 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3481 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3541 tagttctgga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3601 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3661 ttctcgttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3721 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa cacgtggtcg cggccgcttc
     3781 gaaggtacca gcacagtgga ctcgagagat ctggccggct gggcccgttt cgaaggtaag
     3841 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     3901 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3961 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4021 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4081 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4141 gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac ggttatccac
     4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4921 cgaaaactca cgttaaggga ttttggtcat gacattaacc tataaaaata ggcgtatcac
     4981 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5041 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5101 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     5161 tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5221 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     5281 cgggcctctt cgctattacg cca
//
