LOCUS       62056_4240        5293 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             719..748
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             764..781
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    complement(800..933)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1066..1437)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1456..1503)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(1551..1867)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1889..3051
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3071..3787
                     /codon_start=1
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     CDS             3825..3866
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             3876..3893
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    3922..4146
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4278..4866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg cattcctgca ggtcgacatc gatcttaagc agtacttcta gaggatccga
      721 acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcacca
      781 ttgagtttga tccccgggaa ttcagacatg ataagataca ttgatgagtt tggacaaacc
      841 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      901 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
      961 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     1021 ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc
     1081 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     1141 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     1201 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     1261 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     1321 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     1381 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt
     1441 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     1501 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     1561 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     1621 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     1681 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     1741 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     1801 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     1861 ccacaccctc gtcgagctag cttcgtgagg ctccggtgcc cgtcagtggg cagagcgcac
     1921 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     1981 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2041 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2101 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     2161 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctccagtacg tgattcttga
     2221 tcccgagctg gagccagggg cgggccttgc gctttaggag ccccttcgcc tcgtgcttga
     2281 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     2341 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     2401 ctttttttct ggcaagatag tcttgtaaat gcgggccagg atctgcacac tggtatttcg
     2461 gtttttgggc ccgcggccgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     2521 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     2581 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     2641 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tccagggggc
     2701 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     2761 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     2821 aggcacctcg attagttctg gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     2881 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     2941 cacttgatgt aattctcgtt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     3001 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aacacgtggt
     3061 cgcggccacc atggtgagca agcagatcct gaagaacacc ggcctgcagg agatcatgag
     3121 cttcaaggtg aacctggagg gcgtggtgaa caaccacgtg ttcaccatgg agggctgcgg
     3181 caagggcaac atcctgttcg gcaaccagct ggtgcagatc cgcgtgacca agggcgcccc
     3241 cctgcccttc gccttcgaca tcctgagccc cgccttccag tacggcaacc gcaccttcac
     3301 caagtacccc gaggacatca gcgacttctt catccagagc ttccccgccg gcttcgtgta
     3361 cgagcgcacc ctgcgctacg aggacggcgg cctggtggag atccgcagcg acatcaacct
     3421 gatcgaggag atgttcgtgt accgcgtgga gtacaagggc cgcaacttcc ccaacgacgg
     3481 ccccgtgatg aagaagacca tcaccggcct gcagcccagc ttcgaggtgg tgtacatgaa
     3541 cgacggcgtg ctggtgggcc aggtgatcct ggtgtaccgc ctgaacagcg gcaagttcta
     3601 cagctgccac atgcgcaccc tgatgaagag caagggcgtg gtgaaggact tccccgagta
     3661 ccacttcatc cagcaccgcc tggagaagac ctacgtggag gacggcggct tcgtggagca
     3721 gcacgagacc gccatcgccc agctgaccag cctgggcaag cccctgggca gcctgcacga
     3781 gtgggtgtaa ctcgagagat ctggccggct gggcccgttt cgaaggtaag cctatcccta
     3841 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
     3901 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3961 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4021 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4081 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4141 ctatggcttc tgaggcggaa agaaccagtg gcggtaatac ggttatccac agaatcaggg
     4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4681 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4921 cgttaaggga ttttggtcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4981 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5101 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5161 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     5221 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     5281 cgctattacg cca
//
