LOCUS       pBridge.        6527 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast three-hybrid vector for expressing a """"bait"""" protein 
            fused to the GAL4 DNA-binding domain, plus an additional bridge 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    pBridge
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..905
                     /label=MCS I
                     /note="MCS 1"
                     /note="multiple cloning site"
     terminator      921..1108
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1164..1835)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(1836..1937)
                     /label=TRP1 promoter
     primer_bind     complement(1951..1967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1975..1991)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1999..2029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2044..2065)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2259..2609
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             2619..2621
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2631..2657
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2679..2699
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    2700..2723
                     /label=MCS II
                     /note="MCS 2"
                     /note="multiple cloning site"
     terminator      2825..3095
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     rep_origin      complement(3357..3945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4119..4976)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4977..5081)
                     /label=AmpR promoter
     rep_origin      5363..6527
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac
      121 agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact
      181 ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt
      241 ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccgtcgacc
      901 tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag
      961 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     1021 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     1081 tcgctcttat tgaccacacc tctaccggca tgccggcaag tgcacaaaca atacttaaat
     1141 aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
     1201 gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
     1261 aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
     1321 ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca
     1381 cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct
     1441 gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg
     1501 aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg
     1561 taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag
     1621 tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca
     1681 ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca
     1741 tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat
     1801 ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta
     1861 atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt
     1921 cttttttcga ccgaattaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     1981 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2041 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2101 aaacctgtcg tgccaggaag atccgaggcc tagcttctaa ttcttccaac atacaatggg
     2161 agtttggccg agtggtttaa ggcgtcagat ttaggtggat ttaacctcta aaatctctga
     2221 tatcttcgga tgcaagggtt cgaatccctt agctctcatt attttttgct ttttctcttg
     2281 aggtcacatg atcgcaaaat ggcaaatggc acgtgaagct gtcgatattg gggaactgtg
     2341 gtggttggca aatgactaat taagttagtc aaggcgccat cctcatgaaa actgtgtaac
     2401 ataataaccg aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa
     2461 aataagatat atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct
     2521 tgctctcttg tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata
     2581 gatacaattc tattaccccc atccatacaa tgggccatat ggcttctagc tatccttatg
     2641 acgtgcctga ctatgccagc ctgggaggac cttctagtcc taagaagaag agaaaggtgg
     2701 cggccgcatt agcccgaaga tcttcgggct gatctcccat gtctctactg gtggtggtgc
     2761 ttctttggaa ttattggaag gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa
     2821 ataaattgaa ttgaattgaa atcgatagat caattttttt cttttctctt tccccatcct
     2881 ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata tacgtatata
     2941 tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa attcgctcct
     3001 cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgtt cgggttcagc
     3061 gtattttaag tttaataact cgaaaattct gcgttcgtta aagctaggcc tcggatcttc
     3121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3781 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5101 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5161 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5221 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5281 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5341 attgtactga gagtgcacca taacgcattt aagcataaac acgcactatg ccgttcttct
     5401 catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta
     5461 tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc
     5521 tattccgaag ttcctattct ctagctagaa agtataggaa cttcagagcg cttttgaaaa
     5581 ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc
     5641 tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata
     5701 tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca
     5761 tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa
     5821 attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc
     5881 tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag
     5941 aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat
     6001 ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa
     6061 acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt
     6121 cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc
     6181 acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc
     6241 atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt
     6301 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     6361 gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa tagcgctttc
     6421 gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct
     6481 ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgtt
//
