LOCUS       V009096                 9085 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009096
VERSION     V009096
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Thole V, Worland B, Vain P.
  TITLE     pBrachyTAG vector system: New tools for DNA tagging and trapping in
            grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2010) Crop Genetics, John Innes Centre, Norwich
            Research Park, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
REFERENCE   3  (bases 1 to 9085)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-2010) Crop Genetics, John Innes Centre, Norwich Research
            Park, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border sequence No.2
                     belonging to a double left border sequence separated by a
                     spacer sequence from left border No.1"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic unique sequence containing
                     forward and reverse primer sequences"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4672..4964)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     regulatory      complement(4974..4985)
                     /gene="sgfp(S65T)-ER"
                     /note="C-terminal HDEL signal sequence for protein
                     secretion and retention in the ER"
                     /regulatory_class="other"
     CDS             complement(4986..5699)
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     regulatory      complement(5700..5765)
                     /gene="sgfp(S65T)-ER"
                     /note="N-terminal ER signal peptide sequence from
                     Arabidopsis thaliana basic chitinase"
                     /regulatory_class="other"
     promoter        complement(5774..7770)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    7820..7923
                     /note="UniBox2; synthetic unique sequence containing
                     forward and reverse primer sequences"
     misc_feature    7930..7954
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7955..7978
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             8182..8982
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccgctac gttagatcgt attctacagc ttccgtatca gccttgaata
     2641 gctctgcgtc cagtatctcg tgcagctaaa ggctacctca gtaagcgagt aacgcggccg
     2701 cttattaatt aagatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     2761 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     2821 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     2881 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     2941 tttattgcca aatgtttgaa cgatcgggga aattcgagct ctccggatcc cggtcggcat
     3001 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     3061 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     3121 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     3181 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     3241 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     3301 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     3361 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     3421 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     3481 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     3541 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     3601 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     3661 gcagcgatcg catccatggc ctccgcgacc ggctgcagtt atcatcatca tcatagacac
     3721 acgaaataaa gtaatcagat tatcagttaa agctatgtaa tatttacacc ataaccaatc
     3781 aattaaaaaa tagatcagtt taaagaaaga tcaaagctca aaaaaataaa aagagaaaag
     3841 ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt acctgcagaa cagcgggcag
     3901 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     3961 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     4021 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     4081 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     4141 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     4201 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
     4261 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     4321 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     4381 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     4441 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     4501 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     4561 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     4621 tagacgagag tgtcgtgctc caccatgttg acttaattaa aaggccgtac ggttaaccat
     4681 catttagtga cacaattaac ttaaatttag aagagactaa tttcaaccca caagatggaa
     4741 atgcaaggac aatgggttct tgaagttatt tggaaacaaa gcaacacaac acaatagctt
     4801 attcattagt tctcatccat taatttccat tgaagtccga aaacagaaac caaaaataca
     4861 ggatgatgaa atctcataaa gcaaaaacaa atgggaatgt agtttaagta caacaaacaa
     4921 agtggggtgg ctacagatgc tttgcaaatt tttcaatgac ttacggtacc ttaaagctca
     4981 tcatgcttgt acagctcgtc catgccgtga gtgatcccgg cggcggtcac gaactccagc
     5041 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     5101 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     5161 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     5221 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     5281 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     5341 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     5401 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     5461 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtgaa ggtggtcacg
     5521 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     5581 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     5641 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacg
     5701 aattcggccg aggataatga taggagaagt gaaaagatga gaaagagaaa aagattagtc
     5761 ttcatggaag cttctgcaga agtaacacca aacaacaggg tgagcatcga caaaagaaac
     5821 agtaccaagc aaataaatag cgtatgaagg cagggctaaa aaaatccaca tatagctgct
     5881 gcatatgcca tcatccaagt atatcaagat caaaataatt ataaaacata cttgtttatt
     5941 ataatagata ggtactcaag gttagagcat atgaatagat gctgcatatg ccatcatgta
     6001 tatgcatcag taaaacccac atcaacatgt atacctatcc tagatcgata tttccatcca
     6061 tcttaaactc gtaactatga agatgtatga cacacacata cagttccaaa attaataaat
     6121 acaccaggta gtttgaaaca gtattctact ccgatctaga acgaatgaac gaccgcccaa
     6181 ccacaccaca tcatcacaac caagcgaaca aaaagcatct ctgtatatgc atcagtaaaa
     6241 cccgcatcaa catgtatacc tatcctagat cgatatttcc atccatcatc ttcaattcgt
     6301 aactatgaat atgtatggca cacacataca gatccaaaat taataaatcc accaggtagt
     6361 ttgaaacaga attaattcta ctccgatcta gaacgaccgc ccaaccagac cacatcatca
     6421 caaccaagac aaaaaaaagc atgaaaagat gacccgacaa acaagtgcac ggcatatatt
     6481 gaaataaagg aaaagggcaa accaaaccct atgcaacgaa acaaaaaaaa tcatgaaatc
     6541 gatcccgtct gcggaacggc tagagccatc ccaggattcc ccaaagagaa acactggcaa
     6601 gttagcaatc agaacgtgtc tgacgtacag gtcgcatccg tgtacgaacg ctagcagcac
     6661 ggatctaaca caaacacgga tctaacacaa acatgaacag aagtagaact accgggccct
     6721 aaccatggac cggaacgccg atctagagaa ggtagagagg gggggggggg gaggacgagc
     6781 ggcgtacctt gaagcggagg tgccgacggg tggatttggg ggagatctgg ttgtgtgtgt
     6841 gtgcgctccg aacaacacga ggttggggaa agagggtgtg gagggggtgt ctatttatta
     6901 cggcgggcga ggaagggaaa gcgaaggagc ggtgggaaag gaatcccccg tagctgccgg
     6961 tgccgtgaga ggaggaggag gccgcctgcc gtgccggctc acgtctgccg ctccgccacg
     7021 caatttctgg atgccgacag cggagcaagt ccaacggtgg agcggaactc tcgagagggg
     7081 tccagaggca gcgacagaga tgccgtgccg tctgcttcgc ttggcccgac gcgacgctgc
     7141 tggttcgctg gttggtgtcc gttagactcg tcgacggcgt ttaacaggct ggcattatct
     7201 actcgaaaca agaaaaatgt ttccttagtt tttttaattt cttaaagggt atttgtttaa
     7261 tttttagtca ctttatttta ttctatttta tatctaaatt attaaataaa aaaactaaaa
     7321 tagagtttta gttttcttaa tttagaggct aaaatagaat aaaatagatg tactaaaaaa
     7381 attagtctat aaaaaccatt aaccctaaac cctaaatgga tgtactaata aaatggatga
     7441 agtattatat aggtgaagct atttgcaaaa aaaaaggaga acacatgcac actaaaaaga
     7501 taaaactgta gagtcctgtt gtcaaaatac tcaattgtcc tttagaccat gtctaactgt
     7561 tcatttatat gattctctaa aacactgata ttattgtagt actatagatt atattattcg
     7621 tagagtaaag tttaaatata tgtataaaga tagataaact gcacttcaaa caagtgtgac
     7681 aaaaaaaata tgtggtaatt ttttataact tagacatgca atgctcatta tctctagaga
     7741 ggggcacgac cgggtcacgc tgcactgcag gaattcaagc tggcatcgag attcaaacta
     7801 gtctcgagtc gacaggcctt tctaacctgt cgaggatacg tgataagcgg gagcttagcg
     7861 gagctcgttg caatcaaagc tcgatacgtc gagacttaga ggatctacga cagtccacag
     7921 aacaggcctt gacaggatat attggcgggt aaactaagtc gctgtatgtg tttgtttgtt
     7981 taaacccggg aggctcgcgg cggacgcacg acgccggggc gagaccatag gcgatctcct
     8041 aaatcaatag tagctgtaac ctcgaagcgt ttcacttgta acaacgattg agaatttttg
     8101 tcataaaatt gaaatacttg gttcgcattt ttgtcatccg cggtcagccg caattctgac
     8161 gaactgccca tttagctgga gatgattgta catccttcac gtgaaaattt ctcaagcgct
     8221 gtgaacaagg gttcagattt tagattgaaa ggtgagccgt tgaaacacgt tcttcttgtc
     8281 gatgacgacg tcgctatgcg gcatcttatt attgaatacc ttacgatcca cgccttcaaa
     8341 gtgaccgcgg tagccgacag cacccagttc acaagagtac tctcttccgc gacggtcgat
     8401 gtcgtggttg ttgatctaaa tttaggtcgt gaagatgggc tcgagatcgt tcgtaatctg
     8461 gcggcaaagt ctgatattcc aatcataatt atcagtggcg accgccttga ggagacggat
     8521 aaagttgttg cactcgagct aggagcaagt gattttatcg ctaagccgtt cagtatcaga
     8581 gagtttctag cacgcattcg ggttgccttg cgcgtgcgcc ccaacgttgt ccgctccaaa
     8641 gaccgacggt ctttttgttt tactgactgg acacttaatc tcaggcaacg tcgcttgatg
     8701 tccgaagctg gcggtgaggt gaaacttacg gcaggtgagt tcaatcttct cctcgcgttt
     8761 ttagagaaac cccgcgacgt tctatcgcgc gagcaacttc tcattgccag tcgagtacgc
     8821 gacgaggagg tttatgacag gagtatagat gttctcattt tgaggctgcg ccgcaaactt
     8881 gaggcggatc cgtcaagccc tcaactgata aaaacagcaa gaggtgccgg ttatttcttt
     8941 gacgcggacg tgcaggtttc gcacgggggg acgatggcag cctgagccaa ttgcatttgc
     9001 ctcttaatta tctggctcaa agggtgactg aggagtaagc gatgtgccca tcacactgcc
     9061 cgggcatgca agctgatctg gatct
//
