LOCUS       V009099                 6873 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009099
VERSION     V009099
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Thole V, Worland B, Vain P.
  TITLE     pBrachyTAG vector system: New tools for DNA tagging and trapping in
            grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2010) Crop Genetics, John Innes Centre, Norwich
            Research Park, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
REFERENCE   3  (bases 1 to 6873)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6873)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2010) Crop Genetics, John Innes Centre, Norwich Research
            Park, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA border sequence No.2 belonging
                     to a double left border sequence separated by a spacer
                     sequence from left border No.1"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic unique sequence containing
                     forward and reverse primer sequences"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4672..4964)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     CDS             complement(4982..5698)
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    5718..5742
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5743..5766
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             5970..6770
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccgctac gttagatcgt attctacagc ttccgtatca gccttgaata
     2641 gctctgcgtc cagtatctcg tgcagctaaa ggctacctca gtaagcgagt aacgcggccg
     2701 cttattaatt aagatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     2761 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     2821 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     2881 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     2941 tttattgcca aatgtttgaa cgatcgggga aattcgagct ctccggatcc cggtcggcat
     3001 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     3061 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     3121 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     3181 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     3241 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     3301 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     3361 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     3421 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     3481 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     3541 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     3601 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     3661 gcagcgatcg catccatggc ctccgcgacc ggctgcagtt atcatcatca tcatagacac
     3721 acgaaataaa gtaatcagat tatcagttaa agctatgtaa tatttacacc ataaccaatc
     3781 aattaaaaaa tagatcagtt taaagaaaga tcaaagctca aaaaaataaa aagagaaaag
     3841 ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt acctgcagaa cagcgggcag
     3901 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     3961 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     4021 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     4081 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     4141 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     4201 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
     4261 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     4321 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     4381 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     4441 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     4501 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     4561 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     4621 tagacgagag tgtcgtgctc caccatgttg acttaattaa aaggccgtac ggttaaccat
     4681 catttagtga cacaattaac ttaaatttag aagagactaa tttcaaccca caagatggaa
     4741 atgcaaggac aatgggttct tgaagttatt tggaaacaaa gcaacacaac acaatagctt
     4801 attcattagt tctcatccat taatttccat tgaagtccga aaacagaaac caaaaataca
     4861 ggatgatgaa atctcataaa gcaaaaacaa atgggaatgt agtttaagta caacaaacaa
     4921 agtggggtgg ctacagatgc tttgcaaatt tttcaatgac ttacggtacc cggccgcttt
     4981 acttgtacag ctcgtccatg ccgtgagtga tcccggcggc ggtcacgaac tccagcagga
     5041 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     5101 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     5161 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     5221 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     5281 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     5341 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     5401 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     5461 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtgaaggtg gtcacgaggg
     5521 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     5581 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     5641 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     5701 ctcgagtcga caggccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt
     5761 tgtttgttta aacccgggag gctcgcggcg gacgcacgac gccggggcga gaccataggc
     5821 gatctcctaa atcaatagta gctgtaacct cgaagcgttt cacttgtaac aacgattgag
     5881 aatttttgtc ataaaattga aatacttggt tcgcattttt gtcatccgcg gtcagccgca
     5941 attctgacga actgcccatt tagctggaga tgattgtaca tccttcacgt gaaaatttct
     6001 caagcgctgt gaacaagggt tcagatttta gattgaaagg tgagccgttg aaacacgttc
     6061 ttcttgtcga tgacgacgtc gctatgcggc atcttattat tgaatacctt acgatccacg
     6121 ccttcaaagt gaccgcggta gccgacagca cccagttcac aagagtactc tcttccgcga
     6181 cggtcgatgt cgtggttgtt gatctaaatt taggtcgtga agatgggctc gagatcgttc
     6241 gtaatctggc ggcaaagtct gatattccaa tcataattat cagtggcgac cgccttgagg
     6301 agacggataa agttgttgca ctcgagctag gagcaagtga ttttatcgct aagccgttca
     6361 gtatcagaga gtttctagca cgcattcggg ttgccttgcg cgtgcgcccc aacgttgtcc
     6421 gctccaaaga ccgacggtct ttttgtttta ctgactggac acttaatctc aggcaacgtc
     6481 gcttgatgtc cgaagctggc ggtgaggtga aacttacggc aggtgagttc aatcttctcc
     6541 tcgcgttttt agagaaaccc cgcgacgttc tatcgcgcga gcaacttctc attgccagtc
     6601 gagtacgcga cgaggaggtt tatgacagga gtatagatgt tctcattttg aggctgcgcc
     6661 gcaaacttga ggcggatccg tcaagccctc aactgataaa aacagcaaga ggtgccggtt
     6721 atttctttga cgcggacgtg caggtttcgc acggggggac gatggcagcc tgagccaatt
     6781 gcatttgcct cttaattatc tggctcaaag ggtgactgag gagtaagcga tgtgcccatc
     6841 acactgcccg ggcatgcaag ctgatctgga tct
//
