LOCUS       V009100                 9009 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009100
VERSION     V009100
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Thole V, Worland B, Schieszl K, Wright JM, Vain P.
  TITLE     pBrachyTAG vector system: New tools for DNA tagging and trapping in
            grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2010) Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 9009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2010) Crop Genetics, John Innes Centre, Colney Lane, Norwich
            NR4 7UH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="26-nt long spacer sequence separating left border
                     No.1 and No.2 of the double left border sequence (Thole et
                     al., 2007)"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border sequence No.2
                     belonging to a double left border sequence separated by a
                     26-nt long spacer sequence from left border No.1 (Thole et
                     al., 2007)"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic 96-nt long unique sequence
                     containing forward and reverse primer sequences;
                     '>http://www.brachytag.org/vector-system.htm"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4678..4930)
                     /note="NOS-ter; poly(A) signal of nopaline synthase gene"
                     /regulatory_class="terminator"
     terminator      4678..4930
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4964..5680)
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        complement(5699..7695)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    7744..7847
                     /note="UniBox2; synthetic 104-nt long unique sequence
                     containing forward and reverse primer sequences;
                     '>http://www.brachytag.org/vector-system.htm"
     misc_feature    7854..7878
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7879..7902
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             8106..8906
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccgctac gttagatcgt attctacagc ttccgtatca gccttgaata
     2641 gctctgcgtc cagtatctcg tgcagctaaa ggctacctca gtaagcgagt aacgcggccg
     2701 cttattaatt aagatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     2761 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     2821 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     2881 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     2941 tttattgcca aatgtttgaa cgatcgggga aattcgagct ctccggatcc cggtcggcat
     3001 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     3061 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     3121 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     3181 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     3241 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     3301 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     3361 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     3421 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     3481 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     3541 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     3601 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     3661 gcagcgatcg catccatggc ctccgcgacc ggctgcagtt atcatcatca tcatagacac
     3721 acgaaataaa gtaatcagat tatcagttaa agctatgtaa tatttacacc ataaccaatc
     3781 aattaaaaaa tagatcagtt taaagaaaga tcaaagctca aaaaaataaa aagagaaaag
     3841 ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt acctgcagaa cagcgggcag
     3901 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     3961 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     4021 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     4081 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     4141 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     4201 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
     4261 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     4321 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     4381 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     4441 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     4501 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     4561 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     4621 tagacgagag tgtcgtgctc caccatgttg acttaattaa aaggccgtac ggttaacgat
     4681 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
     4741 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
     4801 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
     4861 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
     4921 ttgaacgatc ggggaaattc gagctcggta cccggccgct ttacttgtac agctcgtcca
     4981 tgccgtgagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     5041 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     5101 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     5161 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     5221 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     5281 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     5341 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     5401 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     5461 ggctgaagca ctgcacgccg taggtgaagg tggtcacgag ggtgggccag ggcacgggca
     5521 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     5581 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     5641 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggatcctcta gagtcgacct
     5701 gcagaagtaa caccaaacaa cagggtgagc atcgacaaaa gaaacagtac caagcaaata
     5761 aatagcgtat gaaggcaggg ctaaaaaaat ccacatatag ctgctgcata tgccatcatc
     5821 caagtatatc aagatcaaaa taattataaa acatacttgt ttattataat agataggtac
     5881 tcaaggttag agcatatgaa tagatgctgc atatgccatc atgtatatgc atcagtaaaa
     5941 cccacatcaa catgtatacc tatcctagat cgatatttcc atccatctta aactcgtaac
     6001 tatgaagatg tatgacacac acatacagtt ccaaaattaa taaatacacc aggtagtttg
     6061 aaacagtatt ctactccgat ctagaacgaa tgaacgaccg cccaaccaca ccacatcatc
     6121 acaaccaagc gaacaaaaag catctctgta tatgcatcag taaaacccgc atcaacatgt
     6181 atacctatcc tagatcgata tttccatcca tcatcttcaa ttcgtaacta tgaatatgta
     6241 tggcacacac atacagatcc aaaattaata aatccaccag gtagtttgaa acagaattaa
     6301 ttctactccg atctagaacg accgcccaac cagaccacat catcacaacc aagacaaaaa
     6361 aaagcatgaa aagatgaccc gacaaacaag tgcacggcat atattgaaat aaaggaaaag
     6421 ggcaaaccaa accctatgca acgaaacaaa aaaaatcatg aaatcgatcc cgtctgcgga
     6481 acggctagag ccatcccagg attccccaaa gagaaacact ggcaagttag caatcagaac
     6541 gtgtctgacg tacaggtcgc atccgtgtac gaacgctagc agcacggatc taacacaaac
     6601 acggatctaa cacaaacatg aacagaagta gaactaccgg gccctaacca tggaccggaa
     6661 cgccgatcta gagaaggtag agaggggggg ggggggagga cgagcggcgt accttgaagc
     6721 ggaggtgccg acgggtggat ttgggggaga tctggttgtg tgtgtgtgcg ctccgaacaa
     6781 cacgaggttg gggaaagagg gtgtggaggg ggtgtctatt tattacggcg ggcgaggaag
     6841 ggaaagcgaa ggagcggtgg gaaaggaatc ccccgtagct gccggtgccg tgagaggagg
     6901 aggaggccgc ctgccgtgcc ggctcacgtc tgccgctccg ccacgcaatt tctggatgcc
     6961 gacagcggag caagtccaac ggtggagcgg aactctcgag aggggtccag aggcagcgac
     7021 agagatgccg tgccgtctgc ttcgcttggc ccgacgcgac gctgctggtt cgctggttgg
     7081 tgtccgttag actcgtcgac ggcgtttaac aggctggcat tatctactcg aaacaagaaa
     7141 aatgtttcct tagttttttt aatttcttaa agggtatttg tttaattttt agtcacttta
     7201 ttttattcta ttttatatct aaattattaa ataaaaaaac taaaatagag ttttagtttt
     7261 cttaatttag aggctaaaat agaataaaat agatgtacta aaaaaattag tctataaaaa
     7321 ccattaaccc taaaccctaa atggatgtac taataaaatg gatgaagtat tatataggtg
     7381 aagctatttg caaaaaaaaa ggagaacaca tgcacactaa aaagataaaa ctgtagagtc
     7441 ctgttgtcaa aatactcaat tgtcctttag accatgtcta actgttcatt tatatgattc
     7501 tctaaaacac tgatattatt gtagtactat agattatatt attcgtagag taaagtttaa
     7561 atatatgtat aaagatagat aaactgcact tcaaacaagt gtgacaaaaa aaatatgtgg
     7621 taatttttta taacttagac atgcaatgct cattatctct agagaggggc acgaccgggt
     7681 cacgctgcac tgcaggaatt caagctggca tcgagattca actagtctcg agtcgacagg
     7741 cctttctaac ctgtcgagga tacgtgataa gcgggagctt agcggagctc gttgcaatca
     7801 aagctcgata cgtcgagact tagaggatct acgacagtcc acagaacagg ccttgacagg
     7861 atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgtttaaacc cgggaggctc
     7921 gcggcggacg cacgacgccg gggcgagacc ataggcgatc tcctaaatca atagtagctg
     7981 taacctcgaa gcgtttcact tgtaacaacg attgagaatt tttgtcataa aattgaaata
     8041 cttggttcgc atttttgtca tccgcggtca gccgcaattc tgacgaactg cccatttagc
     8101 tggagatgat tgtacatcct tcacgtgaaa atttctcaag cgctgtgaac aagggttcag
     8161 attttagatt gaaaggtgag ccgttgaaac acgttcttct tgtcgatgac gacgtcgcta
     8221 tgcggcatct tattattgaa taccttacga tccacgcctt caaagtgacc gcggtagccg
     8281 acagcaccca gttcacaaga gtactctctt ccgcgacggt cgatgtcgtg gttgttgatc
     8341 taaatttagg tcgtgaagat gggctcgaga tcgttcgtaa tctggcggca aagtctgata
     8401 ttccaatcat aattatcagt ggcgaccgcc ttgaggagac ggataaagtt gttgcactcg
     8461 agctaggagc aagtgatttt atcgctaagc cgttcagtat cagagagttt ctagcacgca
     8521 ttcgggttgc cttgcgcgtg cgccccaacg ttgtccgctc caaagaccga cggtcttttt
     8581 gttttactga ctggacactt aatctcaggc aacgtcgctt gatgtccgaa gctggcggtg
     8641 aggtgaaact tacggcaggt gagttcaatc ttctcctcgc gtttttagag aaaccccgcg
     8701 acgttctatc gcgcgagcaa cttctcattg ccagtcgagt acgcgacgag gaggtttatg
     8761 acaggagtat agatgttctc attttgaggc tgcgccgcaa acttgaggcg gatccgtcaa
     8821 gccctcaact gataaaaaca gcaagaggtg ccggttattt ctttgacgcg gacgtgcagg
     8881 tttcgcacgg ggggacgatg gcagcctgag ccaattgcat ttgcctctta attatctggc
     8941 tcaaagggtg actgaggagt aagcgatgtg cccatcacac tgcccgggca tgcaagctga
     9001 tctggatct
//
