LOCUS       62056_4120        7273 bp DNA     circular SYN 18-NOV-2020
DEFINITION  Cloning vector pBluescript II KS(+) EACMCV DNA-A 1.6mer, complete 
            sequence.
ACCESSION   MT856195
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Deeply Sequenced Infectious Clones of Key Cassava Begomovirus 
            Isolates from Cameroon
  JOURNAL   Microbiol Resour Announc 9 (46), e00802-20 (2020)
  PUBMED    33184153
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2020) Ecology, Evolution, and Natural Resources, 
            Rutgers University, 14 College Farm Rd, New Brunswick, NJ 08901, USA
REFERENCE   3  (bases 1 to 7273)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc"; date: "2020"; volume: "9"; issue: "46"; pages: 
            "e00802-20"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-AUG-2020) Ecology, Evolution, and Natural Resources, Rutgers 
            University, 14 College Farm Rd, New Brunswick, NJ 08901, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Benchling v. 2019-01-22
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..861)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(862..966)
                     /label=AmpR promoter
     rep_origin      complement(992..1447)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1589..1605
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1666..1682
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2299..5098
                     /label=EACMCV DNA-A monomer unit, nick site to nick site
                     /note="EACMCV DNA-A monomer unit, nick site to nick site"
     gene            2471..2818
                     /gene="AV2"
                     /label=AV2
     CDS             2471..2818
                     /codon_start=1
                     /transl_table=11
                     /gene="AV2"
                     /product="AV2"
                     /label=AV2
                     /protein_id="QNR00655.1"
                     /translation="MWNPLVNDFPETVHGFRSMLAVKYLLHLEQEYDRGTVGAEYIRDL
                     IGVLRCKSYGEATRRYNNLNTRIQGAEEAELRQPIHEPCGCPYCPRHQKQNMGQQAHVS
                     EAQDVQDVSKP"
     gene            2631..3404
                     /gene="AV1"
                     /label=AV1
     CDS             2631..3404
                     /codon_start=1
                     /transl_table=11
                     /gene="AV1"
                     /product="CP"
                     /label=AV1
                     /protein_id="QNR00654.1"
                     /translation="MAKRPGDIIISTPVSKVRRRLNFDSPYTNRVVAPTVRVTRSKIWA
                     NRPMYRKPKMYRMYRSPDVPKGCEGPCKVQSYEQRDDVKHTGIVRCVSEVTRGSGITHR
                     VGKRLCVKSIYILGKIWMDENIKKQNHTNHVMFFLVRDRRPYGPSPQDFGQVFNMFDNE
                     PTTATVKNDYRDRFQVLRKFHATVVGGPSGMKEQALVKRFFRINNHIVYNHQEQAKYEN
                     HTENALLLYMACTHASNPVYATLKIRIYFYDAVTN"
     misc_feature    3226..4477
                     /note="unique part of EACMCV DNA-A partial tandem dimer,
                     HindIII to BamHI, noninclusive"
     gene            complement(3401..3805)
                     /gene="AC3"
                     /label=AC3
     CDS             complement(3401..3805)
                     /codon_start=1
                     /transl_table=11
                     /gene="AC3"
                     /product="REn"
                     /label=AC3
                     /protein_id="QNR00659.1"
                     /translation="MDLRTGEDIGALQWRSGAFIWEIPNPLYFTILNHDSMPFNMTHDI
                     IDVQIRFNYNLRQALGMHKCFLNFRIWTRLHPQTWRFFRTFRTQVMKYLTNLGVISIST
                     VIDAVHHVLNVVFVGTLSVSQDHAIKFNIY"
     gene            complement(3546..3953)
                     /gene="AC2"
                     /label=AC2
     CDS             complement(3546..3953)
                     /codon_start=1
                     /transl_table=11
                     /gene="AC2"
                     /product="TrAP"
                     /label=AC2
                     /protein_id="QNR00658.1"
                     /translation="MRSSSPSQSHSSPPPIKARHRQAKLRAPRRRRIDLTCGCSIYRSI
                     NCHNHGFTHRGRHWCSSMEEWRLYLGDSKSPIFHNPQPRQHAVQHDSRHNRCPDSVQLQ
                     PEASTGDAQVFSQLPDLDSFTSSDLAFLQNL"
     gene            complement(3883..4941)
                     /gene="AC1"
                     /label=AC1
     CDS             complement(3883..4941)
                     /codon_start=1
                     /transl_table=11
                     /gene="AC1"
                     /product="Rep"
                     /label=AC1
                     /protein_id="QNR00656.1"
                     /translation="MPRAGRFQINAKNYFITYPRCSLAKEEALSQLKAISYPTNIKFIR
                     VCRELHQDGVPHLHVLIQFEGKFQCTNPRFFDLISPSRSTHFHPNIQGAKSSSDVKAYI
                     EKGGEFLDDGIFQVDARSARGEGQHLAQVYAEALNASSKSEALQIIKEKDPKSFFLQFH
                     NISANADRIFQAPPQTYVSPFLSSSFTQVPEEIEVWVSENICRPAARPWRPISIVLEGD
                     SRTGKTMWARSLGPHNYLCGHLDLSPKIYSNDAWYNVIDDVDPHYLKHFKEFMGAQRDW
                     QSNTKYGKPIQIKGGIPTIFLCNPGPNSSYKEYLDEDKNSNLKNWAIKNALFISLTEPL
                     FSSTDQSQAQAS"
     misc_feature    4478..6025
                     /note="duplicated part of EACMCV DNA-A partial tandem
                     dimer, BamHI to HindIII, inclusive, copy 2"
     gene            complement(4551..4784)
                     /gene="AC4"
                     /label=AC4
     CDS             complement(4551..4784)
                     /codon_start=1
                     /transl_table=11
                     /gene="AC4"
                     /product="AC4"
                     /label=AC4
                     /protein_id="QNR00657.1"
                     /translation="MGCLISMFSSNSKASSNVPTPDSSISFPHPDQHISIRTFRELNHR
                     PMSRLTLKREGNFLTMEFSKSMPEVPGGRASI"
     primer_bind     complement(6028..6044)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6074..6092)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6113..6129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6137..6153)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6161..6191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6206..6227)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6515..7103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     1021 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1081 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1141 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1201 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1261 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     1321 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     1381 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     1441 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     1501 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     1561 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     1621 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
     1681 tccttttctt tgataatttg aagagcttct gatttagaag aagcattcaa cgcttctgca
     1741 tatacctgag ctaaatgctg gccctccccc ctggcacttc tggcatcgac ttggaaaatt
     1801 ccatcgtcaa gaaattcccc tcccttttca atgtaagcct tgacatcgga cgatgattta
     1861 gctccctgaa tgttcggatg gaaatgtgtt gatcgggatg gggaaatgag atcgaagaat
     1921 ctggggttgg tacattggaa cttgccttcg aattggatga gaacatggag atgaggcacc
     1981 ccatcctgat gtagttctct gcaaacccta atgaatttga tattcgtcgg gtaagaaatg
     2041 gcttttaatt gggaaagggc ctcttccttt gctaaggagc atcggggata ggttatgaaa
     2101 taatttttgg catttatttg aaaacgaccg gctctcggca tattggctgt cgttttgtat
     2161 cgggggacac tcaaaactcc aggggaacgg tggaatgggg ggcaatatat atgatgtccc
     2221 ccaatggcat atgtgtaaat aggtagactt ccattcaaaa tttgaattgc gaatattggc
     2281 ggccatccga ttaatattac cggatggccg cgcccgaaaa aagcaggtgg gccccatgat
     2341 agtccgcgtc cgcaaaagaa agtggtcccc gcgcacgcat gtttgtcggc caataatagt
     2401 tgcgccttga atgctagata ttgagttttg actttatata cttcgtcacg aagtagtgga
     2461 gcgcgtcaaa atgtggaatc cgttagttaa cgattttccg gaaaccgttc acggtttccg
     2521 ttcgatgctt gctgttaaat acctgttaca tctggaacag gaatatgatc gcggtactgt
     2581 cggggcggag tatatacgtg atctaatagg ggtgctacgg tgtaagagtt atggcgaagc
     2641 gaccaggaga tataataatc tcaacacccg tatccaaggt gcggaggagg ctgaacttcg
     2701 acagcccata cacgaaccgt gtggttgccc ctactgtccg cgtcaccaga agcaaaatat
     2761 gggccaacag gcccatgtat cggaagccca agatgtacag gatgtatcga agccctgacg
     2821 tccctaaggg ctgtgaaggc ccatgtaagg ttcagtctta tgaacagaga gatgatgtta
     2881 agcatactgg tatagtccga tgtgtcagtg aagttacgcg tgggtcaggc attacccata
     2941 gagttgggaa gaggttgtgt gtgaagtcca tatatatatt gggcaagatc tggatggatg
     3001 agaatatcaa gaagcaaaat catacgaacc atgttatgtt ttttctcgtt cgagatagaa
     3061 ggccttatgg gccgagtcct caagattttg gacaagtgtt caacatgttt gataatgaac
     3121 ctacgactgc aactgtgaag aatgattata gggaccggtt tcaggtgtta cgtaaattcc
     3181 atgcgactgt agttggtgga ccctcaggga tgaaggaaca agctttggtt aagaggtttt
     3241 ttaggatcaa taatcatata gtgtataatc atcaggaaca ggccaagtat gagaatcata
     3301 ccgagaatgc gttgttattg tatatggcat gtacacatgc ctcaaatcct gtgtacgcta
     3361 cgcttaaaat acgcatctat ttctatgatg cagtgacaaa ttaataaata ttaaatttga
     3421 ttgcatgatc ttgggataca gatagggttc ccacaaaaac tacattcaat acatgatgca
     3481 ctgcatctat tacagttgaa atcgaaataa cacctaaatt agtgagatat ttcatgactt
     3541 gtgtcctaaa ggttctgaag aaacgccaag tctgaggatg taaacgagtc cagatccgga
     3601 agttgagaaa acacttgtgc atccccagtg cttgcctcag gttgtagttg aaccgaatct
     3661 ggacatctat tatgtcgtga gtcatgttga acggcatgct gtcgtggttg aggattgtga
     3721 aatatagggg atttggaatc tcccagataa aggcgccact cctccattga agagcaccaa
     3781 tgtcttcccc tgtgcgtaaa tccatggttg tgacaattaa tgctgcgata gatggaacag
     3841 ccacaagtta ggtcgatgcg tcgacgtcta ggtgctctga gcttagcttg cctgtgcctg
     3901 gctttgatcg gtggaggaga agagtggctc tgtgagggag atgaagagcg cattcttgat
     3961 tgcccaattc ttgagattgg aattcttgtc ctcgtctagg tattctttat aggacgaatt
     4021 ggggcccgga ttgcataaga agatggtggg aatcccacct ttaatttgaa tgggctttcc
     4081 gtattttgtg tttgattgcc aatctcgttg ggcccccatg aattctttga aatgctttag
     4141 ataatgcggg tctacgtcgt caatgacgtt gtaccatgcg tcgtttgaat atatcttggg
     4201 agacagatcc aggtgtccac atagatagtt atgtgggccc agagatcggg cccacatcgt
     4261 cttcccggtt cggctatcac cttcgagaac aatactgatc ggtctccatg gccgcgcagc
     4321 gggacggcat atattttcgg atacccatac ttctatttcc tctgggactt gcgtaaatga
     4381 ggatgataag aacggactaa cgtaagtttg tggcggagcc tggaagattc gatccgcgtt
     4441 agcagatatg ttatggaact gtaaaaaaaa ggactttgga tccttttctt tgataatttg
     4501 aagagcttct gatttagaag aagcattcaa cgcttctgca tatacctgag ctaaatgctg
     4561 gccctccccc ctggcacttc tggcatcgac ttggaaaatt ccatcgtcaa gaaattcccc
     4621 tcccttttca atgtaagcct tgacatcgga cgatgattta gctccctgaa tgttcggatg
     4681 gaaatgtgtt gatcgggatg gggaaatgag atcgaagaat ctggggttgg tacattggaa
     4741 cttgccttcg aattggatga gaacatggag atgaggcacc ccatcctgat gtagttctct
     4801 gcaaacccta atgaatttga tattcgtcgg gtaagaaatg gcttttaatt gggaaagggc
     4861 ctcttccttt gctaaggagc atcggggata ggttatgaaa taatttttgg catttatttg
     4921 aaaacgaccg gctctcggca tattggctgt cgttttgtat cgggggacac tcaaaactcc
     4981 aggggaacgg tggaatgggg ggcaatatat atgatgtccc ccaatggcat atgtgtaaat
     5041 aggtagactt ccattcaaaa tttgaattgc gaatattggc ggccatccga ttaatattac
     5101 cggatggccg cgcccgaaaa aagcaggtgg gccccatgat agtccgcgtc cgcaaaagaa
     5161 agtggtcccc gcgcacgcat gtttgtcggc caataatagt tgcgccttga atgctagata
     5221 ttgagttttg actttatata cttcgtcacg aagtagtgga gcgcgtcaaa atgtggaatc
     5281 cgttagttaa cgattttccg gaaaccgttc acggtttccg ttcgatgctt gctgttaaat
     5341 acctgttaca tctggaacag gaatatgatc gcggtactgt cggggcggag tatatacgtg
     5401 atctaatagg ggtgctacgg tgtaagagtt atggcgaagc gaccaggaga tataataatc
     5461 tcaacacccg tatccaaggt gcggaggagg ctgaacttcg acagcccata cacgaaccgt
     5521 gtggttgccc ctactgtccg cgtcaccaga agcaaaatat gggccaacag gcccatgtat
     5581 cggaagccca agatgtacag gatgtatcga agccctgacg tccctaaggg ctgtgaaggc
     5641 ccatgtaagg ttcagtctta tgaacagaga gatgatgtta agcatactgg tatagtccga
     5701 tgtgtcagtg aagttacgcg tgggtcaggc attacccata gagttgggaa gaggttgtgt
     5761 gtgaagtcca tatatatatt gggcaagatc tggatggatg agaatatcaa gaagcaaaat
     5821 catacgaacc atgttatgtt ttttctcgtt cgagatagaa ggccttatgg gccgagtcct
     5881 caagattttg gacaagtgtt caacatgttt gataatgaac ctacgactgc aactgtgaag
     5941 aatgattata gggaccggtt tcaggtgtta cgtaaattcc atgcgactgt agttggtgga
     6001 ccctcaggga tgaaggaaca agcttatcga taccgtcgac ctcgaggggg ggcccggtac
     6061 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     6121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccgggagca
     6181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6301 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7261 cttggtctga cag
//
