LOCUS       V009150                 7143 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009150
VERSION     V009150
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7143)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7143)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             215..2287
                     /gene="Gucy1a1"
                     /label="Guanylate cyclase soluble subunit alpha-1"
                     /note="Guanylate cyclase soluble subunit alpha-1 from Mus
                     musculus. Accession#: Q9ERL9"
     CDS             2312..2353
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2363..2380
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2405..2537
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_recomb     2550..3255
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        3828..3932
                     /label="AmpR promoter"
     CDS             3933..4790
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4964..5552
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5729..6841)
                     /codon_start=1
                     /note="unnamed protein product; 5' lacZ fragment"
                     /protein_id="SJL87790.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6842..7116)
                     /label="AcNPV-ETL promoter"
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
      181 tgcccttaag ccactgccaa gctccaggaa caccatgttc tgcaggaagt tcaaggatct
      241 caagatcaca ggggagtgtc ctttctcctt actggcccct ggtcaggttc ctaaggagcc
      301 aacagaggag gtggctggag gctctgaggg ctgccaggct actctgccca tctgccagta
      361 ctttcctgag aagaatgcag aagggagtct cccccaaaga aagacaagcc gcaacagagt
      421 ctacctgcac accctggcag agagtatttg caagctcatc ttcccagagt gtgagcgact
      481 gaaccttgca cttcagagaa ccttggcaaa gcataaaata gaagaaaaca ggaaatcttc
      541 agaaaaagaa gaccttgaaa aaataatcgc agaagaagca attgcagcag gtgccccagt
      601 ggaggcgctc aaagactctc tgggcgagga gctgttcaag atctgctatg aggaagatga
      661 gcacattttg ggcgtggttg gcggcaccct gaaggacttt ctaaatagct tcagcacgct
      721 cctcaagcag agcagccact gccaagaggc ggagaggcgg ggacgactgg aagatgcctc
      781 catcttatgc ctggacaagg accaggactt tctaaatgtt tactacttct tcccgaagag
      841 aaccacagcc ctgcttctcc ctggtatcat taaagcggct gctcgcatac tgtacgaaag
      901 ccacgtggag gtgtccctga tgcctccctg cttccgaagt gactgtaccg agtttgtgaa
      961 ccagccctat ttgctctact ccgttcatgt gaagagcacc aagccgtccc tgtccccagg
     1021 caagccccag tcctcgctag tgatccccgc ttcgctcttc tgcaagactt tcccgttcca
     1081 tttcatgctg gaccgagacc tggccatcct gcagctgggt aacggcatca gaaggctggt
     1141 gaacaagagg gacttccaag ggaagcccaa ctttgaagag ttctttgaaa ttctaactcc
     1201 caaaatcaac cagacattta gtggcatcat gacaatgttg aacatgcagt ttgtcatccg
     1261 ggtgaggaga tgggataact cggtgaagaa atcgtcaagg gttatggatc tcaaaggtca
     1321 aatgatctac atcgttgaat ccagtgccat cttgttctta gggtcaccat gtgtggacag
     1381 gctggaagat ttcacaggac gggggctcta tctgtccgac atcccaattc ataacgccct
     1441 gagggatgtt gtcttgatag gggagcaggc acgggctcaa gatggcctca agaagaggtt
     1501 ggggaagctg aaggcaaccc tggagcatgc ccaccaagcc ctggaggaag agaagaagag
     1561 gacagtggat ctgctgtgct ctatcttccc ctctgaggtt gctcagcagc tgtggcaagg
     1621 acaaattgtg caagccaaga aattcagcga ggtcaccatg cttttctcag atatcgtagg
     1681 gttcactgct atctgctctc agtgttcacc tctgcaggtc atcacgatgc tcaacgctct
     1741 ctacactcgc tttgaccagc agtgtggaga gctggatgtc tacaaggtgg agaccatcgg
     1801 ggatgcatat tgtgtggcag gtggattgca cagagagagt gacacccatg ctgtccagat
     1861 agcactgatg gccctgaaga tgatggagct ctccaatgag gtcatgtctc cccacggaga
     1921 acctatcaag atgcgaattg gactacattc tggatcagtg tttgctggag ttgtcggagt
     1981 gaagatgccc cggtattgcc tgtttggaaa caatgtcact ctggctaaca aatttgaatc
     2041 ctgcagtgtg cctcggaaaa tcaatgtcag ccccaccaca tacaggttac tcaaagactg
     2101 tcctggcttt gtgttcaccc cgagatcaag ggaggagctt ccaccaaact tccctagtga
     2161 cattcctggg atctgtcact ttctggatgc ttatcaccat caaggaccta attccaaacc
     2221 atggttccag gataaagatg tggaagatgg aaacgccaac ttcttaggca aagcgtcagg
     2281 ggtagataag ggcaattcga agcttaggcc tggtaagcct atccctaacc ctctcctcgg
     2341 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgacgtctct agcttggagt
     2401 cgacaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2521 atcttatcat ctctcgactc tgctgaagag gaggaaattc tccttgaagt ttccctggtg
     2581 ttcaaagtaa aggagtttgc accagacgca cctctgttca ctggtccggc gtattaaaac
     2641 acgatacatt gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca
     2701 gacaattgtt gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt
     2761 agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca
     2821 atagatttgt aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg
     2881 gctggactat ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc
     2941 aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc
     3001 aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac
     3061 gtaaacacgt taaataaagc ctggacatat ttaacatcgg gcgtgttagc tttattaggc
     3121 cgattatcgt cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc
     3181 atagccacac gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt
     3241 gagctttttg gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg
     3301 cccgatttta attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca
     3361 gacggcaaat ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga
     3421 ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc
     3481 ggtttaggct caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt
     3541 tcgggcgatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     3601 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3661 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3721 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3781 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3901 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4021 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4081 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4141 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4201 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4261 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4321 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4381 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4441 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4501 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4561 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5101 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5701 agcgcagcga gtcagtgagc gaggaagcat cctgcaccat cgtctgctca tccatgacct
     5761 gaccatgcag aggatgatgc tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt
     5821 tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg
     5881 cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag agattcggga
     5941 tttcggcgct ccacagtttc gggttttcga cgttcagacg tagtgtgacg cgatcggcat
     6001 aaccaccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg
     6061 tttcaccctg ccataaagaa actgttaccc gtaggtagtc acgcaactcg ccgcacatct
     6121 gaacttcagc ctccagtaca gcgcggctga aatcatcatt aaagcgagtg gcaacatgga
     6181 aatcgctgat ttgtgtagtc ggtttatgca gcaacgagac gtcacggaaa atgccgctca
     6241 tccgccacat atcctgatct tccagataac tgccgtcact ccagcgcagc accatcaccg
     6301 cgaggcggtt ttctccggcg cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac
     6361 tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc
     6421 catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaaca ttaaatgtga
     6481 gcgagtaaca acccgtcgga ttctccgtgg gaacaaacgg cggattgacc gtaatgggat
     6541 aggtcacgtt ggtgtagatg ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga
     6601 cgacagtatc ggcctcagga agatcgcact ccagccagct ttccggcacc gcttctggtg
     6661 ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6721 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6781 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg ggatctatca
     6841 tttttagcag tgattctaat tgcagctgct ctttgataca actaatttta cgacgacgat
     6901 gcgagctttt attcaaccga gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc
     6961 attatcgtat tgttgcacat caacaggctg gacaccacgt tgaactcgcc gcagttttgc
     7021 ggcaagttgg acccgccgcg catccaatgc aaactttccg acattctgtt gcctacgaac
     7081 gattgattct ttgtccattg atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg
     7141 ctt
//
