LOCUS       V009151                 7173 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009151
VERSION     V009151
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             213..2285
                     /gene="Gucy1a1"
                     /label="Guanylate cyclase soluble subunit alpha-1"
                     /note="Guanylate cyclase soluble subunit alpha-1 from Mus
                     musculus. Accession#: Q9ERL9"
     CDS             2342..2383
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2393..2410
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2435..2567
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_recomb     2580..3285
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        3858..3962
                     /label="AmpR promoter"
     CDS             3963..4820
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4994..5582
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5759..6871)
                     /codon_start=1
                     /note="unnamed protein product; 5' lacZ fragment"
                     /protein_id="SJL87794.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6872..7146)
                     /label="AcNPV-ETL promoter"
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
      181 tgcccttgcc actgccaagc tccaggaaca ccatgttctg caggaagttc aaggatctca
      241 agatcacagg ggagtgtcct ttctccttac tggcccctgg tcaggttcct aaggagccaa
      301 cagaggaggt ggctggaggc tctgagggct gccaggctac tctgcccatc tgccagtact
      361 ttcctgagaa gaatgcagaa gggagtctcc cccaaagaaa gacaagccgc aacagagtct
      421 acctgcacac cctggcagag agtatttgca agctcatctt cccagagtgt gagcgactga
      481 accttgcact tcagagaacc ttggcaaagc ataaaataga agaaaacagg aaatcttcag
      541 aaaaagaaga ccttgaaaaa ataatcgcag aagaagcaat tgcagcaggt gccccagtgg
      601 aggcgctcaa agactctctg ggcgaggagc tgttcaagat ctgctatgag gaagatgagc
      661 acattttggg cgtggttggc ggcaccctga aggactttct aaatagcttc agcacgctcc
      721 tcaagcagag cagccactgc caagaggcgg agaggcgggg acgactggaa gatgcctcca
      781 tcttatgcct ggacaaggac caggactttc taaatgttta ctacttcttc ccgaagagaa
      841 ccacagccct gcttctccct ggtatcatta aagcggctgc tcgcatactg tacgaaagcc
      901 acgtggaggt gtccctgatg cctccctgct tccgaagtga ctgtaccgag tttgtgaacc
      961 agccctattt gctctactcc gttcatgtga agagcaccaa gccgtccctg tccccaggca
     1021 agccccagtc ctcgctagtg atccccgctt cgctcttctg caagactttc ccgttccatt
     1081 tcatgctgga ccgagacctg gccatcctgc agctgggtaa cggcatcaga aggctggtga
     1141 acaagaggga cttccaaggg aagcccaact ttgaagagtt ctttgaaatt ctaactccca
     1201 aaatcaacca gacatttagt ggcatcatga caatgttgaa catgcagttt gtcatccggg
     1261 tgaggagatg ggataactcg gtgaagaaat cgtcaagggt tatggatctc aaaggtcaaa
     1321 tgatctacat cgttgaatcc agtgccatct tgttcttagg gtcaccatgt gtggacaggc
     1381 tggaagattt cacaggacgg gggctctatc tgtccgacat cccaattcat aacgccctga
     1441 gggatgttgt cttgataggg gagcaggcac gggctcaaga tggcctcaag aagaggttgg
     1501 ggaagctgaa ggcaaccctg gagcatgccc accaagccct ggaggaagag aagaagagga
     1561 cagtggatct gctgtgctct atcttcccct ctgaggttgc tcagcagctg tggcaaggac
     1621 aaattgtgca agccaagaaa ttcagcgagg tcaccatgct tttctcagat atcgtagggt
     1681 tcactgctat ctgctctcag tgttcacctc tgcaggtcat cacgatgctc aacgctctct
     1741 acactcgctt tgaccagcag tgtggagagc tggatgtcta caaggtggag accatcgggg
     1801 atgcatattg tgtggcaggt ggattgcaca gagagagtga cacccatgct gtccagatag
     1861 cactgatggc cctgaagatg atggagctct ccaatgaggt catgtctccc cacggagaac
     1921 ctatcaagat gcgaattgga ctacattctg gatcagtgtt tgctggagtt gtcggagtga
     1981 agatgccccg gtattgcctg tttggaaaca atgtcactct ggctaacaaa tttgaatcct
     2041 gcagtgtgcc tcggaaaatc aatgtcagcc ccaccacata caggttactc aaagactgtc
     2101 ctggctttgt gttcaccccg agatcaaggg aggagcttcc accaaacttc cctagtgaca
     2161 ttcctgggat ctgtcacttt ctggatgctt atcaccatca aggacctaat tccaaaccat
     2221 ggttccagga taaagatgtg gaagatggaa acgccaactt cttaggcaaa gcgtcagggg
     2281 tagattagtg agccacatgc tcttatgttt gatgcctaag ggcaattcga agcttaggcc
     2341 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     2401 ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag cttataatgg
     2461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc tgctgaagag
     2581 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca
     2641 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa
     2701 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
     2761 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
     2821 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
     2881 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
     2941 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     3001 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     3061 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ctggacatat
     3121 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     3181 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
     3241 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
     3301 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
     3361 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
     3421 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
     3481 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
     3541 acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc agtacaatct
     3601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcat
     5761 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt
     5821 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca
     5881 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca
     5941 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga
     6001 cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc
     6061 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc
     6121 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga
     6181 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca
     6241 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac
     6301 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg
     6361 cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt
     6421 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct
     6481 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg
     6541 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt
     6601 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact
     6661 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat
     6721 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6781 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6841 cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat tgcagctgct
     6901 ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga gcgtgcatgt
     6961 ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat caacaggctg
     7021 gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg catccaatgc
     7081 aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg atcgaagcga
     7141 gtgccttcga ctttttcgtg tccagtgtgg ctt
//
