LOCUS       V009152                 7123 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009152
VERSION     V009152
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7123)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7123)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             191..2260
                     /gene="GUCY1A1"
                     /label="Guanylate cyclase soluble subunit alpha-1"
                     /note="Guanylate cyclase soluble subunit alpha-1 from Homo
                     sapiens. Accession#: Q02108"
     CDS             2292..2333
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2343..2360
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2385..2517
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_recomb     2530..3235
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        3808..3912
                     /label="AmpR promoter"
     CDS             3913..4770
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4944..5532
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5709..6821)
                     /codon_start=1
                     /note="unnamed protein product; 5' lacZ fragment"
                     /protein_id="SJL87809.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6822..7096)
                     /label="AcNPV-ETL promoter"
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
      181 tgcccttacc atgttctgca cgaagctcaa ggatctcaag atcacaggag agtgtccttt
      241 ctccttactg gcaccaggtc aagttcctaa cgagtcttca gaggaggcag caggaagctc
      301 agagagctgc aaagcaaccg tgcccatctg tcaagacatt cctgagaaga acatacaaga
      361 aagtcttcct caaagaaaaa ccagtcggag ccgagtctat cttcacactt tggcagagag
      421 tatttgcaaa ctgattttcc cagagtttga acggctgaat gttgcacttc agagaacatt
      481 ggcaaagcac aaaataaaag aaagcaggaa atctttggaa agagaagact ttgaaaaaac
      541 aattgcagag caagcagttg cagcaggagt tccagtggag gttatcaaag aatctcttgg
      601 tgaagaggtt tttaaaatat gttacgagga agatgaaaac atccttgggg tggttggagg
      661 cacccttaaa gattttttaa acagcttcag tacccttctg aaacagagca gccattgcca
      721 agaagcagga aaaaggggca ggcttgagga cgcctccatt ctatgcctgg ataaggagga
      781 tgattttcta catgtttact acttcttccc taagagaacc acctccctga ttcttcccgg
      841 catcataaag gcagctgctc acgtattata tgaaacggaa gtggaagtgt cgttaatgcc
      901 tccctgcttc cataatgatt gcagcgagtt tgtgaatcag ccctacttgt tgtactccgt
      961 tcacatgaaa agcaccaagc catccctgtc ccccagcaaa ccccagtcct cgctggtgat
     1021 tcccacatcg ctattctgca agacatttcc attccatttc atgtttgaca aagatatgac
     1081 aattctgcaa tttggcaatg gcatcagaag gctgatgaac aggagagact ttcaaggaaa
     1141 gcctaatttt gaagaatact ttgaaattct gactccaaaa atcaaccaga cgtttagcgg
     1201 gatcatgact atgttgaata tgcagtttgt tgtacgagtg aggagatggg acaactctgt
     1261 gaagaaatct tcaagggtta tggacctcaa aggccaaatg atctacattg ttgaatccag
     1321 tgcaatcttg tttttggggt caccctgtgt ggacagatta gaagatttta caggacgagg
     1381 gctctacctc tcagacatcc caattcacaa tgcactgagg gatgtggtct taatagggga
     1441 acaagcccga gctcaagatg gcctgaagaa gaggctgggg aagctgaagg ctacccttga
     1501 gcaagcccac caagccctgg aggaggagaa gaaaaagaca gtagaccttc tgtgctccat
     1561 atttccctgt gaggttgctc agcagctgtg gcaagggcaa gttgtgcaag ccaagaagtt
     1621 cagtaatgtc accatgctct tctcagacat cgttgggttc actgccatct gctcccagtg
     1681 ctcaccgctg caggtcatca ccatgctcaa tgcactgtac actcgcttcg accagcagtg
     1741 tggagagctg gatgtctaca aggtggagac cattggcgat gcctattgtg tagctggggg
     1801 attacacaaa gagagtgata ctcatgctgt tcagatagcg ctgatggccc tgaagatgat
     1861 ggagctctct gatgaagtta tgtctcccca tggagaacct atcaagatgc gaattggact
     1921 gcactctgga tcagtttttg ctggcgtcgt tggagttaaa atgccccgtt actgtctttt
     1981 tggaaacaat gtcactctgg ctaacaaatt tgagtcctgc agtgtaccac gaaaaatcaa
     2041 tgtcagccca acaacttaca gattactcaa agactgtcct ggtttcgtgt ttacccctcg
     2101 atcaagggag gaacttccac caaacttccc tagtgaaatc cccggaatct gccattttct
     2161 ggatgcttac caacaaggaa caaactcaaa accatgcttc caaaagaaag atgtggaaga
     2221 tggcaatgcc aattttttag gcaaagcatc aggaatagat tagcaacaag ggcaattcga
     2281 agcttaggcc tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     2341 gtcatcatca ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag
     2401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc
     2521 tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc
     2581 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta
     2641 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt
     2701 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt
     2761 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt
     2821 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt
     2881 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt
     2941 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt
     3001 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc
     3061 ctggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca
     3121 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt
     3181 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc
     3241 tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa
     3301 cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg
     3361 cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg
     3421 cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc
     3481 tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc
     3541 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5701 gaggaagcat cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc
     5761 tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt
     5821 tcaatccgca cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg
     5881 gcggtgtgca gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc
     5941 gggttttcga cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata
     6001 atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa
     6061 actgttaccc gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca
     6121 gcgcggctga aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc
     6181 ggtttatgca gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct
     6241 tccagataac tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg
     6301 cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc
     6361 cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc
     6421 tggccttcct gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga
     6481 ttctccgtgg gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg
     6541 ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga
     6601 agatcgcact ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc
     6661 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6721 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6781 ttttcccagt cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat
     6841 tgcagctgct ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga
     6901 gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat
     6961 caacaggctg gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg
     7021 catccaatgc aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg
     7081 atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg ctt
//
