LOCUS       40924_6716        4853 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..94
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             110..127
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             131..163
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             167..190
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_recomb     257..962
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1538..1642
                     /label=AmpR promoter
     CDS             1643..2500
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2674..3262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaacctat aaatatgccg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga
      181 tgacgataag gatccgagct cgagatctgc agctggtacc atggaattcg aagcttggag
      241 tcgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg
      301 agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt
      361 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta
      421 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca
      481 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa
      541 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta
      601 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt
      661 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg
      721 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa
      781 ataaagcctg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt
      841 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac
      901 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa
      961 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt
     1021 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta
     1081 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg
     1141 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa
     1201 attgtctctt tcaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgtatg
     1261 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     1321 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1381 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1441 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1501 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1561 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1621 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1681 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1741 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1801 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1861 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1921 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1981 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2041 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2101 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2161 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2221 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2281 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2341 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2401 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2461 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2521 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2581 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2641 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2701 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2761 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2821 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2881 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2941 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3001 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3061 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3421 gtcagtgagc gaggaagcat cctgcaccat cgtctgctca tccatgacct gaccatgcag
     3481 aggatgatgc tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt tcagcagcag
     3541 cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg cttcaatcag
     3601 cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag agattcggga tttcggcgct
     3661 ccacagtttc gggttttcga cgttcagacg tagtgtgacg cgatcggtat aaccaccacg
     3721 ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg
     3781 ccataaagaa actgttaccc gtaggtagtc acgcaactcg ccgcacatct gaacttcagc
     3841 ctccagtaca gcgcggctga aatcatcatt aaagcgagtg gcaacatgga aatcgctgat
     3901 ttgtgtagtc ggtttatgca gcaacgagac gtcacggaaa atgccgctca tccgccacat
     3961 atcctgatct tccagataac tgccgtcact ccaacgcagc accatcaccg cgaggcggtt
     4021 ttctccggcg cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc
     4081 gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc catcaaaaat
     4141 aattcgcgtc tggccttcct gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca
     4201 acccgtcgga ttctccgtgg gaacaaacgg cggattgacc gtaatgggat aggtcacgtt
     4261 ggtgtagatg ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga cgacagtatc
     4321 ggcctcagga agatcgcact ccagccagct ttccggcacc gcttctggtg ccggaaacca
     4381 ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4441 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     4501 aacgccaggg ttttcccagt cacgacgttg taaaacgacg ggatctatca tttttagcag
     4561 tgattctaat tgcagctgct ctttgataca actaatttta cgacgacgat gcgagctttt
     4621 attcaaccga gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat
     4681 tgttgcacat caacaggctg gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg
     4741 acccgccgcg catccaatgc aaactttccg acattctgtt gcctacgaac gattgattct
     4801 ttgtccattg atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg ctt
//
