LOCUS       40924_6711        4863 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..94
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             110..127
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             131..163
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             167..190
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_recomb     267..972
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1548..1652
                     /label=AmpR promoter
     CDS             1653..2510
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2684..3272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaacctat aaatatgccg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga
      181 tgacgataag gatcgatggg gatccgagct cgagatctgc agctggtacc atggaattcg
      241 aagcttggag tcgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc
      301 aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg
      361 atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
      421 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
      481 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
      541 gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct
      601 ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat
      661 ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa
      721 atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta
      781 aacacgttaa ataaagcctg gacatattta acatcgggcg tgttagcttt attaggccga
      841 ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata
      901 gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag
      961 ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc
     1021 gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac
     1081 ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc
     1141 gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt
     1201 ttaggctcaa attgtctctt tcaggcaaca cagtcggcac ctcaactatt gtactggttt
     1261 cgggcgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     1321 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     1381 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     1441 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1501 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3421 agcgcagcga gtcagtgagc gaggaagcat cctgcaccat cgtctgctca tccatgacct
     3481 gaccatgcag aggatgatgc tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt
     3541 tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg
     3601 cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag agattcggga
     3661 tttcggcgct ccacagtttc gggttttcga cgttcagacg tagtgtgacg cgatcggtat
     3721 aaccaccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg
     3781 tttcaccctg ccataaagaa actgttaccc gtaggtagtc acgcaactcg ccgcacatct
     3841 gaacttcagc ctccagtaca gcgcggctga aatcatcatt aaagcgagtg gcaacatgga
     3901 aatcgctgat ttgtgtagtc ggtttatgca gcaacgagac gtcacggaaa atgccgctca
     3961 tccgccacat atcctgatct tccagataac tgccgtcact ccaacgcagc accatcaccg
     4021 cgaggcggtt ttctccggcg cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac
     4081 tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc
     4141 catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaaca ttaaatgtga
     4201 gcgagtaaca acccgtcgga ttctccgtgg gaacaaacgg cggattgacc gtaatgggat
     4261 aggtcacgtt ggtgtagatg ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga
     4321 cgacagtatc ggcctcagga agatcgcact ccagccagct ttccggcacc gcttctggtg
     4381 ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4441 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4501 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg ggatctatca
     4561 tttttagcag tgattctaat tgcagctgct ctttgataca actaatttta cgacgacgat
     4621 gcgagctttt attcaaccga gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc
     4681 attatcgtat tgttgcacat caacaggctg gacaccacgt tgaactcgcc gcagttttgc
     4741 ggcaagttgg acccgccgcg catccaatgc aaactttccg acattctgtt gcctacgaac
     4801 gattgattct ttgtccattg atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg
     4861 ctt
//
