LOCUS       62056_4115        5756 bp DNA     circular SYN 30-SEP-2020
DEFINITION  Shuttle expression vector pBlu10-Htk, complete sequence.
ACCESSION   MN843970
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Le Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2019) School of Environment and Safety 
            Engineering, Jiangsu University, Xuefu Road 301, Zhen Jiang, Jiangsu
            212013, China
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2019) School of Environment and Safety Engineering, Jiangsu 
            University, Xuefu Road 301, Zhen Jiang, Jiangsu 212013, China"
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      130..2505
                     /label=Gram-positive origin of replication
                     /note="Gram-positive origin of replication"
     regulatory      2506..3047
                     /regulatory_class="promoter"
     gene            3048..3809
                     /gene="Htk"
                     /label=Htk
     CDS             3048..3809
                     /codon_start=1
                     /transl_table=11
                     /gene="Htk"
                     /product="kanamycin resistance protein"
                     /label=Htk
                     /protein_id="QOC69212.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     regulatory      3810..3832
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    3836..3865
                     /label=MCS
                     /note="MCS"
     regulatory      3866..3923
                     /regulatory_class="terminator"
     rep_origin      complement(4009..4597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4771..5628)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5629..5733)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg aattgacaaa gttttctatt tgtgttaaca ttgtttatat aatagtgaac
      181 agtgttaaga ttaaatgtga ggtgtttgta tggatattaa tgattataaa gagaagggac
      241 tttatttatt aagtagtatg gatgatttta ttaaaattaa tgatttgttt atgggtaaag
      301 ttgtttctcc tggctatgtt gcttcggttt ttggtgtttc caggtctact gttacacaat
      361 ggattcaaag acgtaaaatt agagctttta agtataaagg taaggaaggt gactatatgg
      421 ttatacctat tgctgatatt attgattaca aaagattgag taataatgat tttatttatg
      481 ataagttagt gaggtgattt attttatgtt tgacgatagc tatgttgtta atgagtgttc
      541 gtctaatgtt agtgaaaatg atagagattt ttgtagtttg gttggtcgtt ttatgattat
      601 taatggtata gataagttgg ttattaagat taatagaaaa tttaatagga aatctttaag
      661 tttagatttt agtgttgatt tattcccttc tatcaaagtt tctgaattag ttttttttga
      721 tgagtttaac aaaacgtgtg gtttttattt ttcttttaat tcttttacaa tttttaaggc
      781 ttttagagat gttcataatc ataataaaat atcattttat tttgcataat ttcgggtctg
      841 ggccgcagac caggcccagt gctaacaata ttaattttta atgttaggaa ttgtttaatt
      901 cttaattgtg tttttaaagg tagaataatt acccattcgc cctttagcca acaaaaatta
      961 aggaggtata aacatggata aaatggattt gattcttcaa gatgaaagac tgggtgagat
     1021 atttaaagat atagatttaa cagataatga aaagagatat cttaaatggt tatggaaatg
     1081 ggattatgaa acacgtgata cttttgtatc aatttttttg aagctaaaaa atggtggaaa
     1141 atgatttttt tcttatcttg atatattaga aaaaagcgta ctcacgaagt aagaatttgt
     1201 aaaaaaagaa ggggggattt ttttggatga gagtttgtac aagcagattt taagtaatat
     1261 tattattact cgtgattatt gtaaaaatgt tttagataat ataaagttca atgaaaaaat
     1321 aattgattat tatgttatgt tacaaaatga tgtttttatt gattttacta ataaaataaa
     1381 ttcaataagg gattgtaata aatattggta tttggatgtt tataaaaagc agaaaataaa
     1441 ggattttaaa aagactaatt tgtgtaaaga taagttctgt aataattgta agaaagttaa
     1501 acaggcttca agaatgcaaa aatatattcc tgaattacag aaatacaaag atggcttata
     1561 tcattttata tttactgttg aaaatgtgcc aggtagtgaa ttaagagata ctattgatag
     1621 gttgtttaag tcttttaagt catttacaag gtatttaagt ggtaatctta aaataaaagg
     1681 tgttaatttt gataaatggg gttataaagg ctgtgtaagg tctttagagg taacttatag
     1741 tatgattgat aatcatatta tgtatcatcc acacttgcat gttgcgatga tattagatcc
     1801 tttttacgat ggttttaatg ttgaaaggat gcatataatt aataagttta gttatagcta
     1861 tggtgtttta aaaaggttgt ttactgatga tgaattatta attcaaaaaa tttggtattt
     1921 attgtttaat aatattgagg ttaacatggc caatataaat aatttagagg atggttattc
     1981 ttgtttagtt aataagttta gtgattatga ttatgcggag ctgtttaagt atatttgtaa
     2041 aaatactgat gaacaaggtt tacttatgac ttatgatatt tttaaagatt tatattttgc
     2101 attacataat gttcatcaga tacaaggcta tggttgttta tataatataa gagatgatac
     2161 tcaattagat ttaaaggttg atgacattta taatgatttg attgatttat tacaagttac
     2221 agaaaatcct atacagtcta tggaaactgt acaggattta ttaaaggata ctgaatatac
     2281 aataataagc cgtaagcgta tatttaagta tctaacacaa ttatatcata aggattgata
     2341 tttataccgt ctgtcggact catgcggagg gggacttgag ggggtctccc ctcgcattgt
     2401 acgacagacg gtattattat tatacaaatt ttttttatgt aatttttttt gtgtaatttt
     2461 tttatacaaa taatatttca attcgtcgac ctgcagaagc ctggctgcag gtcgataaac
     2521 ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga
     2581 cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg
     2641 aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc
     2701 ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg
     2761 cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact aatgcttgaa
     2821 acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt caaaatcggc
     2881 tccgtcgata ctatgttata cgccaacttt caaaacaact ttgaaaaagc tgttttctgg
     2941 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
     3001 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg aaaggaccaa
     3061 taataatgac tagagaagaa agaatgaaga ttgttcatga aattaaggaa cgaatattgg
     3121 ataaatatgg ggatgatgtt aaggcaattg gtgtttatgg ctctcttggt cgtcagactg
     3181 atgggcccta ttcggatatt gagatgatgt gtgttctgtc aacagaggga gtagagttca
     3241 gctatgaatg gacaaccggt gagtggaagg cggaagtgaa tttttatagc gaagagattc
     3301 tactagatta tgcatctcgg gtggaaccgg attggccgct tacacatggt cgatttttct
     3361 ctattttgcc gatttatgat ccaggtggat actttgagaa agtgtaccaa actgctaaat
     3421 cggtagaagc ccaaaagttc cacgatgcga tctgtgccct tatcgtagaa gagctgtttg
     3481 aatatgcagg caaatggcgt aatattcgtg tgcaaggacc gacaacattt ctaccatcct
     3541 tgactgtaca ggtggcaatg gcaggtgcca tgttgattgg tctgcatcat cgcatctgtt
     3601 atacgacgag cgcttcggtc ttaactgaag cagttaagca accagatctt cctccaggtt
     3661 atgtccaact gtgccagctc gtaatgtctg gtcaactttc cgaccctgag aaacttctgg
     3721 aatcgctaga gaatttctgg aatggggttc aggagtgggc ggaacgacac ggatatatag
     3781 tggatgtgtc aaaacgcata ccattttgat gacattaaga aaggtggttt ttatgctcga
     3841 ggagctcggt accggatcct ctagatagta cctagattta gatgtctaaa aagcttttta
     3901 gacatctaat cttttctgaa gtacatccgc aactgtccat actcagaaca tgtgagcaaa
     3961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4441 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
