LOCUS       40924_6706        8219 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBlTAL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Anasontzis GE, Stathopoulou PM, Hatzinikolaou DG.
  TITLE     Homologous overexpression of transaldolase in Fusarium oxysporum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Anasontzis GE, Stathopoulou PM, Hatzinikolaou DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Laboratory of Microbiology, Sector of 
            Botany, Department of Biology, National and Kapodistrian University 
            of Athens, Zografou Campus, Zografou, Attiki 15784, Greece
REFERENCE   3  (bases 1 to 8219)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8219)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2011) Laboratory of Microbiology, Sector of Botany, 
            Department of Biology, National and Kapodistrian University of 
            Athens, Zografou Campus, Zografou, Attiki 15784, Greece"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        673..1029
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             1040..2062
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      2235..2793
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     primer_bind     3086..3102
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(3184..3740)
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     CDS             complement(join(3745..3978,4042..4425,4484..4767,4925..4936,
                     4996..5014,5237..5275))
                     /codon_start=1
                     /product="transaldolase"
                     /label=transaldolase
                     /note="from Fusarium oxysporum"
                     /protein_id="AEP71396.1"
                     /translation="MSSSLEQLKATGTTVVSDSGDFASIGKYKPQDATTNPSLILAASK
                     KAEYAKLIDVAIDYAKQKGGPIDQQVDDALDRLLVEFGKEILKIIPGKVSTEVDARYSF
                     DTEASVNKALHLIELYGEQGISKDRILIKIAATWEGIKAAEILQRDHGINTNLTLMFSL
                     VQAIGAAEAGAYLISPFVGRILDWFKASTKKEYSKEEDPGVQSVKTIFNYYKKYGYNTI
                     VMGASFRNTGEITELAGCDYLTISPNLLEELLNSNEPVPKKLDASQASSLDIEKKSYIN
                     DEALFRFDFNEDQMAVEKLREGISKFAADAVTLKSILKEKLA"
     regulatory      complement(5284..6004)
                     /label=gpdA promoter from Aspergillus nidulans
                     /note="gpdA promoter from Aspergillus nidulans"
                     /regulatory_class="promoter"
     promoter        complement(6031..6049)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6070..6086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6094..6110)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6118..6148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6163..6184)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6472..7060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7234..8091)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8092..8196)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacagaagat gatattgaag gagcattttt gggcttggct ggagctagtg
      721 gaggtcaaca catcaatgct attttggttt agtcgtccag gcggatcaca aaatttgtgt
      781 cgtttgacaa gatggttcat ttaggcaact ggtcagatca gcccacttgt aagcagtagc
      841 ggcggcgctc gaagtgtgac tcttattagc agacaggaac gaggacatta ttatcatctg
      901 ctgcttggtg cacgataact tgtgcgtttg tcaagcaagg taagtgaacg acccggtcat
      961 accttcttaa gttcgccctt cctcccttta tttcagattc aatctgactt acctattcta
     1021 ccgaagcatc gatatcgata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     1081 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     1141 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     1201 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     1261 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     1321 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     1381 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     1441 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     1501 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     1561 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     1621 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     1681 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     1741 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     1801 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     1861 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     1921 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     1981 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     2041 cactcgtccg agggcaaagg aatagagtag atgccgaccg ggatccactt aacgttactg
     2101 aaatcatcaa acagcttgac gaatctggat ataagatcgt tggtgtcgat gtcagctccg
     2161 gagttgagac aaatggtgtt caggatctcg ataagatacg ttcatttgtc caagcagcaa
     2221 agagtgcctt ctagtgattt aatagctcca tgtcaacaag aataaaacgc gttttcgggt
     2281 ttacctcttc cagatacagc tcatctgcaa tgcattaatg cattgactgc aacctagtaa
     2341 cgccttcagg ctccggcgaa gagaagaata gcttagcaga gctattttca ttttcgggag
     2401 acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg
     2461 gctccacgcg actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc
     2521 tgatagcttg actatgaaaa ttccgtcacc agccctgggt tcgcaaagat aattgcatgt
     2581 ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
     2641 tcattcctac taagatggta tacaatagta accatggttg cctagtgaat gctccgtaac
     2701 acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc
     2761 acaggtacac ttgtttagag gtaatccttc tttctagagg atcctctacg ccggacgcat
     2821 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     2881 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     2941 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     3001 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     3061 gcataaggga gggacgcatc agcgtcgagg tcgacggtat cgataagctt gatatcgaat
     3121 tcctgcagcc cgggcgagtg gagatgtgga gtgggcgctt acacagtaca cgaggacttc
     3181 tagaaagaag gattacctct aaacaagtgt acctgtgcat tctgggtaaa cgactcatag
     3241 gagagttgta aaaaagtttc ggccggcgta ttgggtgtta cggagcattc actaggcaac
     3301 catggttact attgtatacc catcttagta ggaatgattt tcgaggttta tacctacgat
     3361 gaatgtgtgt cctgtaggct tgagagttca aggaagaaac agtgcaatta tctttgcgaa
     3421 cccaggggct ggtgacggaa ttttcatagt caagctatca gagtaaagaa gaggagcatg
     3481 tcaaagtaca attagagaca aatatatagt cgcgtggagc caagagcgga ttcctcagtc
     3541 tcgtaggtct cttgacgacc gttgatctgc ttgatctcgt ctcccgaaaa tgaaaataga
     3601 ctctgctaag ctattcttct gcttcgccgg agcctgaagg gcgtactagg gttgcgaggt
     3661 ccaatgcatt aatgcattgc agatgagctg tatctggaag aggtaaaccc gaaacgcgtt
     3721 ttattcttgt tgacatggac tagtttaggc gagcttctcc ttgaggatgc tcttaagggt
     3781 gacagcatca gcagcgaact tgctgatacc ctctcggagc ttctcaacgg ccatctggtc
     3841 ctcgttgaag tcaaagcgga agagagcctc atcattgatg taggacttct tctcgatgtc
     3901 tagggaggaa gcctgggaag catcaagctt cttgggaacg ggctcattcg agttcaggag
     3961 ctcctcaagc aggttaggct gtagaaattg aaaattagca tacttgattc ctattcgcaa
     4021 cacaggtttg atgtgacata cagagatggt caagtagtcg cagccagcaa gctcagtgat
     4081 ctcgccagtg tttcggaaag aggcgcccat gacgatggtg ttgtaaccgt acttcttgta
     4141 gtagttgaag atggtcttga cggactgaac accagggtcc tcctccttag agtattcctt
     4201 cttggtagag gccttgaacc agtcaagaat gcggccgacg aagggagaga taaggtaggc
     4261 accggcctcg gcagcgccaa tagcttgaac aagagagaac atgagggtga ggttggtgtt
     4321 gatgccgtgg tcgcgctgga gaatctcagc agccttgatg ccctcccaag tggcggcgat
     4381 cttgatcaga atgcgatcct tggagatacc ctgttcgccg taaagcttta aatttgcgtt
     4441 agcacttcgt ctagagtgat gccctgctgc tatggtggct tacctcaatg aggtgaaggg
     4501 ccttgttgac ggaagcctcg gtatcgaaag agtatcgggc atcgacctca gttgagacct
     4561 tgccgggaat gatcttgagg atctccttgc caaactcgac aagaagacgg tcaagagcat
     4621 catcgacctg ctggtcgata gggccaccct tctgcttggc gtagtcaatg gcgacatcga
     4681 tcaacttggc gtactcggcc ttcttggagg cagcgagaat gagagaaggg ttggtggtgg
     4741 catcctgagg cttgtacttg ccaatggctg ttgaaatgtc agctgctgtt cattctctca
     4801 tatcatgcca tgtcacatca tggcgatcat gactccttat gtcatatggg cgaagttgga
     4861 actcctggtt aggatagcag gagtcgtggg ctcggggcgc ataatgatag gagtgtatac
     4921 taacaggcaa agtcgcctaa gagtaagaag ttagtaaaga agtacttgat cgagtccagg
     4981 aggtgcggta attaccagag tcagacacaa cagtctattg aggtgggagc aaaccaaatg
     5041 gttagccatc aatttctcat aagagacata gacatggaaa ggcgaaaaca acatctgatc
     5101 acgaggatga ccaagaacga tgaattggtg agtccaatta atcagaggtg ttgtttatca
     5161 agcttaaaca tgatgccatg ctcgcttttg ttcgcgttca caaacgagtt ggaggggtaa
     5221 tggtggggtg acatacagtg ccagtggcct tgagctgttc gagagaggaa gacatgcggc
     5281 cgcgggaaga tgaatatact gaagatggga aaagaaagag aaaagaaaag agcagctggt
     5341 ggggagagca ggaaaatatg gcaacaaatg ttggactgac gcaacgacct tgtcaacccc
     5401 gccgacacac cgggcggaca gacggggcaa agctgcctac cagggactga gggacctcag
     5461 caggtcgagt gcagagcacc ggatgggtcg actgccagct tgtgttcccg gtctgcgccg
     5521 ctggccagct cctgagcggc ctttccggtt tcatacaccg ggcaaagcag gagaggcacg
     5581 atatttggac gccctacaga tgccggatgg gccaattagg gagcttacgc gccgggtact
     5641 cgctctacct acttcggaga aggtactatc tcgtgaatct tttaccagat cggaagcaat
     5701 tggacttctg tacctaggtt aatggcatgc tatttcgccg acggctatac acccctggct
     5761 tcacattctc cttcgcttac tgccggtgat tcgatgaagc tccatattct ccgatgatgc
     5821 aatagattct tggtcaacga ggggcacacc agcctttcca cttcggggcg gaggggcggc
     5881 cggtcccgga ttaataatca tccactgcac ctcagagccg ccagagctgt ctggccagtg
     5941 gcttattact cagcccttct ctctgcgtcc gtccgtctct ccgcatgcca gaaagagtca
     6001 ccggccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     6061 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6901 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
