LOCUS       Exported               14112 bp DNA     circular SYN 21-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBin-35S-mCherry-MCS-NOS-Neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14112)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1115..1909
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             2208..3356
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPPETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4952..4976
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5449..5632
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5653..6447
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      6837..7089
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7638..7659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7674..7704
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7712..7728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7736..7752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8022..8729)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        complement(8775..9119)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9565..9581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9879..9903
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10368..11018
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     oriT            11699..11808
                     /note="incP origin of transfer"
     CDS             11841..12212
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      12339..13050
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
       61 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      121 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      181 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      241 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      301 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      421 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      481 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      541 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agttaccgac
      601 tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc ataatgcggg
      661 ctgttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg tgcgtaccgg
      721 gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt acggcagtga gagcagagat
      781 agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg
      841 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
      901 atcagtaagt tggcagcatc acccataatt gtggtttcaa aatcggctcc gtcgatacta
      961 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     1021 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     1081 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     1141 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     1201 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     1261 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     1321 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     1381 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     1441 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     1501 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     1561 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     1621 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     1681 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     1741 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     1801 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     1861 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagatgt
     1921 ggcgcaacga tgccggcgac aagcaggagc gcaccgactt cttccgcatc aagtgttttg
     1981 gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg gtcgctggta ttcgtgcagg
     2041 gcaagattcg gaataccaag tacgagaagg acggccagac ggtctacggg accgacttca
     2101 ttgccgataa ggtggattat ctggacacca aggcaccagg cgggtcaaat caggaataag
     2161 ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg agggtgaatg aatcggacgt
     2221 ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc gaggatgccg
     2281 aaaccatcgc aagccgcacc gtcatgcgtg cgccccccga aaccttccag tccgtcggct
     2341 cgatggtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg gctccccctg
     2401 ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tctcgaacag gaggcggcag
     2461 gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag aagcgaaaaa
     2521 ccgccggcga ggacctggca aaacaggtca gcgaggccaa gcaggccgcg ttgctgaaac
     2581 acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg ccgtggccgg
     2641 acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc acgcgcaaca
     2701 agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac aaggacgtga
     2761 agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg tggcagcagg
     2821 tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg ttctacgagc
     2881 tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc gaggaatgcc
     2941 tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg cacctggaat
     3001 cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg tcccgttgcc
     3061 aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac acgaaattca
     3121 tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac tatttcagct
     3181 cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc ggatcggatt
     3241 ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag ttgcgaggca
     3301 gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa cgctagggcc
     3361 ttgtggggtc agttccggct gggggttcag cagccagcgc tttactggca tttcaggaac
     3421 aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg ctcgggacgc acggcgcgct
     3481 ctacgaactg ccgataaaca gaggattaaa attgacaatt gtgattaagg ctcagattcg
     3541 acggcttgga gcggccgacg tgcaggattt ccgcgagatc cgattgtcgg ccctgaagaa
     3601 agctccagag atgttcgggt ccgtttacga gcacgaggag aaaaagccca tggaggcgtt
     3661 cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac atcgacggcg agatcattgg
     3721 gctgtcggtc ttcaaacagg aggacggccc caaggacgct cacaaggcgc atctgtccgg
     3781 cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc ggtatgctgc tgcgggcgtt
     3841 gccggcgggt ttattgctcg tgatgatcgt ccgacagatt ccaacgggaa tctggtggat
     3901 gcgcatcttc atcctcggcg cacttaatat ttcgctattc tggagcttgt tgtttatttc
     3961 ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc gctgtgcagc cgctgatggt
     4021 cgtgttcatc tctgccgctc tgctaggtag cccgatacga ttgatggcgg tcctgggggc
     4081 tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca ccaaacgcag cgctagatcc
     4141 tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg gcgttcggaa ccgtgctgac
     4201 ccgcaagtgg caacctcccg tgcctctgct cacctttacc gcctggcaac tggcggccgg
     4261 aggacttctg ctcgttccag tagctttagt gtttgatccg ccaatcccga tgcctacagg
     4321 aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga gcgggtttaa cctacttcct
     4381 ttggttccgg gggatctcgc gactcgaacc tacagttgtt tccttactgg gctttctcag
     4441 ccccagatct ggggtcgatc agccggggat gcatcaggcc gacagtcgga acttcgggtc
     4501 cccgacctgt accattcggt gagcaatgga taggggagtt gatatcgtca acgttcactt
     4561 ctaaagaaat agcgccactc agcttcctca gcggctttat ccagcgattt cctattatgt
     4621 cggcatagtt ctcaagatcg acagcctgtc acggttaagc gagaaatgaa taagaaggct
     4681 gataattcgg atctctgcga gggagatgat atttgatcac aggcagcaac gctctgtcat
     4741 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
     4801 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     4861 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     4921 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     4981 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggttttt
     5041 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     5101 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     5161 ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg
     5221 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     5281 aaaccgtcta tcagggcgat ggcccacaaa ctgaaggcgg gaaacgacaa tctgatcatg
     5341 agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
     5401 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg gtttctggag
     5461 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
     5521 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
     5581 cctcggtatc caattagagt ctcatattca ctctcaatcc aaataatctg caccggatct
     5641 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5701 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5761 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5821 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5881 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5941 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6001 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6061 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6121 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6181 cgcatgcccg acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6241 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6301 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6361 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6421 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     6481 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6541 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6601 tggagttctt cgcccacggg atctctgcgg aacaggcggt cgaaggtgcc gatatcatta
     6661 cgacagcaac ggccgacaag cacaacgcca cgatcctgag cgacaatatg atcgggcccg
     6721 gcgtccacat caacggcgtc ggcggcgact gcccaggcaa gaccgagatg caccgcgata
     6781 tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca gacccggatg atccccgatc
     6841 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     6901 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     6961 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     7021 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     7081 tactagatcg ggcctcctgt caatgctggc ggcggctctg gtggtggttc tggtggcggc
     7141 tctgagggtg gtggctctga gggtggcggt tctgagggtg gcggctctga gggaggcggt
     7201 tccggtggtg gctctggttc cggtgatttt gattatgaaa agatggcaaa cgctaataag
     7261 ggggctatga ccgaaaatgc cgatgaaaac gcgctacagt ctgacgctaa aggcaaactt
     7321 gattctgtcg ctactgatta cggtgctgct atcgatggtt tcattggtga cgtttccggc
     7381 cttgctaatg gtaatggtgc tactggtgat tttgctggct ctaattccca aatggctcaa
     7441 gtcggtgacg gtgataattc acctttaatg aataatttcc gtcaatattt accttccctc
     7501 cctcaatcgg ttgaatgtcg cccttttgtc tttggcccaa tacgcaaacc gcctctcccc
     7561 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7621 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7681 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7741 aacagctatg accatgatta cgccaagctg gcgcgccaag cttgtcactg gattttggtt
     7801 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
     7861 ttcttatatg ctcaacacat gagcgaaacc ctataagaac cctaattccc ttatctggga
     7921 actactcaca cattattctg gagaaaaata gagagagata gatttgtaga gagagactgg
     7981 tgatttttgc ggactctagt tcatctagag gatccgtcga ccttgtacag ctcgtccatg
     8041 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
     8101 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
     8161 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
     8221 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
     8281 gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
     8341 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
     8401 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
     8461 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
     8521 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
     8581 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
     8641 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
     8701 atgttatcct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag cggatctgac
     8761 ggttcactct cgaggtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     8821 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
     8881 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     8941 gtccatcttt gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc
     9001 aatgatggca tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga
     9061 tagctgggca atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa
     9121 ttgccctttg gtcttctgag actgtatctt tgatattttt ggagtagaca agtgtgtcgt
     9181 gctccaccat gttatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     9241 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     9301 cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt
     9361 ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg
     9421 atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga
     9481 tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt gacctgcagg catgcaagga
     9541 gctcgaattc ttaattaaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     9601 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     9661 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgcccg
     9721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9841 aacttgattt gggtgatggt tcacaaacta tcagtgtttg acaggatata ttggcgggta
     9901 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     9961 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga tctggcgccg
    10021 gccagcgaga cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca
    10081 ggtgcgcagg caaattgcac caacgcatac agcgccagca gaatgccata gtgggcggtg
    10141 acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg
    10201 atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtat gttgggtttc
    10261 acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact
    10321 gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc
    10381 agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc
    10441 tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact
    10501 tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata
    10561 cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca
    10621 gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac
    10681 cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt
    10741 tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg
    10801 tgcttgagga gcaggccggc gacagcgatg ccggcgagcg cggcggcacc gttgaacagg
    10861 ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg
    10921 cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg
    10981 tcaggaacgt tgaaggaccg agaaagggtg acgattgatc aggaccgctg ccggagcgca
    11041 acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc accgcgtcag
    11101 acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc aagcctcacg
    11161 gccgcgctcg gcctctctgg cggccttctg gcgctcttcc gcttcctcgc tcactgactc
    11221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgcttttcc gctgcataac
    11581 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
    11641 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
    11701 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
    11761 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg
    11821 taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac
    11881 ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg
    11941 ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg
    12001 tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg
    12061 gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca
    12121 cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc aaggtcatga
    12181 tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa cggccggggg
    12241 gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga cttcgcggag
    12301 ctggtgaagt acatcaccga cgagcaaggc aagaccgagc gcctttccga cgctcaccgg
    12361 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
    12421 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
    12481 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
    12541 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
    12601 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag
    12661 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
    12721 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac
    12781 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
    12841 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
    12901 taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc
    12961 tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg
    13021 ccgcgaacgg cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg
    13081 gggcagtaac gggatgggcg atcagcccga gcgcgacgcc cggaagcatt gacgtgccgc
    13141 aggtgctggc atcgacattc agcgaccagg tgccgggcag tgagggcggc ggcctgggtg
    13201 gcggcctgcc cttcacttcg gccgtcgggg cattcacgga cttcatggcg gggccggcaa
    13261 tttttacctt gggcattctt ggcatagtgg tcgcgggtgc cgtgctcgtg ttcgggggtg
    13321 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
    13381 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
    13441 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
    13501 aatatatctt ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
    13561 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
    13621 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgggtaatga
    13681 ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct
    13741 ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat
    13801 tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct
    13861 tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg
    13921 acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa
    13981 caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc
    14041 cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta
    14101 tgcgcgaggt ta
//
