LOCUS pBiT2.1-TOP1(human) 5676 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029734 VERSION V029734 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 28..779 /note="herpes simplex virus thymidine kinase promoter" /label="HSV TK promoter" regulatory 809..818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 893..3187 /label="TOP1(NM_003286)" /note="TOP1(NM_003286)" /gene="TOP1" polyA_signal 3230..3351 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3770..4358) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4558..5418) /codon_start=1 /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" polyA_signal 5523..5571 /note="synthetic polyadenylation signal" /label="poly(A) signal" misc_feature 5585..5676 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /label="pause site" ORIGIN 1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa 781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga 841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgagtgg 901 ggaccacctc cacaacgatt cccagatcga agcggatttc cgattgaatg attctcataa 961 acacaaagat aaacacaaag atcgagaaca ccggcacaaa gaacacaaga aggagaagga 1021 ccgggaaaag tccaagcata gcaacagtga acataaagat tctgaaaaga aacacaaaga 1081 gaaggagaag accaaacaca aagatggaag ctcagaaaag cataaagaca aacataaaga 1141 cagagacaag gaaaaacgaa aagaggaaaa ggttcgagcc tctggggatg caaaaataaa 1201 gaaggagaag gaaaatggct tctctagtcc accacaaatt aaagatgaac ctgaagatga 1261 tggctatttt gttcctccta aagaggatat aaagccatta aagagacctc gagatgagga 1321 tgatgctgat tataaaccta agaaaattaa aacagaagat accaagaagg agaagaaaag 1381 aaaactagaa gaagaagagg atggtaaatt gaaaaaaccc aagaataaag ataaagataa 1441 aaaagttcct gagccagata acaagaaaaa gaagccgaag aaagaagagg aacagaagtg 1501 gaaatggtgg gaagaagagc gctatcctga aggcatcaag tggaaattcc tagaacataa 1561 aggtccagta tttgccccac catatgagcc tcttccagag aatgtcaagt tttattatga 1621 tggtaaagtc atgaagctga gccccaaagc agaggaagta gctacgttct ttgcaaaaat 1681 gctcgaccat gaatatacta ccaaggaaat atttaggaaa aatttcttta aagactggag 1741 aaaggaaatg actaatgaag agaagaatat tatcaccaac ctaagcaaat gtgattttac 1801 ccagatgagc cagtatttca aagcccagac ggaagctcgg aaacagatga gcaaggaaga 1861 gaaactgaaa atcaaagagg agaatgaaaa attactgaaa gaatatggat tctgtattat 1921 ggataaccac aaagagagga ttgctaactt caagatagag cctcctggac ttttccgtgg 1981 ccgcggcaac caccccaaga tgggcatgct gaagagacga atcatgcccg aggatataat 2041 catcaactgt agcaaagatg ccaaggttcc ttctcctcct ccaggacata agtggaaaga 2101 agtccggcat gataacaagg ttacttggct ggtttcctgg acagagaaca tccaaggttc 2161 cattaaatac atcatgctta accctagttc acgaatcaag ggtgagaagg actggcagaa 2221 atacgagact gctcggcggc tgaaaaaatg tgtggacaag atccggaacc agtatcgaga 2281 agactggaag tccaaagaga tgaaagtccg gcagagagct gtagccctgt acttcatcga 2341 caagcttgct ctgagagcag gcaatgaaaa ggaggaagga gaaacagcgg acactgtggg 2401 ctgctgctca cttcgtgtgg agcacatcaa tctacaccca gagttggatg gtcaggaata 2461 tgtggtagag tttgacttcc tcgggaagga ctccatcaga tactataaca aggtccctgt 2521 tgagaaacga gtttttaaga acctacaact atttatggag aacaagcagc ccgaggatga 2581 tctttttgat agactcaata ctggtattct gaataagcat cttcaggatc tcatggaggg 2641 cttgacagcc aaggtattcc gtacatacaa tgcctccatc acgctacagc agcagctaaa 2701 agaactgaca gccccggatg agaacatccc agcgaagatc ctttcttata accgtgccaa 2761 tcgagctgtt gcaattcttt gtaaccatca gagggcacca ccaaaaactt ttgagaagtc 2821 tatgatgaac ttgcaaacta agattgatgc caagaaggaa cagctagcag atgcccggag 2881 agacctgaaa agtgctaagg ctgatgccaa ggtcatgaag gatgcaaaga cgaagaaggt 2941 agtagagtca aagaagaagg ctgttcagag actggaggaa cagttgatga agctggaagt 3001 tcaagccaca gaccgagagg aaaataaaca gattgccctg ggaacctcca aactcaatta 3061 tctggaccct aggatcacag tggcttggtg caagaagtgg ggtgtcccaa ttgagaagat 3121 ttacaacaaa acccagcggg agaagtttgc ctgggccatt gacatggctg atgaagacta 3181 tgagttttag tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt 3241 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 3301 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 3361 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag 3421 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc 3481 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 3541 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct 3601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4501 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta 4561 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt 4621 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag 4681 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca 4741 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc 4801 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt 4861 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa 4921 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt 4981 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat 5041 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt 5101 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc 5161 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat 5221 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag 5281 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt 5341 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg 5401 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta 5461 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc 5521 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag 5581 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg 5641 tccccagtgc aagtgcaggt gccagaacat ttctct //