LOCUS       pBiT2.1-TOP1(human)     5676 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029734
VERSION     V029734
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..3187
                     /label="TOP1(NM_003286)"
                     /note="TOP1(NM_003286)"
                     /gene="TOP1"
     polyA_signal    3230..3351
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3770..4358)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4558..5418)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    5523..5571
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    5585..5676
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgagtgg
      901 ggaccacctc cacaacgatt cccagatcga agcggatttc cgattgaatg attctcataa
      961 acacaaagat aaacacaaag atcgagaaca ccggcacaaa gaacacaaga aggagaagga
     1021 ccgggaaaag tccaagcata gcaacagtga acataaagat tctgaaaaga aacacaaaga
     1081 gaaggagaag accaaacaca aagatggaag ctcagaaaag cataaagaca aacataaaga
     1141 cagagacaag gaaaaacgaa aagaggaaaa ggttcgagcc tctggggatg caaaaataaa
     1201 gaaggagaag gaaaatggct tctctagtcc accacaaatt aaagatgaac ctgaagatga
     1261 tggctatttt gttcctccta aagaggatat aaagccatta aagagacctc gagatgagga
     1321 tgatgctgat tataaaccta agaaaattaa aacagaagat accaagaagg agaagaaaag
     1381 aaaactagaa gaagaagagg atggtaaatt gaaaaaaccc aagaataaag ataaagataa
     1441 aaaagttcct gagccagata acaagaaaaa gaagccgaag aaagaagagg aacagaagtg
     1501 gaaatggtgg gaagaagagc gctatcctga aggcatcaag tggaaattcc tagaacataa
     1561 aggtccagta tttgccccac catatgagcc tcttccagag aatgtcaagt tttattatga
     1621 tggtaaagtc atgaagctga gccccaaagc agaggaagta gctacgttct ttgcaaaaat
     1681 gctcgaccat gaatatacta ccaaggaaat atttaggaaa aatttcttta aagactggag
     1741 aaaggaaatg actaatgaag agaagaatat tatcaccaac ctaagcaaat gtgattttac
     1801 ccagatgagc cagtatttca aagcccagac ggaagctcgg aaacagatga gcaaggaaga
     1861 gaaactgaaa atcaaagagg agaatgaaaa attactgaaa gaatatggat tctgtattat
     1921 ggataaccac aaagagagga ttgctaactt caagatagag cctcctggac ttttccgtgg
     1981 ccgcggcaac caccccaaga tgggcatgct gaagagacga atcatgcccg aggatataat
     2041 catcaactgt agcaaagatg ccaaggttcc ttctcctcct ccaggacata agtggaaaga
     2101 agtccggcat gataacaagg ttacttggct ggtttcctgg acagagaaca tccaaggttc
     2161 cattaaatac atcatgctta accctagttc acgaatcaag ggtgagaagg actggcagaa
     2221 atacgagact gctcggcggc tgaaaaaatg tgtggacaag atccggaacc agtatcgaga
     2281 agactggaag tccaaagaga tgaaagtccg gcagagagct gtagccctgt acttcatcga
     2341 caagcttgct ctgagagcag gcaatgaaaa ggaggaagga gaaacagcgg acactgtggg
     2401 ctgctgctca cttcgtgtgg agcacatcaa tctacaccca gagttggatg gtcaggaata
     2461 tgtggtagag tttgacttcc tcgggaagga ctccatcaga tactataaca aggtccctgt
     2521 tgagaaacga gtttttaaga acctacaact atttatggag aacaagcagc ccgaggatga
     2581 tctttttgat agactcaata ctggtattct gaataagcat cttcaggatc tcatggaggg
     2641 cttgacagcc aaggtattcc gtacatacaa tgcctccatc acgctacagc agcagctaaa
     2701 agaactgaca gccccggatg agaacatccc agcgaagatc ctttcttata accgtgccaa
     2761 tcgagctgtt gcaattcttt gtaaccatca gagggcacca ccaaaaactt ttgagaagtc
     2821 tatgatgaac ttgcaaacta agattgatgc caagaaggaa cagctagcag atgcccggag
     2881 agacctgaaa agtgctaagg ctgatgccaa ggtcatgaag gatgcaaaga cgaagaaggt
     2941 agtagagtca aagaagaagg ctgttcagag actggaggaa cagttgatga agctggaagt
     3001 tcaagccaca gaccgagagg aaaataaaca gattgccctg ggaacctcca aactcaatta
     3061 tctggaccct aggatcacag tggcttggtg caagaagtgg ggtgtcccaa ttgagaagat
     3121 ttacaacaaa acccagcggg agaagtttgc ctgggccatt gacatggctg atgaagacta
     3181 tgagttttag tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     3241 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3301 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3361 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     3421 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc
     3481 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3541 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     3601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4501 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     4561 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     4621 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     4681 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     4741 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     4801 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     4861 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     4921 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4981 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     5041 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     5101 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     5161 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     5221 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     5281 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     5341 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     5401 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     5461 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     5521 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     5581 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     5641 tccccagtgc aagtgcaggt gccagaacat ttctct
//
