LOCUS       pBiT2.1-PLOD2(human)    5704 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045632
VERSION     V045632
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     CDS             863..3136
                     /label="PLOD2(NM_182943)"
                     /note="PLOD2(NM_182943)"
                     /gene="PLOD2"
     polyA_signal    3258..3379
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3798..4386)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4586..5446)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    5551..5599
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    5613..5704
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg tggtaaagcc accagatctg ctagcgatcg cctaagtggg
      841 agctcagggg aattccgcca ccatgggggg atgcacggtg aagcctcagc tgctgctcct
      901 ggcgctcgtc ctccacccct ggaatccctg tctgggtgcg gactcggaga agccctcgag
      961 catccccaca gataaattat tagtcataac tgtagcaaca aaagaaagtg atggattcca
     1021 tcgatttatg cagtcagcca aatatttcaa ttatactgtg aaggtccttg gtcaaggaga
     1081 agaatggaga ggtggtgatg gaattaatag tattggaggg ggccagaaag tgagattaat
     1141 gaaagaagtc atggaacact atgctgatca agatgatctg gttgtcatgt ttactgaatg
     1201 ctttgatgtc atatttgctg gtggtccaga agaagttcta aaaaaattcc aaaaggcaaa
     1261 ccacaaagtg gtctttgcag cagatggaat tttgtggcca gataaaagac tagcagacaa
     1321 gtatcctgtt gtgcacattg ggaaacgcta tctgaattca ggaggattta ttggctatgc
     1381 tccatatgtc aaccgtatag ttcaacaatg gaatctccag gataatgatg atgatcagct
     1441 cttttacact aaagtttaca ttgatccact gaaaagggaa gctattaaca tcacattgga
     1501 tcacaaatgc aaaattttcc agaccttaaa tggagctgta gatgaagttg ttttaaaatt
     1561 tgaaaatggc aaagccagag ctaagaatac attttatgaa acattaccag tggcaattaa
     1621 tggaaatgga cccaccaaga ttctcctgaa ttattttgga aactatgtac ccaattcatg
     1681 gacacaggat aatggctgca ctctttgtga attcgataca gtcgacttgt ctgcagtaga
     1741 tgtccatcca aacgtatcaa taggtgtttt tattgagcaa ccaacccctt ttctacctcg
     1801 gtttctggac atattgttga cactggatta cccaaaagaa gcacttaaac tttttattca
     1861 taacaaagaa gtttatcatg aaaaggacat caaggtattt tttgataaag ctaagcatga
     1921 aatcaaaact ataaaaatag taggaccaga agaaaatcta agtcaagcgg aagccagaaa
     1981 catgggaatg gacttttgcc gtcaggatga aaagtgtgat tattacttta gtgtggatgc
     2041 agatgttgtt ttgacaaatc caaggacttt aaaaattttg attgaacaaa acagaaagat
     2101 cattgctcct cttgtaactc gtcatggaaa gctgtggtcc aatttctggg gagcattgag
     2161 tcctgatgga tactatgcac gatctgaaga ttatgtggat attgttcaag ggaatagagt
     2221 aggagtatgg aatgtcccat atatggctaa tgtgtactta attaaaggaa agacactccg
     2281 atcagagatg aatgaaagga actattttgt tcgtgataaa ctggatcctg atatggctct
     2341 ttgccgaaat gctagagaaa tgactttaca aagggaaaaa gactccccta ctccggaaac
     2401 attccaaatg ctcagccccc caaagggtgt atttatgtac atttctaata gacatgaatt
     2461 tggaaggcta ttatccactg ctaattacaa tacttcccat tataacaatg acctctggca
     2521 gatttttgaa aatcctgtgg actggaagga aaagtatata aaccgtgatt attcaaagat
     2581 tttcactgaa aatatagttg aacagccctg tccagatgtc ttttggttcc ccatattttc
     2641 tgaaaaagcc tgtgatgaat tggtagaaga aatggaacat tacggcaaat ggtctggggg
     2701 aaaacatcat gatagccgta tatctggtgg ttatgaaaat gtcccaactg atgatatcca
     2761 catgaagcaa gttgatctgg agaatgtatg gcttcatttt atccgggagt tcattgcacc
     2821 agttacactg aaggtctttg caggctatta tacgaaggga tttgcactac tgaattttgt
     2881 agtaaaatac tcccctgaac gacagcgttc tcttcgtcct catcatgatg cttctacatt
     2941 taccataaac attgcactta ataacgtggg agaagacttt cagggaggtg gttgcaaatt
     3001 tctaaggtac aattgctcta ttgagtcacc acgaaaaggc tggagcttca tgcatcctgg
     3061 gagactcaca catttgcatg aaggacttcc tgttaaaaat ggaacaagat acattgcagt
     3121 gtcatttata gatccctagc tcgagcggtg gtggcgggag cggaggtgga gggtcgtcag
     3181 gtgtgaccgg ctaccggctg ttcgaggaga ttctgtaatc tagagtcggg gcggccggcc
     3241 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3301 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3361 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     3481 ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     3541 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     3601 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     4561 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     4621 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     4681 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     4741 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     4801 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     4861 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     4921 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     4981 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     5041 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     5101 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     5161 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     5221 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     5281 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     5341 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     5401 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     5461 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
     5521 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
     5581 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     5641 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     5701 ctct
//
