LOCUS pBiT2.1-PLOD2(human) 5704 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045632 VERSION V045632 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 28..779 /note="herpes simplex virus thymidine kinase promoter" /label="HSV TK promoter" CDS 863..3136 /label="PLOD2(NM_182943)" /note="PLOD2(NM_182943)" /gene="PLOD2" polyA_signal 3258..3379 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3798..4386) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4586..5446) /codon_start=1 /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" polyA_signal 5551..5599 /note="synthetic polyadenylation signal" /label="poly(A) signal" misc_feature 5613..5704 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /label="pause site" ORIGIN 1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa 781 agcttggcaa tccggtactg tggtaaagcc accagatctg ctagcgatcg cctaagtggg 841 agctcagggg aattccgcca ccatgggggg atgcacggtg aagcctcagc tgctgctcct 901 ggcgctcgtc ctccacccct ggaatccctg tctgggtgcg gactcggaga agccctcgag 961 catccccaca gataaattat tagtcataac tgtagcaaca aaagaaagtg atggattcca 1021 tcgatttatg cagtcagcca aatatttcaa ttatactgtg aaggtccttg gtcaaggaga 1081 agaatggaga ggtggtgatg gaattaatag tattggaggg ggccagaaag tgagattaat 1141 gaaagaagtc atggaacact atgctgatca agatgatctg gttgtcatgt ttactgaatg 1201 ctttgatgtc atatttgctg gtggtccaga agaagttcta aaaaaattcc aaaaggcaaa 1261 ccacaaagtg gtctttgcag cagatggaat tttgtggcca gataaaagac tagcagacaa 1321 gtatcctgtt gtgcacattg ggaaacgcta tctgaattca ggaggattta ttggctatgc 1381 tccatatgtc aaccgtatag ttcaacaatg gaatctccag gataatgatg atgatcagct 1441 cttttacact aaagtttaca ttgatccact gaaaagggaa gctattaaca tcacattgga 1501 tcacaaatgc aaaattttcc agaccttaaa tggagctgta gatgaagttg ttttaaaatt 1561 tgaaaatggc aaagccagag ctaagaatac attttatgaa acattaccag tggcaattaa 1621 tggaaatgga cccaccaaga ttctcctgaa ttattttgga aactatgtac ccaattcatg 1681 gacacaggat aatggctgca ctctttgtga attcgataca gtcgacttgt ctgcagtaga 1741 tgtccatcca aacgtatcaa taggtgtttt tattgagcaa ccaacccctt ttctacctcg 1801 gtttctggac atattgttga cactggatta cccaaaagaa gcacttaaac tttttattca 1861 taacaaagaa gtttatcatg aaaaggacat caaggtattt tttgataaag ctaagcatga 1921 aatcaaaact ataaaaatag taggaccaga agaaaatcta agtcaagcgg aagccagaaa 1981 catgggaatg gacttttgcc gtcaggatga aaagtgtgat tattacttta gtgtggatgc 2041 agatgttgtt ttgacaaatc caaggacttt aaaaattttg attgaacaaa acagaaagat 2101 cattgctcct cttgtaactc gtcatggaaa gctgtggtcc aatttctggg gagcattgag 2161 tcctgatgga tactatgcac gatctgaaga ttatgtggat attgttcaag ggaatagagt 2221 aggagtatgg aatgtcccat atatggctaa tgtgtactta attaaaggaa agacactccg 2281 atcagagatg aatgaaagga actattttgt tcgtgataaa ctggatcctg atatggctct 2341 ttgccgaaat gctagagaaa tgactttaca aagggaaaaa gactccccta ctccggaaac 2401 attccaaatg ctcagccccc caaagggtgt atttatgtac atttctaata gacatgaatt 2461 tggaaggcta ttatccactg ctaattacaa tacttcccat tataacaatg acctctggca 2521 gatttttgaa aatcctgtgg actggaagga aaagtatata aaccgtgatt attcaaagat 2581 tttcactgaa aatatagttg aacagccctg tccagatgtc ttttggttcc ccatattttc 2641 tgaaaaagcc tgtgatgaat tggtagaaga aatggaacat tacggcaaat ggtctggggg 2701 aaaacatcat gatagccgta tatctggtgg ttatgaaaat gtcccaactg atgatatcca 2761 catgaagcaa gttgatctgg agaatgtatg gcttcatttt atccgggagt tcattgcacc 2821 agttacactg aaggtctttg caggctatta tacgaaggga tttgcactac tgaattttgt 2881 agtaaaatac tcccctgaac gacagcgttc tcttcgtcct catcatgatg cttctacatt 2941 taccataaac attgcactta ataacgtggg agaagacttt cagggaggtg gttgcaaatt 3001 tctaaggtac aattgctcta ttgagtcacc acgaaaaggc tggagcttca tgcatcctgg 3061 gagactcaca catttgcatg aaggacttcc tgttaaaaat ggaacaagat acattgcagt 3121 gtcatttata gatccctagc tcgagcggtg gtggcgggag cggaggtgga gggtcgtcag 3181 gtgtgaccgg ctaccggctg ttcgaggaga ttctgtaatc tagagtcggg gcggccggcc 3241 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 3301 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 3361 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 3421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa 3481 ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 3541 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 3601 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 3661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 3721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 3781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 3841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 3901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 3961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 4021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 4081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 4141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 4201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 4261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 4321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 4441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 4501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc 4561 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag 4621 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga 4681 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac 4741 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc 4801 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa 4861 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac 4921 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat 4981 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa 5041 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg 5101 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg 5161 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc 5221 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact 5281 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat 5341 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg 5401 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc 5461 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata 5521 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg 5581 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg 5641 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt 5701 ctct //