LOCUS       pBiT2.1-EHMT2(human)    7102 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046024
VERSION     V046024
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             890..4588
                     /label="EHMT2(NM_001289413)"
                     /note="EHMT2(NM_001289413)"
                     /gene="EHMT2"
     polyA_signal    4656..4777
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5196..5784)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5984..6844)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    6949..6997
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    7011..7102
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga tgcggggtct
      901 accgagaggg agggggttga tgcgggcccg ggggaggggt cgtgcggccc ctccgggcag
      961 ccgaggccgc ggaagggggg ggccccacag aggaagaggt aggccccgga gcctactctc
     1021 tcttcccagg gcccaggcat cctggacccc ccaactctct actgggctga ccagccctcc
     1081 tgtcccttgt ctcccctccc agggggaggc ccccgctgag atgggggcgc tgctgctgga
     1141 gaaggaaacc agaggagcca ccgagagagt tcatggctct ttgggggaca cccctcgtag
     1201 tgaagaaacc ctgcccaagg ccacccccga ctccctggag cctgctggcc cctcatctcc
     1261 agcctctgtc actgtcactg ttggtgatga gggggctgac acccctgtag gggctacacc
     1321 actcattggg gatgaatctg agaatcttga gggagatggg gacctccgtg ggggccggat
     1381 cctgctgggc catgccacaa agtcattccc ctcttccccc agcaaggggg gttcctgtcc
     1441 tagccgggcc aagatgtcaa tgacaggggc gggaaaatca cctccatctg tccagagttt
     1501 ggctatgagg ctactgagta tgccaggagc ccagggagct gcagcagcag ggtctgaacc
     1561 ccctccagcc accacgagcc cagagggaca gcccaaggtc caccgagccc gcaaaaccat
     1621 gtccaaacca ggaaatggac agcccccggt ccctgagaag cggccccctg aaatacagca
     1681 tttccgcatg agtgatgatg tccactcact gggaaaggtg acctcagatc tggccaaaag
     1741 gaggaagctg aactcaggag gtggcctgtc agaggagtta ggttctgccc ggcgttcagg
     1801 agaagtgacc ctgacgaaag gggaccccgg gtccctggag gagtgggaga cggtggtggg
     1861 tgatgacttc agtctctact atgattccta ctctgtggat gagcgcgtgg actccgacag
     1921 caagtctgaa gttgaagctc taactgaaca actaagtgaa gaggaggagg aggaagagga
     1981 ggaagaagaa gaagaggaag aggaggagga agaggaagaa gaagaggaag atgaggagtc
     2041 agggaatcag tcagatagga gtggttccag tggccggcgc aaggccaaga agaaatggcg
     2101 aaaagacagc ccatgggtga agccgtctcg gaaacggcgc aagcgggagc ctccgcgggc
     2161 caaggagcca cgaggggtgt ccaatgacac atcttcgctg gagacagagc gagggtttga
     2221 ggagttgccc ctgtgcagct gccgcatgga ggcacccaag attgaccgca tcagcgagag
     2281 ggcggggcac aagtgcatgg ccactgagag tgtggacgga gagctgtcag gctgcaatgc
     2341 cgccatcctc aagcgggaga ccatgaggcc atccagccgt gtggccctga tggtgctctg
     2401 tgagacccac cgcgcccgca tggtcaaaca ccactgctgc ccgggctgcg gctacttctg
     2461 cacggcgggc accttcctgg agtgccaccc tgacttccgt gtggcccacc gcttccacaa
     2521 ggcctgtgtg tctcagctga atgggatggt cttctgtccc cactgtgggg aggatgcttc
     2581 tgaagctcaa gaggtgacca tcccccgggg tgacggggtg accccaccgg ccggcactgc
     2641 agctcctgca cccccacccc tgtcccagga tgtccccggg agagcagaca cttctcagcc
     2701 cagtgcccgg atgcgagggc atggggaacc ccggcgcccg ccctgcgatc ccctggctga
     2761 caccattgac agctcagggc cctccctgac cctgcccaat gggggctgcc tttcagccgt
     2821 ggggctgcca ctggggccag gccgggaggc cctggaaaag gccctggtca tccaggagtc
     2881 agagaggcgg aagaagctcc gtttccaccc tcggcagttg tacctgtccg tgaagcaggg
     2941 cgagctgcag aaggtgatcc tgatgctgtt ggacaacctg gaccccaact tccagagcga
     3001 ccagcagagc aagcgcacgc ccctgcatgc agccgcccag aagggctccg tggagatctg
     3061 ccatgtgctg ctgcaggctg gagccaacat aaatgcagtg gacaaacagc agcggacgcc
     3121 actgatggag gccgtggtga acaaccacct ggaggtagcc cgttacatgg tgcagcgtgg
     3181 tggctgtgtc tatagcaagg aggaggacgg ttccacctgc ctccaccacg cagccaaaat
     3241 cgggaacttg gagatggtca gcctgctgct gagcacagga caggtggacg tcaacgccca
     3301 ggacagtggg gggtggacgc ccatcatctg ggctgcagag cacaagcaca tcgaggtgat
     3361 ccgcatgcta ctgacgcggg gcgccgacgt caccctcact gacaacgagg agaacatctg
     3421 cctgcactgg gcctccttca cgggcagcgc cgccatcgcc gaagtccttc tgaatgcgcg
     3481 ctgtgacctc catgctgtca actaccatgg ggacaccccc ctgcacatcg cagctcggga
     3541 gagctaccat gactgcgtgc tgttattcct gtcacgtggg gccaaccctg agctgcggaa
     3601 caaagagggg gacacagcat gggacctgac tcccgagcgc tccgacgtgt ggtttgcgct
     3661 tcaactcaac cgcaagctcc gacttggggt gggaaatcgg gccatccgca cagagaagat
     3721 catctgccgg gacgtggctc ggggctatga gaacgtgccc attccctgtg tcaacggtgt
     3781 ggatggggag ccctgccctg aggattacaa gtacatctca gagaactgcg agacgtccac
     3841 catgaacatc gatcgcaaca tcacccacct gcagcactgc acgtgtgtgg acgactgctc
     3901 tagctccaac tgcctgtgcg gccagctcag catccggtgc tggtatgaca aggatgggcg
     3961 attgctccag gaatttaaca agattgagcc tccgctgatt ttcgagtgta accaggcgtg
     4021 ctcatgctgg agaaactgca agaaccgggt cgtacagagt ggcatcaagg tgcggctaca
     4081 gctctaccga acagccaaga tgggctgggg ggtccgcgcc ctgcagacca tcccacaggg
     4141 gaccttcatc tgcgagtatg tcggggagct gatctctgat gctgaggctg atgtgagaga
     4201 ggatgattct tacctcttcg acttagacaa caaggatgga gaggtgtact gcatagatgc
     4261 ccgttactat ggcaacatca gccgcttcat caaccacctg tgtgacccca acatcattcc
     4321 cgtccgggtc ttcatgctgc accaagacct gcgatttcca cgcatcgcct tcttcagttc
     4381 ccgagacatc cggactgggg aggagctagg gtttgactat ggcgaccgct tctgggacat
     4441 caaaagcaaa tatttcacct gccaatgtgg ctctgagaag tgcaagcact cagccgaagc
     4501 cattgccctg gagcagagcc gtctggcccg cctggaccca caccctgagc tgctgcccga
     4561 gctcggctcc ctgccccctg tcaacacatg agaattcagt ctaagctagc agatcttcta
     4621 gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     4681 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     4741 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4801 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     4861 atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca
     4921 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4981 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     5041 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5101 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5701 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5761 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5821 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5881 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5941 acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca
     6001 gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg
     6061 tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc
     6121 cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg
     6181 ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc
     6241 gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta
     6301 ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc
     6361 ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc
     6421 ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac
     6481 tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact
     6541 caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta
     6601 tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt
     6661 cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca
     6721 ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa
     6781 aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc
     6841 tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc
     6901 aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct
     6961 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     7021 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     7081 gcaggtgcca gaacatttct ct
//
