LOCUS pBiT2.1-DHX33(human) 5502 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029778 VERSION V029778 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 28..779 /note="herpes simplex virus thymidine kinase promoter" /label="HSV TK promoter" regulatory 809..818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 893..3013 /label="DHX33(NM_020162)" /note="DHX33(NM_020162)" /gene="DHX33" polyA_signal 3056..3177 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3596..4184) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4384..5244) /codon_start=1 /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" polyA_signal 5349..5397 /note="synthetic polyadenylation signal" /label="poly(A) signal" misc_feature 5411..5502 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /label="pause site" ORIGIN 1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa 781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga 841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgccgga 901 ggaggcgggc ttcccgccgg ccaagagatt ccggccaggc tctggacctc cgagccgcgc 961 tgggtccttc cctcccggga ggcaagtggt gatgctgctg actgcgggca gcggcggcag 1021 aggaggagga ggaggccgga ggcagcagcc gcccctggcc cagccctcgg ccagtcccta 1081 ccctgaagct gtggagctgc agcgccggag tctgcccatc ttccaagcgc gggggcaact 1141 gttggcccag ctccgaaacc tggacaacgc ggtcctcatc ggggaaactg gctctgggaa 1201 gacaactcag atccctcagt acctgtatga aggagggatc agccgccagg gcatcattgc 1261 tgtgacccag cctcgtcgag tagctgccat ctctcttgct actagagtct cagatgagaa 1321 gagaactgaa cttgggaagc tggttggcta tacagtgcgc tttgatgatg tcacctcaga 1381 agacaccagg atcaagtttc tgacagatgg catgcttctg cgtgaagcaa tttcagactc 1441 tttgcttcgg aaatacagct gtgtcatttt ggatgaagct cacgaacgga ctatccacac 1501 agatgtgctc tttggagtgg tgaaagctgc acagaagagg agaaaggaac tcgggaaact 1561 tcctctgaaa gtgattgtga tgtcagctac gatggatgtg gacctgttct ctcagtattt 1621 caatggcgcc cccgtcctct acctagaggg tcggcagcat ccgatccagg tgttttacac 1681 caaacagcct cagaatgatt acctgcacgc cgcgcttgtc tccgtcttcc agatccacca 1741 ggaagcccct tcttcacagg acatcctggt gttcctcact gggcaggagg agatcgaagc 1801 catgagcaag acgtgccgag acattgcaaa gcacctccca gacggctgcc ctgcgatgct 1861 ggtccttcct ctgtacgcct ccctgcccta tgcacagcag ctccgagtct tccaaggggc 1921 cccaaagggc tatcgcaaag tgatcatttc aaccaacatc gctgaaacct ccataaccat 1981 tacaggaata aaatatgtag ttgacacggg catggttaaa gcaaagaagt ataaccctga 2041 cagtggtctt gaggtgttag cagtgcagcg ggtatcgaag acgcaggctt ggcagcgcac 2101 agggagggct ggcagagagg acagtggcat ctgctaccgg ctctacacgg aggacgagtt 2161 tgagaagttt gataagatga ccgtgccaga gatccagagg tgtaacctgg ccagtgtgat 2221 gcttcagctt ctagcaatga aagtcccaaa tgtgctcacc tttgacttca tgtcgaagcc 2281 atctccagat cacattcagg cggccattgc ccaactggac ctgttaggtg ctcttgaaca 2341 taaggatgac cagcttaccc tgactccaat gggaagaaag atggcagcat ttcctttaga 2401 acccaaattt gccaaaacca tcctcatgtc ccccaaattc cactgtacag aggagatcct 2461 gaccattgtc tccctgctgt ctgtggacag cgtcctccac aaccctcctt cccggcgaga 2521 ggaagtgcaa ggggtccgca agaagttcat atccagcgag ggggatcaca tgaccctgct 2581 caatatctat cggaccttca aaaacctagg cggaaataag gattggtgca aagagaattt 2641 tgtcaacagc aagaatatga cgctggtagc agaagtcaga gcacagctga gggacatctg 2701 cttaaagatg tcaatgccaa tcgcatcatc ccgaggagac gtggagagtg tccgccgctg 2761 cctggctcac agcctcttca tgagcaccgc cgagcttcag ccagatggca cctatgccac 2821 cacggacacc caccagccag tggccatcca cccgtcgtct gtcctcttcc actgcaagcc 2881 ggcctgcgtc gtgtacactg agctgctcta caccaacaag tgctacatgc gggacctctg 2941 cgtcatagat gcacagtggc tgtacgaggc tgcccctgag tactttagga ggaagctgag 3001 aaccgccaga aactgatcta gagtcggggc ggccggccgc ttcgagcaga catgataaga 3061 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 3121 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 3181 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 3241 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt 3301 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 3361 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg 3421 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 3481 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4321 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag 4381 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc 4441 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg 4501 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat 4561 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat 4621 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg 4681 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc 4741 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa 4801 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc 4861 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt 4921 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag 4981 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt 5041 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag 5101 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac 5161 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc 5221 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca 5281 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga 5341 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat 5401 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat 5461 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct //