LOCUS       V029777                 4716 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029777
VERSION     V029777
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..2227
                     /label="DCAF13(NM_015420)"
                     /note="DCAF13(NM_015420)"
                     /gene="DCAF13"
     polyA_signal    2270..2391
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2810..3398)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3598..4458)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    4563..4611
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    4625..4716
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgaaggt
      901 gaagatgctg agccggaatc cggacaatta tgtccgcgaa accaagttgg acttacagag
      961 agttccaaga aactatgatc ctgctttaca tccttttgag gtcccacgag aatatataag
     1021 agctttaaat gctaccaaac tggaacgagt atttgcaaaa ccattccttg cttcgctgga
     1081 tggtcaccgt gatggagtca attgcttggc aaagcatcca gagaagctgg ctactgtcct
     1141 ttctggggcg tgtgatggag aggttagaat ttggaatcta actcagcgga attgtatccg
     1201 tacaatacaa gcacatgaag gctttgtacg aggaatatgt actcgctttt gtgggacttc
     1261 ttttttcact gttggtgatg acaaaactgt gaagcagtgg aaaatggatg ggccaggcta
     1321 tggagacgag gaagagccat tacatacaat attaggaaag acagtgtata ctgggattga
     1381 tcatcactgg aaagaagctg tttttgccac atgtggacag caagtagaca tttgggatga
     1441 acaaagaact aatcctatat gttcaatgac ctggggattt gacagtataa gtagtgttaa
     1501 atttaaccca attgagacat ttctcttggg aagttgtgca tctgacagga atatagtact
     1561 gtacgatatg aggcaagcta ctcctttgaa aaaggttatc ttagatatga gaacaaatac
     1621 aatctgttgg aaccctatgg aagctttcat ttttacagca gcaaatgaag attataactt
     1681 atatactttt gatatgcgtg cactggacac tcctgtaatg gtccatatgg atcatgtatc
     1741 tgcagtgctt gatgtggatt actctcccac tgggaaggag tttgtgtctg ctagtttcga
     1801 taaatctatt cgaatctttc ctgtagacaa aagtcgaagc agggaggtat atcatacaaa
     1861 gagaatgcaa catgttatct gtgtaaaatg gacttctgac agcaagtata ttatgtgtgg
     1921 atctgatgaa atgaacattc gcctgtggaa agctaatgct tctgaaaaat tgggtgtgct
     1981 tacatcacga gaaaaagcag ccaaggatta taaccagaaa ttgaaggaga aatttcagca
     2041 ttatcctcat ataaaacgta tagctcgtca tcgacatcta ccaaaatcta tctatagcca
     2101 gattcaggaa cagcgcatca tgaaagaagc tcgtcgacga aaggaagtga atcgtattaa
     2161 acacagcaag cctggatctg tgccacttgt gtcagagaag aagaaacacg tagtggcagt
     2221 tgtaaaataa tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     2281 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2341 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2401 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2461 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc
     2521 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     2581 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     2641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3541 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     3601 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     3661 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     3721 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     3781 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     3841 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     3901 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     3961 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4021 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     4081 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     4141 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     4201 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     4261 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     4321 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     4381 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     4441 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     4501 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     4561 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     4621 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     4681 tccccagtgc aagtgcaggt gccagaacat ttctct
//
