LOCUS       V030007                 5730 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030007
VERSION     V030007
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..3241
                     /label="CDK11A(NM_001313896)"
                     /note="CDK11A(NM_001313896)"
                     /gene="CDK11A"
     polyA_signal    3284..3405
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3824..4412)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4612..5472)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    5577..5625
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    5639..5730
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgggtga
      901 tgaaaaggac tcttggaaag tgaaaacttt agatgaaatt cttcaggaaa agaaacgaag
      961 gaaggaacaa gaggagaaag cagagataaa acgcttaaaa aattctgatg accgggattc
     1021 caagcgggat tcccttgagg agggggagct gagagatcac tgcatggaga tcacaataag
     1081 gaactccccg tatagaagag aagactcaat ggaagacaga ggagaagaag atgattcttt
     1141 ggccatcaaa ccaccccagc aaatgtctcg gaaagaaaaa gttcatcaca gaaaagatga
     1201 aaagagaaaa gaaaaatgga agcatgctag agtgaaagaa agagagcacg aacgtcggaa
     1261 acgacatcga gaagaacagg ataaagctcg ccgggaatgg gaaagacaga agagaaggga
     1321 aatggcaagg gagcattcca ggagagaaag ggaccgcttg gagcagttag aaaggaagcg
     1381 ggagcgggag cgcaagatgc gggagcagca gaaggagcag cgggagcaga aggagcgcga
     1441 gcggcgggcg gaggagcggc gcaaggagcg ggaggcccgc agggaagtgt ctgcacatca
     1501 ccgaacgatg agagaggact acagcgacaa agtgaaagcc agccactgga gtcgcagccc
     1561 gcctcggccg ccgcgggagc ggttcgagtt gggagacggc cggaagccag gtgaggccag
     1621 gccggcgcct gcgcagaagc cagcacagtt aaaagaagag aaaatggaag aaagggacct
     1681 gctgtccgac ttacaggaca tcagcgacag cgagaggaag accagctcgg ccgagtcctc
     1741 gtcagcggaa tcaggctcag gttctgagga agaagaggag gaggaggaag aggaggagga
     1801 ggaagggagc accagtgaag aatcagagga ggaggaggag gaagaggaag aggaggagga
     1861 ggagaccggc agcaactctg aggaggcatc agagcagtct gccgaagaag taagtgagga
     1921 agaaatgagt gaagatgaag aacgagaaaa tgaaaaccac ctcttggttg ttccagagtc
     1981 acggttcgac cgagattccg gggagagtga agaagcagag gaagaagtgg gtgagggaac
     2041 gccgcagagc agcgccctga cagagggcga ctatgtgccc gactcccctg ccctgttgcc
     2101 catcgagctc aagcaggagc tgcccaagta cctgccggcc ctgcagggct gccggagcgt
     2161 cgaggagttc cagtgcctga acaggatcga ggagggcacc tatggagtgg tctacagagc
     2221 aaaagacaag aaaacagatg aaattgtggc tctaaagcgg ctgaagatgg agaaggagaa
     2281 ggagggcttc ccgatcacgt ccctgaggga gatcaacacc atcctcaagg cccagcatcc
     2341 caacattgtc accgttagag agattgtggt gggcagcaac atggacaaga tctacatcgt
     2401 gatgaactac gtggagcacg acctcaagag cctgatggag accatgaaac agcccttcct
     2461 gccaggggag gtgaagaccc tgatgatcca gctgctgcgg ggggtgaaac acctgcacga
     2521 caactggatc ctgcaccgtg acctcaagac gtccaacctg ctgctgagcc acgccggcat
     2581 cctcaaggtg ggtgattttg ggctggcgcg ggagtacgga tcccctctga aggcctacac
     2641 cccggtcgtg gtgacccagt ggtaccgcgc cccagagctg ctgcttggtg ccaaggaata
     2701 ctccacggcc gtggacatgt ggtcagtggg ctgcatcttc ggggagctgc tgactcagaa
     2761 gcctctgttc cccgggaatt cggaaatcga tcagatcaac aaagtgttca aggagctggg
     2821 gacccccagt gagaaaatct ggcccggcta cagtgagctc ccagtagtca aaaagatgac
     2881 cttcagcgag cacccctaca acaacctccg caagcgcttc ggggctctgc tctcagacca
     2941 gggcttcgac ctcatgaaca agttcctgac ctacttcccc gggaggagga tcagcgctga
     3001 ggacggcctc aagcatgagt atttccgcga gacccccctc cccatcgacc cctccatgtt
     3061 ccccacgtgg cccgccaaga gcgagcagca gcgtgtgaag cggggcacca gcccgaggcc
     3121 ccctgaggga ggcctgggct acagccagct gggtgacgac gacctgaagg agacgggctt
     3181 ccaccttacc accacgaacc agggggcctc tgccgcgggc cccggcttca gcctcaagtt
     3241 ctgatctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag
     3301 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3361 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3421 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     3481 ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa
     3541 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     3601 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
     3661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3961 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4021 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4081 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4141 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4201 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4261 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4321 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4381 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4441 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4501 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4561 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
     4621 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
     4681 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
     4741 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
     4801 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
     4861 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
     4921 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
     4981 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
     5041 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
     5101 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
     5161 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
     5221 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
     5281 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
     5341 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
     5401 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
     5461 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
     5521 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
     5581 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
     5641 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
     5701 gtgcaagtgc aggtgccaga acatttctct
//
