LOCUS       V029722                 5145 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029722
VERSION     V029722
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..2656
                     /label="CAMK2G(NM_001367534)"
                     /note="CAMK2G(NM_001367534)"
                     /gene="CAMK2G"
     polyA_signal    2699..2820
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3239..3827)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4027..4887)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    4992..5040
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    5054..5145
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatggccac
      901 caccgccacc tgcacccgtt tcaccgacga ctaccagctc ttcgaggagc ttggcaaggg
      961 tgctttctct gtggtccgca ggtgtgtgaa gaaaacctcc acgcaggagt acgcagcaaa
     1021 aatcatcaat accaagaagt tgtctgcccg ggatcaccag aaactagaac gtgaggctcg
     1081 gatatgtcga cttctgaaac atccaaacat cgtgcgcctc catgacagta tttctgaaga
     1141 agggtttcac tacctcgtgt ttgaccttgt taccggcggg gagctgtttg aagacattgt
     1201 ggccagagag tactacagtg aagcagatgc cagccactgt atacatcaga ttctggagag
     1261 tgttaaccac atccaccagc atgacatcgt ccacagggac ctgaagcctg agaacctgct
     1321 gctggcgagt aaatgcaagg gtgccgccgt caagctggct gattttggcc tagccatcga
     1381 agtacaggga gagcagcagg cttggtttgg ttttgctggc accccaggtt acttgtcccc
     1441 tgaggtcttg aggaaagatc cctatggaaa acctgtggat atctgggcct gcggggtcat
     1501 cctgtatatc ctcctggtgg gctatcctcc cttctgggat gaggatcagc acaagctgta
     1561 tcagcagatc aaggctggag cctatgattt cccatcacca gaatgggaca cggtaactcc
     1621 tgaagccaag aacttgatca accagatgct gaccataaac ccagcaaagc gcatcacggc
     1681 tgaccaggct ctcaagcacc cgtgggtctg tcaacgatcc acggtggcat ccatgatgca
     1741 tcgtcaggag actgtggagt gtttgcgcaa gttcaatgcc cggagaaaac tgaagggtgc
     1801 catcctcacg accatgcttg tctccaggaa cttctcagtt ggcaggcaga gctccgcccc
     1861 cgcctcgcct gccgcgagcg ccgccggcct ggccgggcaa gctgccaaaa gcctattgaa
     1921 caagaagtcg gatggcggtg tcaagaaaag gaagtcgagt tccagcgtgc acctaatgcc
     1981 acagagcaac aacaaaaaca gtctcgtaag cccagcccaa gagcccgcgc ccttgcagac
     2041 ggccatggag ccacaaacca ctgtggtaca caacgctaca gatgggatca agggctccac
     2101 agagagctgc aacaccacca cagaagatga ggacctcaaa gctgccccgc tccgcactgg
     2161 gaatggcagc tcggtgcctg aaggacggag ctcccgggac agaacagccc cctctgcagg
     2221 catgcagccc cagccttctc tctgctcctc agccatgcga aaacaggaga tcattaagat
     2281 tacagaacag ctgattgaag ccatcaacaa tggggacttt gaggcctaca cgaagatttg
     2341 tgatccaggc ctcacttcct ttgagcctga ggcccttggt aacctcgtgg aggggatgga
     2401 tttccataag ttttactttg agaatctcct gtccaagaac agcaagccta tccataccac
     2461 catcctaaac ccacacgtcc acgtgattgg ggaggacgca gcgtgcatcg cctacatccg
     2521 cctcacccag tacatcgacg ggcagggtcg gcctcgcacc agccagtcag aagagacccg
     2581 ggtctggcac cgtcgggatg gcaagtggct caatgtccac tatcactgct caggggcccc
     2641 tgccgcaccg ctgcagtgat ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     2701 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2761 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2821 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2881 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     2941 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     3001 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     3061 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3121 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3181 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3241 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3301 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3361 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3421 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3481 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3541 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3601 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3661 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3721 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3781 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3841 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3901 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3961 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     4021 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     4081 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     4141 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     4201 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     4261 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     4321 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     4381 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     4441 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     4501 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     4561 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     4621 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     4681 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     4741 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     4801 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     4861 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     4921 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     4981 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     5041 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     5101 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
