LOCUS       pBiT2.1-AHCY(human)     4677 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029735
VERSION     V029735
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..2188
                     /label="AHCY(NM_000687)"
                     /note="AHCY(NM_000687)"
                     /gene="AHCY"
     polyA_signal    2231..2352
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2771..3359)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3559..4419)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    4524..4572
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    4586..4677
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgtctga
      901 caaactgccc tacaaagtcg ccgacatcgg cctggctgcc tggggacgca aggccctgga
      961 cattgctgag aacgagatgc cgggcctgat gcgtatgcgg gagcggtact cggcctccaa
     1021 gccactgaag ggcgcccgca tcgctggctg cctgcacatg accgtggaga cggccgtcct
     1081 cattgagacc ctcgtcaccc tgggtgctga ggtgcagtgg tccagctgca acatcttctc
     1141 cacccaggac catgcggcgg ctgccattgc caaggctggc attccggtgt atgcctggaa
     1201 gggcgaaacg gacgaggagt acctgtggtg cattgagcag accctgtact tcaaggacgg
     1261 gcccctcaac atgattctgg acgacggggg cgacctcacc aacctcatcc acaccaagta
     1321 cccgcagctt ctgccaggca tccgaggcat ctctgaggag accacgactg gggtccacaa
     1381 cctctacaag atgatggcca atgggatcct caaggtgcct gccatcaatg tcaatgactc
     1441 cgtcaccaag agcaagtttg acaacctcta tggctgccgg gagtccctca tagatggcat
     1501 caagcgggcc acagatgtga tgattgccgg caaggtagcg gtggtagcag gctatggtga
     1561 tgtgggcaag ggctgtgccc aggccctgcg gggtttcgga gcccgcgtca tcatcaccga
     1621 gattgacccc atcaacgcac tgcaggctgc catggagggc tatgaggtga ccaccatgga
     1681 tgaggcctgt caggagggca acatctttgt caccaccaca ggctgtattg acatcatcct
     1741 tggccggcac tttgagcaga tgaaggatga tgccattgtg tgtaacattg gacactttga
     1801 cgtggagatc gatgtcaagt ggctcaacga gaacgccgtg gagaaggtga acatcaagcc
     1861 gcaggtggac cggtatcggt tgaagaatgg gcgccgcatc atcctgctgg ccgagggtcg
     1921 gctggtcaac ctgggttgtg ccatgggcca ccccagcttc gtgatgagta actccttcac
     1981 caaccaggtg atggcgcaga tcgagctgtg gacccatcca gacaagtacc ccgttggggt
     2041 tcatttcctg cccaagaagc tggatgaggc agtggctgaa gcccacctgg gcaagctgaa
     2101 tgtgaagttg accaagctaa ctgagaagca agcccagtac ctgggcatgt cctgtgatgg
     2161 ccccttcaag ccggatcact accgctactg atctagagtc ggggcggccg gccgcttcga
     2221 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2281 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2341 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     2401 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
     2461 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     2521 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     2581 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2641 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2701 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2761 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2821 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2881 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2941 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3001 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3061 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3121 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3181 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3241 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3301 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3361 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3421 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3481 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat
     3541 gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg
     3601 cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga
     3661 gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc
     3721 cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac
     3781 tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc
     3841 agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc
     3901 gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc
     3961 catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt
     4021 ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc
     4081 atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg
     4141 tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag
     4201 cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat
     4261 cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc
     4321 atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa
     4381 gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta
     4441 ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt
     4501 acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     4561 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
     4621 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct
//
