LOCUS       pBiT2.1-AFAP1(human)    5823 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029779
VERSION     V029779
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..3334
                     /label="AFAP1(NM_001134647)"
                     /note="AFAP1(NM_001134647)"
                     /gene="AFAP1"
     polyA_signal    3377..3498
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3917..4505)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4705..5565)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    5670..5718
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    5732..5823
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatggaaga
      901 gttaatagtt gaacttcgtc tctttcttga actcctggac catgaatatc taacctcaac
      961 tgtcagggag aaaaaggcag tgataaccaa cattctgcta agaatacagt catccaaagg
     1021 ttttgatgtg aaggaccatg ctcagaagca ggagaccgct aacagcctgc cagcccctcc
     1081 tcagatgccc ctgccggaga tccctcagcc ctggctgcct cctgacagtg ggcctccacc
     1141 attgccaaca tcctccctcc cagaaggtta ttatgaggaa gctgtgccgc tgagccccgg
     1201 aaaagctccg gaatacatca catcaaatta tgattccgat gcgatgagca gctcttatga
     1261 gtcgtatgat gaagaggagg aggatgggaa ggggaagaaa acccggcacc agtggccctc
     1321 cgaggaggcc tccatggacc tggtcaagga cgccaaaatc tgcgccttcc tgctgcggaa
     1381 gaagcggttc ggccagtgga ccaagttgct ctgcgtcatc aaagacacca aactgctgtg
     1441 ctataaaagt tccaaggacc agcagcctca gatggaactg ccactccaag gctgtaacat
     1501 tacgtacatc ccgaaagaca gcaaaaagaa gaagcacgag ctgaagatta ctcagcaggg
     1561 cacggacccg cttgttctcg ccgtccagag caaggaacag gccgagcagt ggctgaaggt
     1621 gatcaaagaa gcctacagtg gttgtagtgg ccccgtggat tcagagtgtc ctcctccacc
     1681 aagctccccg gtgcacaagg cagaactgga gaagaaactg tcttcagaga gacccagctc
     1741 agatggggag ggtgttgtgg aaaatggaat taccacatgt aatggaaagg agcaagtgaa
     1801 gaggaagaaa agttccaaat cagaggccaa gggcactgtg tcgaaagtca ctgggaaaaa
     1861 aatcaccaag atcatcagtc tgggaaagaa aaagccgtcc acagacgagc agacctcctc
     1921 agctgaggaa gatgttccca cctgcggcta tctgaacgtg ctctccaaca gccgctggcg
     1981 agagcgctgg tgccgagtga aagataacaa gctcattttc cacaaggaca ggaccgacct
     2041 gaagacccat attgtgtcta ttccgctccg tggctgcgag gtgatcccgg gtttggattc
     2101 taaacatcct ctgacgttcc ggctgctgcg caacggccag gaggttgcag tattggaggc
     2161 atcttcttct gaagacatgg gcaggtggat tgggatttta ctcgcagaga cgggatcgtc
     2221 cacagacccg gaggctctgc actatgacta cattgatgtg gagatgtctg caagtgtcat
     2281 tcagacagcc aaacagacct tctgtttcat gaacaggcgt gttatatctg ctaacccata
     2341 tctagggggc acctccaacg gctatgccca ccccagcggg acggcacttc attatgacga
     2401 tgtcccgtgc atcaacggct cgtgggaacc ggaagacggc tttcctgctt cctgcagcag
     2461 aggcttggga gaagaggtgc tttatgataa cgcaggcctg tacgataact tgccgcctcc
     2521 gcacatcttt gcccgctact ctcctgctga cagaaaggcc tctaggctgt ctgctgacaa
     2581 gctgtcctct aaccattaca aataccctgc ctccgctcag tctgtcacta atacctcttc
     2641 tgtggggagg gcgtctctcg ggctcaactc gcagctcaag ggtaaaaagc cccccgtggc
     2701 gtctaatggg gtcacaggaa aagggaagac tctgagcagt cagccaaaga aagcggatcc
     2761 cgcggctgtt gtgaaaagga cgggttcgaa tgctgcccag tacaagtatg gcaagaaccg
     2821 ggtagaagca gatgccaagc ggctacagac caaagaggag gagctgctga agaggaaaga
     2881 ggccctgcgg aataggctgg cccagctccg caaggaaaga aaagaccttc gagcggctat
     2941 tgaagtgaac gccggcagga agccgcaggc gatcctggag gagaagctga agcagctgga
     3001 ggaggagtgc cggcagaagg aggcggagcg tgtcagcctg gagctggagc tgacggaggt
     3061 caaggagagc ctgaagaaag cgctggcggg cggagtcacc ctggggctgg ccatcgagcc
     3121 caagtcaggg acatcgagtc cacagtctcc agtgttccgg caccggaccc tggaaaactc
     3181 gcccatctcc agctgtgaca ccagtgacac cgagggcccc gtgccggtga acagcgcggc
     3241 cgtcttgaag aagagccagg ctgccccggg cagctccccc tgccgagggc atgtgctgcg
     3301 gaaggccaag gaatgggaat tgaagaacgg gacctagtct agagtcgggg cggccggccg
     3361 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3421 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3481 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3541 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     3601 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     3661 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     3721 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3781 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3841 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4381 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4501 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4561 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4621 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc
     4681 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc
     4741 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat
     4801 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc
     4861 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc
     4921 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag
     4981 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg
     5041 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg
     5101 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag
     5161 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt
     5221 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga
     5281 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc
     5341 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc
     5401 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc
     5461 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc
     5521 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca
     5581 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat
     5641 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt
     5701 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
     5761 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     5821 tct
//
