LOCUS       40924_6492       12925 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBiFCt-2in1-NN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Grefen C, Blatt MR.
  TITLE     A 2in1 cloning system enables ratiometric bimolecular fluorescence 
            complementation (rBiFC)
  JOURNAL   BioTechniques 53 (5), 311-314 (2012)
  PUBMED    23066669
REFERENCE   2  (bases 1 to 12925)
  AUTHORS   Grefen C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2015) ZMBP Developmental Genetics, University of 
            Tuebingen, Auf der Morgenstelle 32, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 12925)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2012"; volume: "53"; issue: "5"; pages: 
            "311-314"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2015) ZMBP Developmental Genetics, University of Tuebingen, 
            Auf der Morgenstelle 32, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        93..437
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             531..995
                     /label=VN155(I152L)
                     /note="improved N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
     CDS             1029..1055
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    1066..1189
                     /label=attR3
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1224..1254
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1262..1278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1286..1302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1298..1516
                     /codon_start=1
                     /product="lacZ"
                     /label=lacZ
                     /protein_id="AMD02807.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRS"
     protein_bind    complement(1563..1687)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1719..1924
                     /label=T35S terminator
                     /note="T35S terminator"
                     /regulatory_class="terminator"
     regulatory      1937..2439
                     /label=CaMV35S plus Omega enhancer
                     /note="CaMV35S plus Omega enhancer"
                     /regulatory_class="promoter"
     promoter        2014..2358
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2446..3120
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     regulatory      3177..3385
                     /label=T35S terminator
                     /note="T35S terminator"
                     /regulatory_class="terminator"
     promoter        3475..3819
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3910..4161
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     CDS             4195..4227
                     /codon_start=1
                     /product="MYC-tag"
                     /label=MYC-tag
                     /protein_id="AMD02810.1"
                     /translation="MEQKLISEEDL"
     CDS             4198..4227
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    4246..4370
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4395..4425
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4479..5135
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             5480..5782
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5826..5950)
                     /label=attR4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      5982..6187
                     /label=T35S terminator
                     /note="T35S terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(6275..6299)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6820..7608
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      7857..8445
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8631..8771)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9115..9309)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9378..10448)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10880..11506)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(12837..12861)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 actagccctc tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc
       61 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
      121 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
      181 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
      241 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
      301 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      361 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      421 tttcatttgg agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca
      481 aacaacaaca acattacatt ttacattcta caactacatc tagtcaattg atggtgagca
      541 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      601 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      661 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      721 ccttcggcta cggcctgcag tgcttcgcca ggtaccccga ccacatgaag cagcacgact
      781 tcttcaagtc cgccatgccc gaaggctacg tccaggagag aaccatcttc ttcaaggacg
      841 acggcaacta caagaccagg gccgaggtga agttcgaggg cgacaccctg gtgaacagaa
      901 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      961 acaactacaa cagccacaac gtctatatca tggccggagg tggatctgga ggtggaagtc
     1021 agaacatgta tccttatgat gttcctgatt atgctagtca attggcaact ttgtataata
     1081 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     1141 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcggg
     1201 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1261 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta ccccgtccct
     1321 gcacgcctgt cgttcaaccc tggaagatcc gcgtgtcccg agttctaaca gtctggccgt
     1381 cgttctgcag cgtcgcgatt gggaaaaccc gggcgtgacc caactgaatc gtctggcggc
     1441 ccatccgccg tttgcgtcgt ggcgtaactc agaagaagcc cgtaccgacc gtccgtctca
     1501 acaactgcgc tcctaacact ataaaaagct gatgcagacg gggctttttt ctgcaggtcg
     1561 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     1621 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     1681 aagtggtgtc taggttataa gcggccatgc tagagtccgc aaaaatcacc agtctctctc
     1741 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     1801 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     1861 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     1921 gtgacctagg agggctgtca acatggtgga gcacgacact ctcgtctact ccaagaatat
     1981 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     2041 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2101 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     2161 tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa
     2221 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     2281 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2341 atttcatttg gagaggacct cgagcgcgcg tatttttaaa caattaccaa caacaacaac
     2401 aaacaacaac aacattacat tttacattct acaactacat ctagtatggc ctcctccgag
     2461 gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     2521 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2581 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2641 cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2701 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2761 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2821 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2881 tccaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gatgaggctg
     2941 aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag
     3001 cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac
     3061 gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc
     3121 caattgttaa tcacaagttt gtacaaagtg gtgattaagc ggccatgcta gagtccgcaa
     3181 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     3241 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     3301 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     3361 tcctaaaacc aaaatccagt gaccttaccc tctgcaggtc aacatggtgg agcacgacac
     3421 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac
     3481 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     3541 cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa
     3601 aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc
     3661 cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     3721 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     3781 ttcctctata taaggaagtt catttcattt ggagaggacc tcgagcgcgc gtatttttaa
     3841 acaattacca acaacaacaa caaacaacaa caacattaca ttttacattc tacaactaca
     3901 tctaggatgg acaagcagaa gaacggcatc aaggtgaact tcaagatcag gcacaacatc
     3961 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4021 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     4081 aacgagaaga gagatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4141 ggcatggacg agctgtacaa gtctggaggt ggatctggag gtggaagtag gccaatggaa
     4201 cagaagctta tctcagagga ggacctggcc ggcactagtc ctagaacaag tttgtacaaa
     4261 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     4321 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     4381 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     4441 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     4501 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     4561 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     4621 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     4681 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4741 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4801 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4861 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4921 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     4981 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     5041 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     5101 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     5161 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     5221 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     5281 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     5341 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     5401 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     5461 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     5521 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     5581 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
     5641 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     5701 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     5761 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     5821 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     5881 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca acttttctat
     5941 acaaagttgg tcaattgtta taagcggcca tgctagagtc cgcaaaaatc accagtctct
     6001 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     6061 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     6121 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     6181 ccagtgacct aggagggtaa gtcctcaatt cggcgttaat tcagtacatt aaaaacgtcc
     6241 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     6301 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     6361 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     6421 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     6481 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     6541 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     6601 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     6661 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     6721 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     6781 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     6841 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     6901 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     6961 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     7021 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     7081 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     7141 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     7201 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     7261 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     7321 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     7381 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     7441 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     7501 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     7561 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     7621 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     7681 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     7741 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     7801 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7981 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8221 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8521 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8581 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     8641 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8701 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8761 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8821 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8881 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     8941 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     9001 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     9061 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     9121 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     9181 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     9241 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     9301 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     9361 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     9421 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     9481 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     9541 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     9601 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     9661 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     9721 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     9781 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     9841 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     9901 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     9961 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
    10021 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
    10081 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    10141 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    10201 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    10261 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    10321 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    10381 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    10441 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10501 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    10561 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    10621 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    10681 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10741 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10801 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    10861 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    10921 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    10981 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    11041 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    11101 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    11161 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    11221 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    11281 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    11341 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    11401 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    11461 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    11521 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    11581 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    11641 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    11701 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11761 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    11821 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    11881 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    11941 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    12001 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    12061 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    12121 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    12181 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    12241 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    12301 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    12361 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    12421 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12481 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    12541 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    12601 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    12661 ctatagcagc ggaggggttg gatcaaagta ctttaaagta ctttaaagta ctttaaagta
    12721 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    12781 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt
    12841 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
    12901 tgatccaagc tcaagctaag ctagg
//
