LOCUS       62056_4025        4460 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..30
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             505..756
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     polyA_signal    complement(771..905)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1016..1032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1040..1056)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1064..1094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1109..1130)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1418..2006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2180..3037)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3038..3142)
                     /label=AmpR promoter
     primer_bind     3616..3632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        3659..3962
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3963..4166
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          4304..4400
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattcagatg
       61 cagacagccc ccgtgcccat gccagacctg aagaatgtca agtccaagat cggctccact
      121 gagaacctga agcaccagcc gggaggcggg aaggtgcaga taattaataa gaagctggat
      181 cttagcaacg tccagtccaa gtgtggctca aaggataata tcaaacacgt cccgggaggc
      241 ggcagtgtgc aaatagtcta caaaccagtt gacctgagca aggtgacctc caagtgtggc
      301 tcattaggca acatccatca taaaccagga ggtggccagg tggaagtaaa atctgagaag
      361 cttgacttca aggacagagt ccagtcgaag attgggtccc tggacaatat cacccacgtc
      421 cctggcggag gaaataaaaa gattgaaggt acccgtccgg cgtgcaaaat cccgaacgac
      481 ctgaaacaga aagtcatgaa ccacgacaag cagaagaacg gcatcaaggc caacttcaag
      541 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
      601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaaa
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      721 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcggg gatccagaca
      781 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      841 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      901 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      961 ttttttcgga tcctctagag tcgatctgca ggcatgctag cttggcgtaa tcatggtcat
     1021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1081 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1141 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1201 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3181 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     3241 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3301 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3361 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     3421 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     3481 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     3541 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     3601 cccagtcacg acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg
     3661 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3721 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3781 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3841 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3901 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3961 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     4021 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     4081 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     4141 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     4201 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cggactctag
     4261 aggatccggt actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
     4321 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
     4381 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
     4441 attgtacccg cgggcccacc
//
