LOCUS       .                       8582 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     141..157
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     enhancer        318..697
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             935..958
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4394..4912
                     /label="VN173"
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     polyA_signal    4923..5545
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     promoter        5574..5903
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(5942..5960)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5974..5990)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5998..6014)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6022..6052)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6067..6088)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6376..6964)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7138..7995)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7996..8100)
                     /label="AmpR promoter"
     rep_origin      8127..8582
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tttgcggccg caagccaaaa tggaggtgaa gacgtcactt ctggacaata tgattggagt
     1021 tggagatatg gttcttttag aacctctcaa tgaggaaact gtcatcaaca acctcaagaa
     1081 gcgctttgac catagcgaga tatatacata cattggaagt gtggttatat ctgttaaccc
     1141 ataccggtcc ttgcccattt attcaccaga gaaagtggaa gattacagga acagaaattt
     1201 ttatgaactg agccctcaca tctttgccct gtcagatgaa gcatacagat ccctaagaga
     1261 tcaagataaa gaccagtgta ttctgattac tggggaaagt ggagcaggaa aaacagaggc
     1321 cagcaagctt gttatgtcct acgtagcggc tgtttgcgga aagggagcag aagtgaatca
     1381 agtcaaagaa cagcttttac agtccaaccc tgtcctggaa gcttttggaa acgccaagac
     1441 tgtaaggaat gacaactctt ctagatttgg caaatatatg gatattgaat tcgattttaa
     1501 aggcgatccg ctgggaggag tgataagtaa ctatctttta gagaaatctc gagtcgttaa
     1561 gcagccaaga ggtgaaagaa acttccacgt gttctatcag ctgctttctg gtggctctga
     1621 agagctcctc aataagctta aacttgagcg agatttcagc agatacaact acctgagtct
     1681 ggactcagcc aaagtgaatg gagtggatga tgcagcaaat ttcagaaccg ttaggaatgc
     1741 catgcagatt gtgggtttca tggatcatga agctgagtct gtcttggagg tggtggcggc
     1801 agtgttgaag ctggggaaca ttgagttcaa gcctgaatct cgagtgaatg gtttagatga
     1861 aagcaagatc aaagataaaa atgagttaaa agaaatttgc gagctgactg gcattgatca
     1921 gtcagttcta gaacgagcct tcagcttccg aacagttgag gccaagcaag agaaagtttc
     1981 gactacactg aacgtggctc aggcttatta tgcccgtgat gctctggcca aaaacctcta
     2041 cagcaggttg ttttcatggt tggtaaatcg aatcaatgaa agcattaagg cacaaacaaa
     2101 agtaagaaag aaggtcatgg gtgttttgga catttatggc ttcgaaattt ttgaggacaa
     2161 cagctttgag cagttcatta ttaattattg taatgaaaag ctgcaacaaa tcttcattga
     2221 actgactctt aaagaagagc aggaggagta tatacgggag ggcatagaat ggactcacat
     2281 tgaatacttc aataatgcta tcatatgtga cctgatagaa aataacacaa acggaatcct
     2341 ggccatgctg gatgaggagt gcctgaggcc cggcaccgtc accgacgaga ccttcttgga
     2401 aaagctgaac caagtgtgcg ccacccacca gcactttgag agcaggatga gcaagtgctc
     2461 ccggttcctc aatgacacgt ccctgcctca cagctgcttc agaatccagc attatgctgg
     2521 caaggtactg taccaggtgg aaggatttgt ggataagaac aatgaccttc tctaccgaga
     2581 cctgtcccaa gccatgtgga aggccagcca cgccctcatc aagtctttgt tccctgaggg
     2641 gaaccctgcc aagatcaacc tgaaaaggcc tcctacagct ggctcccagt tcaaggcatc
     2701 tgtggccact ctgatgaaaa acctccagac caagaatcca aactacatta ggtgtatcaa
     2761 accgaatgat aaaaaagccg cacacatctt caacgagggt ctggtgtgtc atcagatcag
     2821 gtacctgggt cttttggaga atgtccgcgt gaggagggcg ggctatgcct tcaggcaggc
     2881 ctacgaacct tgcctggaaa gatacaaaat gctgtgtaaa caaacatggc ctcattggaa
     2941 aggaccagca agagctggtg tggaggtttt gtttaatgaa ttggagattc ctgtggaaga
     3001 atactccttt ggtagatcaa agatattcat tcgaaaccca agaacattat tcaagttgga
     3061 agacctgagg aagcagcgtc ttgaggacct ggccaccctc attcagaaga tttatcgggg
     3121 gtggaaatgt cgcacacact tcctcctgat gagaaaaagc caaattgtga tcgccgcctg
     3181 gtacaggaga tacgcgcaac aaaagaggta tcaacagata aagagctcgg ccttggtaat
     3241 tcagtcttat atccggggtt ggaaggcccg aaaaatcctg cgggaactga agcatcaaaa
     3301 gcgctgtcag gaagcagcca ccaccattgc agcttattgg catggtaccc aggcgcgaag
     3361 ggaactgagc cggctgaagg cggaggctag gaataaacat gctattgcag ttatttgggc
     3421 ttactggctt ggatctaagg ctcgaaggga gttgaaacgc ttgaaggaag aggctaggcg
     3481 taagcatgca gttgctgtca tttgggctta ctggcttgga ctgaaggtac gcagggagta
     3541 taggaaattc ttcagagcca atgctggaaa gaaaatttat gaatttacgc ttcagagaat
     3601 tatgcaaaaa tacttcttgg aaatgaaaaa taaggtgcct tccctatctc cgatagacaa
     3661 gaattggcca tcaagacctt acctattctt ggattctact cacaaggagc taaaaaggat
     3721 tttccacttg tggaggtgta aaaaatacag ggaccagttc acagaccaac agaaacttat
     3781 ttacgaagag aaactagaag ccagtgaact cttcaaagac aagaaggctc tatacccatc
     3841 tagtgttggg caaccattcc aagggcctta cctggaaatc aacaagaatc ccaagtataa
     3901 gaaactcaag gatgccattg aagaaaagat tatcattgct gaagttgtga acaaaattaa
     3961 ccgtgctaat gggaagagta caaaccggat tttcctcttg acaaataata atcttcttct
     4021 tgctgatcaa aagtctgggc agatcaagtc agaggttccc ctggtggatg taaccaaagt
     4081 atcaatgagc tcgcaaaatg atggcttctt tgccatccac ctcaaagagg gctctgaagc
     4141 agcaagtaaa ggagactttc tcttcagcag tgatcacctg attgaaatgg ccaccaagct
     4201 ctaccggacg actctcagcc aaaccaaaca gaagctcaat attgaaattt ccgatgagtt
     4261 cctggtgcag ttcagacagg acaaagtatg tgtgaagttt attcagggca accagaaaaa
     4321 tgggagtgtc ccaacgtgca agcgaaaaaa caaccgtctc cttgaagttg ctgttcctag
     4381 atccatcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4441 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4501 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
     4561 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
     4621 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4681 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4741 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4801 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
     4861 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg agtagggatc
     4921 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     4981 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     5041 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     5101 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     5161 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     5221 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     5281 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     5341 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     5401 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc
     5461 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
     5521 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc
     5581 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5641 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5701 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5761 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5821 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5881 ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt
     5941 ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     6001 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6061 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6121 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8101 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     8161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8521 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8581 tt
//
