LOCUS       40924_6357        5038 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBhSV-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 5038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky Mountain 
            Laboratories, National Institute of Allergy and Infectious Diseases,
            National Institutes of Health, 903 S. 4th Street, Hamilton, MT 
            59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(790..1161)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     CDS             complement(1421..2227)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(2228..2348)
                     /label=flgB promoter derived from Borrelia hermsii
                     /note="flgB promoter derived from Borrelia hermsii"
                     /regulatory_class="promoter"
     gene            complement(2605..3114)
                     /pseudo
                     /gene="vmp"
                     /label=vmp
                     /note="orfC'"
     RBS             2684..2692
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3281..4375
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="similar to gene family 57; orfA"
                     /protein_id="ABS71230.1"
                     /translation="MGNTKKFTNKYQHKLIVLISTLNYMNLKLKKYTQNDILYYFNNNM
                     KKNDQNPIKLKTLQSYLYKLKKEFQVTINYHRHLGVNMGTEIHYELKYSKKECYCIINK
                     QFREKKEERHKKRVNVYLEKTCIKNSSVEKWECSYNIYNNKEEKKDIKEIEKLQVKKYI
                     RKCNFKSDILYSILDLELEKNATIKVCKIIKRTENFIENSIYKRINGIKSNRSKQRELS
                     KILNETRIRLENEGHNGKQLETQIQEVYEQYKNKPHFIIENNKYNDLKKIIGKLKKTVE
                     YTNRNAKENERDVRNNVFSILLEQLRHKVDKSILVSILKGYLNKQDKLTYSKALNNYYY
                     HELLELINNNLDYLKPEKLEIITS"
     CDS             4385..4945
                     /codon_start=1
                     /gene="orfB"
                     /product="hypothetical protein"
                     /label=orfB
                     /note="simlar to gene family 50; orfB"
                     /protein_id="ABS71231.1"
                     /translation="MESILERLKKKESEIKKKNNRNLFVKVEKINNRTIYHTKIMKDLF
                     SFGINKNQRGKFFVSFRELFNQEKIAVFNLFSLRDDDKFLGISYWYRKPIQNVVTRYEE
                     NGIMKASTFSKVYYVEFRFKKGSVCCYIGEITYLLRKEKANKKYYESLVERMINLEKQV
                     YEFYGKKLPNGGIINKWIEKNQK"
     gene            4385..4945
                     /gene="orfB"
                     /label=orfB
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttag
      781 cacgtgtcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag
      841 ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc cggaggcgtc
      901 ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca ggccgcgcac
      961 ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc tgatgaacag
     1021 ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc
     1081 gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac
     1141 ggcactggtc aacttggcca tggtggccct cctcacgtgc tattattgaa gcatttatca
     1201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     1261 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat
     1321 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgcc gtcccgtcaa gtcagcgtaa
     1381 tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
     1441 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     1501 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     1561 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     1621 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     1681 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     1741 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     1801 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     1861 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     1921 tttttccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     1981 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     2041 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     2101 catacaagcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     2161 catataaatc agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat
     2221 ggctcatatg aacaccctct atatcacaaa ttgcttttta ctatattata taataattta
     2281 aatctttttg ttcgttaatt tttacttttt cattaacata gtccaagttt atttcaattt
     2341 tctttaacgc cggcaagggc gaattctgca gatatccatc acactggcgg ccgctaagtc
     2401 caaaagcgtc cccaatagca ttaccaaaaa tgccaaaaat ctcttgaaac ccatgtccta
     2461 tcttaactaa tgaatctaag aaagtattct tactttcagc ggccaatttc tcagattgaa
     2521 aataaaaaga aaacaattct catgaagaaa aaagttttcc tagaatgact ttatttggtt
     2581 atgtatattg ttattaaatt ctaactaatt cttcttagct tcagaggttg tattatcaat
     2641 aattaaagga gtatcattag gattaatttt tattgcttct ttaacctcct taagtcctac
     2701 gtcaattgtt tttcttattg ctatggtgag agtatccaat gctttagtta ctgcacttat
     2761 tgctgctccc ttaactgctt tctccacatc aacatcacca ttattaacct tagcaaattt
     2821 accatccttt gccatagctc ttaacactat acctcctgct acttctgcat ctttagaagc
     2881 attgacacca gcaacttgag cggcattatt ctcagctaat ttaacagcac ttccattatc
     2941 tttaatcata gcttgcaaaa tatcagcacc agttacagca ccaactgctt ttgctgcatc
     3001 agctgctgat ttctttgcat cagaagcagc tccagtacta ctattaaaca atttacctgc
     3061 gccgtcatta ttctctgcgc tattaccatc ttcagctttt ttattatccc cacctcagga
     3121 tttcctttgt ctttaagtaa gaaatattac caactagatc accaacagta ccctataatg
     3181 tatttactaa aaatattttt attataaatg ggtttacttt ttaacgggta ttgcactatg
     3241 ataaaagata ataattcaat aaccaacaca ggagatttaa atggggaaca caaaaaaatt
     3301 tacaaacaaa taccaacaca aattaatagt tttaatatca acactaaatt acatgaactt
     3361 aaagttaaaa aaatacactc aaaatgacat actttattat ttcaataata atatgaaaaa
     3421 aaatgaccaa aaccctatca aacttaaaac actacaaagt tatctttata aattaaaaaa
     3481 agaatttcaa gtaacaatta attatcacag acatttgggt gttaacatgg gaactgaaat
     3541 tcactacgaa cttaaatact ctaaaaaaga atgttactgc ataatcaata aacaatttag
     3601 agaaaaaaaa gaggaaagac ataaaaaacg tgttaatgta tatcttgaaa agacttgtat
     3661 taaaaatagc agtgtagaaa aatgggagtg ttcttataat atatataata ataaagaaga
     3721 gaagaaagac ataaaagaaa tagaaaaact acaagtaaaa aaatacatca gaaaatgcaa
     3781 ctttaaatca gacatactct attctattct agatttagaa ttagaaaaaa atgctacgat
     3841 taaagtatgt aaaattatta aaagaaccga aaatttcatt gaaaatagta tttacaagag
     3901 aattaatggt attaaatcga acagaagtaa acagcgagaa ttaagcaaga tcttgaatga
     3961 aacaaggatt agattagaaa atgaaggaca caacggtaaa cagttagaaa cacaaataca
     4021 agaagtatat gaacaatata aaaacaaacc ccacttcatt atagagaata acaagtataa
     4081 tgacttaaag aaaataatag gaaagcttaa aaaaacagtt gaatatacta atagaaacgc
     4141 aaaagaaaat gagagagacg ttagaaataa cgtatttagt atacttcttg aacaattaag
     4201 acataaagta gacaaatcga ttttagtatc aatattgaag ggttatttaa ataaacagga
     4261 taagttaaca tacagcaagg cacttaataa ttattactac catgaacttt tagagttgat
     4321 aaataataat ctagattatt taaaaccaga aaaacttgag ataatcacca gttaaggatt
     4381 agttatggag agcatattag aacgcttaaa aaaaaaggaa tcagaaatta agaaaaaaaa
     4441 caacaggaat cttttcgtga aagtagaaaa aattaataac agaacaatat accatacaaa
     4501 aataatgaaa gatttgttct cttttgggat taataaaaac caaagaggta aatttttcgt
     4561 ttcctttagg gaacttttta atcaagaaaa aatagcggtg tttaatctgt tttctttaag
     4621 agatgatgat aaatttttgg gcatatctta ttggtataga aaaccaatac aaaatgttgt
     4681 aacaagatat gaagagaatg gtatcatgaa agcgtccaca ttttcaaaag tttattacgt
     4741 agaatttaga tttaagaaag gtagtgtttg ttgttatatt ggagaaatta cttatttact
     4801 tagaaaggaa aaagctaata agaaatatta tgaatcttta gttgaaagaa tgatcaattt
     4861 agaaaaacaa gtatatgaat tttatggcaa aaagctacca aatggaggaa ttataaataa
     4921 atggatagaa aaaaaccaga aataatcaca attgctaata tcaagggtgg tgttggaaaa
     4981 agcattttga ctattatttt tagttatatt ttaaaagatg cgggcaagaa gattttag
//
